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Update README.md
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willem-stock authored Jul 1, 2024
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Expand Up @@ -78,5 +78,15 @@ A result directory will be created in which there will be the most relevant file
The reads that failed to map to the consensus sequences are in the unmapped folder


* the output files can be further processed with phyloseq in R (an r script is available in this repository to get you started: CONCOMPRA_local_postprocessing.R)

The output files can be further processed with phyloseq in R (an r script is available in this repository to get you started: CONCOMPRA_local_postprocessing.R)
Alternatively, you can use vsearch (which is present in the CONCOMPRA environment) to assign a taxonomy to your consensus sequences.
For this, you'll have to:
* download one of the [available reference databases](https://www.drive5.com/usearch/manual/sintax_downloads.html)
* create aa UDB database file from the database file
```
vsearch -makeudb_usearch $DATABASE.gz -output $DATABASE.udb
```
* generate taxonomic annotations
```
vsearch --sintax $FASTA_FILE --db $DATABASE.udb --tabbedout reads.sintax
```

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