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Corrected --writeMappings
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chilamakuricsreddy committed Mar 14, 2024
1 parent ff87193 commit b8660b6
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title: "Introduction to Bulk RNAseq data analysis"
subtitle: Quantification of Gene Expression with Salmon
output:
html_document: default
pdf_document: default
html_document: default
---

## Exercise 1 - Create Salmon index
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-1 fastq/SRR7657883.subset_2M.sra_1.fastq.gz \
-2 fastq/SRR7657883.subset_2M.sra_2.fastq.gz \
-o salmon_output/SRR7657883 \
--writeMappings salmon_output/SRR7657883/SRR7657883.salmon.sam \
--writeMappings=salmon_output/SRR7657883/SRR7657883.salmon.sam \
--gcBias \
-p 7
```
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Expand Up @@ -456,7 +456,7 @@ <h2>Exercise 2 - Quantify with Salmon</h2>
-1 fastq/SRR7657883.subset_2M.sra_1.fastq.gz \
-2 fastq/SRR7657883.subset_2M.sra_2.fastq.gz \
-o salmon_output/SRR7657883 \
--writeMappings salmon_output/SRR7657883/SRR7657883.salmon.sam \
--writeMappings=salmon_output/SRR7657883/SRR7657883.salmon.sam \
--gcBias \
-p 7</code></pre>
<div id="exercise-3" class="section level3">
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