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Code for designing CRISPEY-BAR library and data processing of sequencing reads for yeast epistasis project

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crispey3-epistasis

Code for designing CRISPEY-BAR library and data processing of sequencing reads for yeast epistasis project.

The required packages for running most scripts are listed in the crispey-bar environment file.

To run DESeq2 in R, use crispey-R environment.

To run Azimuth step in oligo design pipeline, run crispey3_add_guide_features.py in crispey-py27 environment.

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Code for designing CRISPEY-BAR library and data processing of sequencing reads for yeast epistasis project

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