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(1) Basecalling

High accurarcy basecalling with Guppy. Execute ClusterBasecalling.sh on the Cluster. You need to modify the parameters in the first rows of the script

(2) NanoPlot

Run NanoPlot analysis of the BaseCalled reads, or subsets of it. The information to be passed to the script Nanoplot.sh needs to be provided in a sequencing_summary.txt as generated by the Guppy pipeline. A typical commandline looks like this:

sh shell/Nanoplot.sh \
path/to/outputfolder \
path/to/sequencing_summary.txt \
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  • Shell 80.7%
  • Python 19.3%