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Getting started

FLIMinator edited this page Feb 13, 2023 · 4 revisions

dOPM concept

dOPM paper

Software requirements

Need to install:

  • Nikon NIS-Elements
  • Other software

Setup

  • Setup NIS-Elements configuration

Running

  • Snap an image with wide-field camera

dOPM acquisition

  • concept of view 1 & 2 offsets
  • how NIS-elements handles the linear actuator that does dOPM remote refocus scanning
  • how to combine wide-field right-hand-port (RHP) XYZ information with dOPM left-hand-port (LHP) acquisition