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NTLEN definition in pindel2vcf4tcga output #114

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abolia opened this issue Nov 13, 2019 · 0 comments
Open

NTLEN definition in pindel2vcf4tcga output #114

abolia opened this issue Nov 13, 2019 · 0 comments

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@abolia
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abolia commented Nov 13, 2019

Hi,

I am trying to understand the definition of NTLEN in the pindel vcf output. The header says that it is: "Number of bases inserted in place of deleted code". It makes sense for RPL variants where a chunk of bases are deleted and a sequence is inserted.
However, in duplications based on the figure 2e: http://gmt.genome.wustl.edu/packages/pindel/new-development.png in Pindel's user manual, it looks like its just an inserted sequence between reference and duplicated sequence. There isnt anything deleted here. So, this definition seems applicable only to RPL variants and not when we observe non template sequence for duplicated events. I just wanted to clarify so that I am not missing anything.

Thank you.
Ashini

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