Processing Dcytb data (5ZLE & 5ZLG)

The following describes how duodenal cytochrome b (Dcytb) datasets can be processed using KAMO (documentation in Japanese / English).

References

• Original paper
  • Ganasen et al. (2018) "Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate." Communications Biology doi: 10.1038/s42003-018-0121-8
• Structures described here
  • substrate-free form at 2.6 Å PDB: 5ZLE
  • complex with Zn and ascorbate at 2.8 Å PDB: 5ZLG

Raw data

• Available in SBGrid Data Bank under CC0
  • ID 573 (substrate-free form, 5ZLE) doi: 10.15785/SBGRID/573
  • ID 574 (complex with Zn and ascorbate, 5ZLG) doi: 10.15785/SBGRID/574
• Collected on BL32XU, SPring-8 using 1 Å wavelength and 5×5 μm² beam
• C2, a~65, b~116, c~48 Å, β~118°
• substrate-free form (5ZLE)
  • 1548 small-wedge (5°/dataset) datasets with EIGER X 9M detector
• complex with Zn and ascorbate (5ZLG)
  • 1370 small-wedge (5°/dataset) datasets with EIGER X 9M detector

How data were processed in the original paper

Processing of individual datasets

Merging