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Co-authored-by: Till E. <[email protected]>
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d4straub and tillenglert authored Oct 19, 2023
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2 changes: 1 addition & 1 deletion README.md
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## Introduction

**nfcore/ampliseq** is a bioinformatics analysis pipeline used for amplicon sequencing, supporting denoising of any amplicon and taxonomic assignment. Phylogenetic placement is also possible. Supported is paired-end Illumina or single-end Illumina, PacBio and IonTorrent data. Default is the analysis of 16S rRNA gene amplicons sequenced paired-end with Illumina.
**nfcore/ampliseq** is a bioinformatics analysis pipeline used for amplicon sequencing, supporting denoising of any amplicon and supports a variety of taxonomic databases for taxonomic assignment including 16S, ITS, CO1 and 18S. Phylogenetic placement is also possible. Supported is paired-end Illumina or single-end Illumina, PacBio and IonTorrent data. Default is the analysis of 16S rRNA gene amplicons sequenced paired-end with Illumina.

A video about relevance, usage and output of the pipeline (version 2.1.0; 26th Oct. 2021) can also be found in [YouTube](https://youtu.be/a0VOEeAvETs) and [billibilli](https://www.bilibili.com/video/BV1B44y1e7MM), the slides are deposited at [figshare](https://doi.org/10.6084/m9.figshare.16871008.v1).

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4 changes: 2 additions & 2 deletions conf/modules.config
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]
}

withName: 'PHYLOSEQ' {
withName: PHYLOSEQ {
publishDir = [
path: { "${params.outdir}/phyloseq" },
mode: params.publish_dir_mode,
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]
}

withName: 'MULTIQC' {
withName: MULTIQC {
ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : ''
publishDir = [
path: { "${params.outdir}/multiqc" },
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4 changes: 2 additions & 2 deletions docs/usage.md
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```bash
nextflow run nf-core/ampliseq \
-r 2.6.1 \
-r 2.7.0 \
-profile singularity \
--input "samplesheet.tsv" \
--FW_primer GTGYCAGCMGCCGCGGTAA \
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--outdir "./results"
```

In this example, `--input` is the [Samplesheet input](#samplesheet-input), other options are [Direct FASTQ input](#direct-fastq-input) and [ASV/OTU fasta input](#asvotu-fasta-input). For more details on metadata, see [Metadata](#metadata). For [Reproducibility](#reproducibility), specify the version to run using `-r` (= release, e.g. 2.6.1, please use the most recent release). See the [nf-core/ampliseq website documentation](https://nf-co.re/ampliseq/parameters) for more information about pipeline specific parameters.
In this example, `--input` is the [Samplesheet input](#samplesheet-input), other options are [Direct FASTQ input](#direct-fastq-input) and [ASV/OTU fasta input](#asvotu-fasta-input). For more details on metadata, see [Metadata](#metadata). For [Reproducibility](#reproducibility), specify the version to run using `-r` (= release, e.g. 2.7.0, please use the most recent release). See the [nf-core/ampliseq website documentation](https://nf-co.re/ampliseq/parameters) for more information about pipeline specific parameters.

It is possible to not provide primer sequences (`--FW_primer` & `--RV_primer`) and skip primer trimming using `--skip_cutadapt`, but this is only for data that indeed does not contain any PCR primers in their sequences. Also, metadata (`--metadata`) isnt required, but aids downstream analysis.

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