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# Open Soure Hardware Optics and Mechanics Design for Fluorescence Activated (Microfluidic) Droplet Sorting (FADS) [![Open Source Love](https://badges.frapsoft.com/os/v1/open-source.svg?v=103)](https://github.com/ellerbrock/open-source-badges/)

It in cludes the design of positioning, lasers, filters, detectors, fixtures for open droplet sorting. Note that the design in this repository is quite different to literatures designs. The set-up has been re-designed in order to make it more low-cost (estimated parts cost 20.000 USD, mainly for optical filters, detectors and laser) and open source, including hardware, software and accessible fabrication of parts where possible. Also, a number of updates and improvements have been made over the current state-of the art of droplet sorters, inspired by technical advances in the related field of flow-cytometry.
# EMTY REPOSITORY - NOW EMBEDDED IN THE [MAIN DROPLET SORTER REPOSITORY](https://github.com/wenzel-lab/droplet-sorter-master)

Follow us! [#twitter](https://twitter.com/WenzelLab), [#YouTube](https://www.youtube.com/@librehub), [#LinkedIn](https://www.linkedin.com/company/92802424), [#instagram](https://www.instagram.com/wenzellab/), [#Printables](https://www.printables.com/@WenzelLab), [#LIBREhub website](https://librehub.github.io), [#IIBM website](https://ingenieriabiologicaymedica.uc.cl/en/people/faculty/821-tobias-wenzel)


## Related documents:

* The microfluidic chip is positioned with an [open source SQUID microscopy stage](https://github.com/wenzel-lab/squid-motorized-stage).
* And the otptical detection arm assembly is based on the [Open-UC2 3d printed optics cubes](https://github.com/openUC2/UC2-GIT).
* The modified and new Open-UC2 compatible cubes can be found in the STL folder of this repository, and all modifyable design files are openly availble in the cloud [here on OnShape](https://tinyurl.com/WenzelLabUC2), where you can view, export and copy (to modify) the project designs.

A few examples of modified or new designs for this project:

<p align="center">
<img src="./images/filter_cube_finished_(green_blue).jpg" height="360">
<img src="./images/objective_insert_as_filter_in_cube.jpg" height="360">
</p>
<p align="center">
<img src="./images/detector_SiPM_holder_assembled.jpg" height="310">
<img src="./images/mirror_mount_with_heat_inserts_assembled.jpg" height="310">
</p>
<p align="center">
<img src="./images/detection_arm_assembly.jpg" height="400">
</p>

## Contribute

This is an open project in the Wenzel Lab in Santiago, Chile. If you have any suggestions to improve it or add any additional functions make a pull-request or [open an issue](https://github.com/wenzel-lab/droplet-cytometry-optomechanics/issues/new).
For interactions in our team and with the community applies the [GOSH Code of Conduct](https://openhardware.science/gosh-2017/gosh-code-of-conduct/).

## License

[CERN OHL 2W](LICENSE) © Tobias Wenzel. This project is Open Source Hardware - please acknowledge us when using the hardware or sharing modifications.

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