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wolski authored May 31, 2024
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# How to get started

[![SIB in-silico talk](https://img.youtube.com/vi/acDiXq2xbOw/1.jpg)](https://www.youtube.com/watch?v=acDiXq2xbOw)

- Watch the [silico talks](https://www.sib.swiss/in-silico-talks/prolfqua-a-comprehensive-r-package-for-protein-differential-expression-analysis)
- See our article at the [Journal of Proteome Research](https://pubmed.ncbi.nlm.nih.gov/36939687/)
- See [Bioconductor 2021 Conference poster](https://fgcz-proteomics.uzh.ch/~wolski/PosterBioconductor.html).
- Watch the lightning (8 min) talk at [EuroBioc2020](https://www.youtube.com/watch?v=jOXU4X7nV9I&t) on YouTube or [slides](https://f1000research.com/slides/9-1476).
- Read the pkgdown generate website https://fgcz.github.io/prolfqua/index.html


How to build a LFQData object for a table with protein or peptide quantification results and an annotation file with the explanatory variables
is described in the: [CreatingConfigurations vignette](https://fgcz.github.io/prolfqua/articles/CreatingConfigurations.html)


How to build a `LFQData` object from a table with protein or peptide quantification results, and an table with sample annotation
is described in more detail here the: [CreatingConfigurations vignette](https://fgcz.github.io/prolfqua/articles/CreatingConfigurations.html)


Or run the following example code:
Once you have created an `LFQData` you can use prolfqua like this.

```{r}
R.version.string; packageVersion("prolfqua")
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![image](https://github.com/fgcz/prolfqua/assets/1926513/4ae8634b-ce6c-4fa2-8e42-c8bc64a12821)


Detailed documentation with R code:
[![SIB in-silico talk](https://img.youtube.com/vi/acDiXq2xbOw/1.jpg)](https://www.youtube.com/watch?v=acDiXq2xbOw)

- Watch the [silico talks](https://www.sib.swiss/in-silico-talks/prolfqua-a-comprehensive-r-package-for-protein-differential-expression-analysis)
- See our article at the [Journal of Proteome Research](https://pubmed.ncbi.nlm.nih.gov/36939687/)
- See [Bioconductor 2021 Conference poster](https://fgcz-proteomics.uzh.ch/~wolski/PosterBioconductor.html).
- Watch the lightning (8 min) talk at [EuroBioc2020](https://www.youtube.com/watch?v=jOXU4X7nV9I&t) on YouTube or [slides](https://f1000research.com/slides/9-1476).
- Read the pkgdown generate website https://fgcz.github.io/prolfqua/index.html


# Detailed documentation with R code:

- [Comparing two Conditions](https://fgcz.github.io/prolfqua/articles/Comparing2Groups.html)
- [QC and protein wise sample size estimation](https://fgcz.github.io/prolfqua/articles/QualityControlAndSampleSizeEstimation.html)
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