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Adding confidence level as a parameter #121

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May 30, 2024
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2 changes: 1 addition & 1 deletion DESCRIPTION
Original file line number Diff line number Diff line change
@@ -1,6 +1,6 @@
Package: miaViz
Title: Microbiome Analysis Plotting and Visualization
Version: 1.13.1
Version: 1.13.2
Authors@R:
c(person(given = "Tuomas", family = "Borman", role = c("aut", "cre"),
email = "[email protected]",
Expand Down
1 change: 1 addition & 0 deletions NEWS
Original file line number Diff line number Diff line change
Expand Up @@ -27,3 +27,4 @@ Changes in version 1.11.x

Changes in version 1.13.x
+ plot*Tree: bugfix, ununique nodes
+ Added confidence.interval parameter to plotCCA
36 changes: 21 additions & 15 deletions R/plotCCA.R
Original file line number Diff line number Diff line change
Expand Up @@ -24,6 +24,9 @@
#'
#' @param ellipse.linetype Discrete number specifying the style of ellipses.
#' (default: \code{ellipse.linetype = 1})
#'
#' @param confidence.level Number between 0 and 1 to adjust confidence level.
#' (default: \code{confidence.level = 0.95})
#'
#' @param add.vectors TRUE or FALSE, should vectors appear in the plot.
#' (default: \code{add.vectors = TRUE})
Expand Down Expand Up @@ -179,8 +182,8 @@ setGeneric("plotRDA", signature = c("object"),
#' @export
setMethod("plotRDA", signature = c(object = "SingleCellExperiment"),
function(object, dimred,
add.ellipse = TRUE, ellipse.alpha = 0.2, ellipse.linewidth = 0.1, ellipse.linetype = 1,
vec.size = 0.5, vec.color = vec.colour, vec.colour = "black", vec.linetype = 1,
add.ellipse = TRUE, ellipse.alpha = 0.2, ellipse.linewidth = 0.1, ellipse.linetype = 1,
confidence.level = 0.95, vec.size = 0.5, vec.color = vec.colour, vec.colour = "black", vec.linetype = 1,
arrow.size = 0.25, label.color = label.colour, label.colour = "black", label.size = 4,
vec.text = TRUE, repel.labels = TRUE, sep.group = "\U2014", repl.underscore = " ",
add.significance = TRUE, add.expl.var = TRUE, add.vectors = TRUE, parse.labels = TRUE, ...){
Expand Down Expand Up @@ -219,19 +222,22 @@ setMethod("plotRDA", signature = c(object = "SingleCellExperiment"),
if( !.are_whole_numbers(ellipse.linetype) ){
stop("'vec.linetype' must be a whole number.", call. = FALSE)
}
if( ellipse.alpha < 0 || ellipse.alpha > 1 ){
if ( !(is.numeric(ellipse.alpha) && ellipse.alpha > 0 && ellipse.alpha < 1 ) ) {
stop("'ellipse.alpha' must be a number between 0 and 1.", call. = FALSE)
}
if ( !is.numeric(ellipse.linewidth) && ellipse.linewidth > 0 ) {
if ( !(is.numeric(ellipse.linewidth) && ellipse.linewidth > 0) ) {
stop("'ellipse.linewidth' must be a positive number.", call. = FALSE)
}
if ( !is.numeric(vec.size) && vec.size > 0 ) {
if( !(is.numeric(confidence.level) && confidence.level > 0 && confidence.level < 1) ) {
stop("'confidence.level' must be a number between 0 and 1.", call. = FALSE)
}
if ( !(is.numeric(vec.size) && vec.size > 0) ) {
stop("'vec.size' must be a positive number.", call. = FALSE)
}
if ( !is.numeric(arrow.size) && arrow.size > 0 ) {
if ( !(is.numeric(arrow.size) && arrow.size > 0) ) {
stop("'arrow.size' must be a positive number.", call. = FALSE)
}
if ( !is.numeric(label.size) && label.size > 0 ) {
if ( !(is.numeric(label.size) && label.size > 0) ) {
stop("'label.size' must be a positive number.", call. = FALSE)
}
if ( !.is_non_empty_string(vec.color) ) {
Expand All @@ -256,11 +262,11 @@ setMethod("plotRDA", signature = c(object = "SingleCellExperiment"),
# Create a plot
plot <- .rda_plotter(
plot_data, ellipse.alpha = ellipse.alpha, ellipse.linewidth = ellipse.linewidth,
ellipse.linetype = ellipse.linetype, vec.size = vec.size, vec.color = vec.color,
vec.colour = vec.colour, vec.linetype = vec.linetype, arrow.size = arrow.size,
label.color = label.color, label.colour = label.colour, label.size = label.size,
vec.text = vec.text, add.ellipse = add.ellipse, repel.labels = repel.labels,
parse.labels = parse.labels, ...
ellipse.linetype = ellipse.linetype, confidence.level = confidence.level, vec.size = vec.size,
vec.color = vec.color, vec.colour = vec.colour, vec.linetype = vec.linetype,
arrow.size = arrow.size, label.color = label.color, label.colour = label.colour,
label.size = label.size, vec.text = vec.text, add.ellipse = add.ellipse,
repel.labels = repel.labels, parse.labels = parse.labels, ...
)
return(plot)
}
Expand Down Expand Up @@ -493,7 +499,7 @@ setMethod("plotRDA", signature = c(object = "matrix"),
# Plot based on the data
#' @importFrom ggrepel geom_text_repel geom_label_repel
.rda_plotter <- function(
plot_data, ellipse.alpha = 0.2, ellipse.linewidth = 0.1, ellipse.linetype = 1,
plot_data, ellipse.alpha = 0.2, ellipse.linewidth = 0.1, ellipse.linetype = 1, confidence.level = 0.95,
vec.size = 0.5, vec.color = vec.colour, vec.colour = "black",
vec.linetype = 1, arrow.size = 0.25, min.segment.length = 5,
label.color = label.colour, label.colour = "black", label.size = 4,
Expand All @@ -516,12 +522,12 @@ setMethod("plotRDA", signature = c(object = "matrix"),
aes(x = .data[[xvar]], y = .data[[yvar]],
color = .data[[colour_var]], fill = after_scale(color)),
geom = "polygon", alpha = ellipse.alpha,
linewidth = ellipse.linewidth, linetype = ellipse.linetype)
linewidth = ellipse.linewidth, linetype = ellipse.linetype, level = confidence.level)
} else if ( add.ellipse %in% c("color", "colour") ){
plot <- plot +
stat_ellipse(data = data,
aes(x = .data[[xvar]], y = .data[[yvar]], color = .data[[colour_var]]),
geom = "polygon", alpha = 0, linetype = ellipse.linetype)
geom = "polygon", alpha = 0, linetype = ellipse.linetype, level = confidence.level)
}

}
Expand Down
4 changes: 4 additions & 0 deletions man/plotCCA.Rd

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