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Merge with offset fix
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andy-sweet committed May 15, 2024
1 parent 7f24349 commit 753a82e
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Showing 4 changed files with 25 additions and 25 deletions.
39 changes: 19 additions & 20 deletions src/napari_cryoet_data_portal/_open_widget.py
Original file line number Diff line number Diff line change
Expand Up @@ -34,16 +34,6 @@ class Resolution:
indices: Tuple[int, ...]
scale: float

@property
def offset(self) -> float:
"""The offset due to a larger first pixel for lower resolutions.
When visualized in napari, this ensures that the different multi-scale
layers opened separately share the same visual extent in the canvas that
starts at (-0.5, -0.5, -0.5).
"""
return (self.scale - 1) / 2


MULTI_RESOLUTION = Resolution(name="Multi", indices=(0, 1, 2), scale=1)
HIGH_RESOLUTION = Resolution(name="High", indices=(0,), scale=1)
Expand Down Expand Up @@ -139,7 +129,8 @@ def _loadTomogram(
) -> Generator[FullLayerData, None, None]:
logger.debug("OpenWidget._loadTomogram: %s", tomogram.name)
image_layer = read_tomogram(tomogram)
# Extract image_scale before the resolution is taken into account.
# Extract image_scale before the resolution is taken into account,
# so we can use it to align other annotations later.
image_scale = image_layer[1]["scale"]
yield _handle_image_at_resolution(image_layer, resolution)

Expand All @@ -155,9 +146,9 @@ def _loadTomogram(
for annotation in annotations:
for layer in read_annotation_files(annotation, tomogram=tomogram):
if layer[2] == "labels":
layer = _handle_image_at_resolution(layer, resolution=resolution)
layer = _handle_image_at_resolution(layer, resolution)
elif layer[2] == "points":
layer = _handle_points_annotation(layer, image_scale)
layer = _handle_points_at_scale(layer, image_scale)
yield layer

def _onLayerLoaded(self, layer_data: FullLayerData) -> None:
Expand Down Expand Up @@ -186,17 +177,25 @@ def _handle_image_at_resolution(layer_data: FullLayerData, resolution: Resolutio
data = np.asarray(data)

# Adjust the scale and and translation based on the resolution.
attrs["scale"] = tuple(resolution.scale * s for s in attrs["scale"])
image_translate = attrs.get("translate", (0,) * len(attrs["scale"]))
attrs["translate"] = tuple(resolution.offset + t for t in image_translate)
image_scale = attrs["scale"]
attrs["scale"] = tuple(resolution.scale * s for s in image_scale)
# Offset the translation due to a larger first pixel for lower resolutions.
# When visualized in napari, this ensures that the different multi-scale
# layers opened separately share the same visual extent in the canvas that
# starts at some scaled version of (-0.5, -0.5, -0.5).
image_translate = attrs.get("translate", (0,) * len(image_scale))
attrs["translate"] = tuple(
(s * (resolution.scale - 1) / 2) + t
for s, t in zip(image_scale, image_translate)
)
return data, attrs, layer_type


def _handle_points_annotation(layer_data: FullLayerData, image_scale: Tuple[float, float, float]) -> FullLayerData:
def _handle_points_at_scale(layer_data: FullLayerData, image_scale: Tuple[float, float, float]) -> FullLayerData:
data, attrs, layer_type = layer_data
# Inherit scale from full resolution image so that we can pick up
# that scale when it changes.
# Inherit scale from full resolution image, so that points are visually
# aligned with the image.
attrs["scale"] = image_scale
# Scaling points also changes the size, so adjust accordingly.
attrs["size"] /= np.mean(image_scale)
return data, attrs, layer_type
return data, attrs, layer_type
4 changes: 2 additions & 2 deletions src/napari_cryoet_data_portal/_sample_data.py
Original file line number Diff line number Diff line change
Expand Up @@ -36,12 +36,12 @@ def _read_tomogram_from_10000(name: str) -> List[FullLayerData]:
ribosome_annotations = [
item
for item in annotations
if item.object_name.lower() == "cytosolic ribosome"
if "cytosolic ribosome" in item.object_name.lower()
].pop()
fas_annotations = [
item
for item in annotations
if item.object_name.lower() == "fatty acid synthase"
if "fatty acid synthase" in item.object_name.lower()
].pop()
ribosome_points = read_annotation(ribosome_annotations, tomogram=tomogram)
fatty_acid_points = read_annotation(fas_annotations, tomogram=tomogram)
Expand Down
4 changes: 2 additions & 2 deletions src/napari_cryoet_data_portal/_tests/test_open_widget.py
Original file line number Diff line number Diff line change
Expand Up @@ -36,7 +36,7 @@ def test_set_tomogram_adds_layers_to_viewer(widget: OpenWidget, tomogram: Tomogr
with qtbot.waitSignal(widget._progress.finished, timeout=30000):
widget.setTomogram(tomogram)

assert len(widget._viewer.layers) == 9
assert len(widget._viewer.layers) > 1


def test_set_tomogram_adds_layers_to_viewer_without_clearing_existing(widget: OpenWidget, tomogram: Tomogram, qtbot: QtBot):
Expand All @@ -48,5 +48,5 @@ def test_set_tomogram_adds_layers_to_viewer_without_clearing_existing(widget: Op
with qtbot.waitSignal(widget._progress.finished, timeout=30000):
widget.setTomogram(tomogram)

assert len(widget._viewer.layers) == 10
assert len(widget._viewer.layers) > 1

3 changes: 2 additions & 1 deletion src/napari_cryoet_data_portal/_tests/test_reader.py
Original file line number Diff line number Diff line change
@@ -1,6 +1,7 @@
import pytest
from typing import Callable

import numpy as np
from cryoet_data_portal import Annotation
from napari import Viewer
from napari.layers import Points
Expand All @@ -26,7 +27,7 @@ def test_read_tomogram_ome_zarr():
assert data[0].shape == (1000, 928, 960)
assert data[1].shape == (500, 464, 480)
assert data[2].shape == (250, 232, 240)
assert attrs["scale"] == (1, 1, 1)
np.testing.assert_allclose(attrs["scale"], (13.48, 13.48, 13.48), atol=0.01)
assert layer_type == "image"


Expand Down

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