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Using custom SAM tags with TALON

Dana Wyman edited this page Mar 20, 2020 · 2 revisions

TALON recognizes and stores the following custom SAM tags in the observed table of the database on a per-read basis. The values of these tags are also reported in the read_annot output file.

Tag Type Description
fA:f float Fraction As in post-alignment window
lC:Z string Custom label
lA:Z string Allelic label
tS:Z string Transcript start support
tE:Z string Transcript end support

Currently, only one of these tags is in active use by the TALON pipeline, namely the fA tag for internal priming detection. We added the lA, tS, and tE tags to help support features in the future such as allele-based quantification or more sophisticated transcript filtering. The custom lC tag is intended to allow users to record SAM information of their choice to pass into TALON.