polyester_gen

This is a script to generate reads with package polyester which simulates DEG reads

Requires an annotation file and a fasta file with the transcript sequences.

Usage

0. Clean annotation with the python script.
1. Source the R file in the terminal after choosing between interactive and default. Interactive will allow more customization of parameters while default will generate reads with the following parameters: read length: 35 pair ended: false error model: uniform, 0% stranded: true
2. Test favorite tools.

Features

Customizable config with the main parameters from the package.

Choose where you want your reads to be put

Cohort Reads 1

Generation features

• No merged features
• No duplicated features (i.e. large repeats)