Nextflow stdout:

WARN: The following invalid input values have been detected:

* --baseDir: /home/runner/work/ampliseq/ampliseq
* --base-dir: /home/runner/work/ampliseq/ampliseq
* --outputDir: /home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/output
* --output-dir: /home/runner/work/ampliseq/ampliseq/.nf-test/tests/e65899427d65947658e67cf1532e2d6e/output


WARN: No DADA2 cutoffs were specified (`--trunclenf` & `--trunclenr`), therefore reads will be truncated where median quality drops below 25 (defined by `--trunc_qmin`) but at least a fraction of 0.75 (defined by `--trunc_rmin`) of the reads will be retained.
The chosen cutoffs do not account for required overlap for merging, therefore DADA2 might have poor merging efficiency or even fail.

WARN: The operator `first` is useless when applied to a value channel which returns a single value by definition
WARN: Probably everything is fine, but this is a reminder that `--trunclenf` was set automatically to 230 and `--trunclenr` to 229. If this doesnt seem reasonable, then please change `--trunc_qmin` (and `--trunc_rmin`), or set `--trunclenf` and `--trunclenr` directly.
WARN: QIIME2_DIVERSITY_CORE: The sampling depth of 500 seems too small for rarefaction
Nextflow stderr: