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TACAlgorithm

Computer Vision ImageJ Macro to label gold particles in immunogold labeled EM images

  1. Applies a fixed parameter gray-level threshold to the image.
  2. Remaining binary components are filtered between a predetermined minimum and maximum area parameter.
  • These parameters are chosen based on the known sizes of gold particle contained in the image (e.g., 6 and 12 nm).
  1. A circularity threshold is applied to each component to validate the common roundness of colloidal gold particles.

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Computer Vision ImageJ Macro to label gold particles in immunogold labeled EM images

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