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Merge pull request #16 from NIGMS/fix_broken_links
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Fix broken links
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kyleoconnell-NIH authored Oct 7, 2024
2 parents 567bc32 + ba05629 commit 83835d7
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2 changes: 1 addition & 1 deletion reusable-workflow-repo
Submodule reusable-workflow-repo updated from e24998 to c4c1c8
10 changes: 7 additions & 3 deletions tutorial_1-bismark.ipynb
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"! ~/miniconda3/bin/conda init bash\n",
"! ~/miniconda3/bin/conda init zsh\n",
"``` \n",
"It would take less time to install some of the tools in this tutorial if you create a new conda environment. But it is a little bit tricky to use a different conda environment within Jupyter in GCP. You can find instructions of how to create a new conda environment [here](https://github.com/STRIDES/NIHCloudLabGCP/docs/How_to_use_conda_envs_as_kernels.ipynb).\n",
"It would take less time to install some of the tools in this tutorial if you create a new conda environment. But it is a little bit tricky to use a different conda environment within Jupyter in GCP. You can find instructions of how to create a new conda environment [here](https://github.com/STRIDES/NIHCloudLabGCP/blob/main/docs/create_conda_env.md).\n",
"</div>"
]
},
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"\n",
"All four DNA strands that arise through bisulfite treatment and subsequent PCR amplification can be sequenced with the same frequency in non-directional libraries, while for directional libraries, adapters are attached to the DNA fragments such that only the original top or bottom strands will be sequenced. In the Bismark alignment step, `--directional` is set to default, so only report OT and OB strands.\n",
"\n",
"**Output alignment**. The output .bam files are the binary version of the SAM (Sequence Alignment/Map) format. Please see [here](https://github.com/FelixKrueger/Bismark/tree/master/Docs#bismark-bamsam-output-default) for more detailed explanation of the output .bam/.sam format, where the `XM-tag` is the methylation call string to indicate the methylated status of each C(tyosine) in different contexts: CG (or CpG), CHG or CHH (where H correspond to A, T or C). The methylation call string contains a dot ‘.’ for every position in the BS-read not involving a cytosine,\n",
"**Output alignment**. The output .bam files are the binary version of the SAM (Sequence Alignment/Map) format. Please see [here](https://github.com/FelixKrueger/Bismark/blob/master/docs/bismark/alignment.md#bismark-bamsam-output-default) for more detailed explanation of the output .bam/.sam format, where the `XM-tag` is the methylation call string to indicate the methylated status of each C(tyosine) in different contexts: CG (or CpG), CHG or CHH (where H correspond to A, T or C). The methylation call string contains a dot ‘.’ for every position in the BS-read not involving a cytosine,\n",
"or contains one of the following letters (z,Z,x,X,h,H) for the three different cytosine methylation contexts (UPPER CASE = METHYLATED, lower case = unmethylated).\n",
"> <img src=\"images/1_bismark_bam.png\" width=\"600\" />\n",
"\n",
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]
}
],
"metadata": {},
"metadata": {
"language_info": {
"name": "python"
}
},
"nbformat": 4,
"nbformat_minor": 5
}

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