Processing Chrimson data (5ZIH)

The following describes how Chrimson datasets can be processed using KAMO (documentation in Japanese / English).

References

• Original paper
  • Oda et al. (2018) "Crystal structure of the red light-activated channelrhodopsin Chrimson." Nature Communications doi: 10.1038/s41467-018-06421-9 PDB: 5ZIH

Raw data

• Available in Zenodo.
• Collected on BL32XU, SPring-8
• EIGER X 9M detector, ??? μm² beam, 1 Å wavelength, ??? mm camera length
• 45 small-wedge (10° or 20° each) datasets collected manually from ?? cryoloops
• 8 helical (50-100° each) datasets collected manually from ?? cryoloops
• P2₁2₁2₁; a= 61.0, b= 81.4, c= 170.1 Å

How data were processed in the original paper