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Update readme
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mourisl committed Jul 20, 2023
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6 changes: 6 additions & 0 deletions README.md
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Expand Up @@ -166,6 +166,12 @@ The last step of generating simple report can be done with the command:
perl trust-simplerep.pl trust_cdr3.out > trust_report.out

If you are interested in a subset of chains, you can "grep" those from trust_cdr3.out and run trust-simplerep.pl on the subset.

* #### Annotation only

You can use the "annotator" from TRUST4 to annotate the V,D,J,C genes and CDRs for any given sequences, just like using IgBLAST or IMGT/VQuest. To obtain the annotation in AIRR format for human sequences with eight threads, you can use the command

./annotator -f human_IMGT+C.fa -a input.fa --fasta -t 8 --needReveserComplement --noImpute --outputFormat 1 > annotation.tsv

### Example

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2 changes: 1 addition & 1 deletion run-trust4
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Expand Up @@ -7,7 +7,7 @@ use Cwd 'cwd' ;
use Cwd 'abs_path' ;
use File::Basename ;

die "TRUST4 v1.0.10.1-r454 usage: ./run-trust4 [OPTIONS]:\n".
die "TRUST4 v1.0.11-r458 usage: ./run-trust4 [OPTIONS]:\n".
"Required:\n".
#"\t[Input]:\n".
"\t-b STRING: path to bam file\n".
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