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output methods generating alignment for each interval
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baoxingsong committed Aug 23, 2024
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-n FILE output anchors file
-o FILE output file in maf format
-f FILE output sequence alignment for each anchor/inter-anchor region in maf format
-b FILE output the sequence alignment method used for each anchor/inter-anchor region, in bed format
-t INT number of threads (default: 1)
-v FILE output variant calling in vcf format (conflict with -IV)
-m INT minimum exon length to use (default: 20, should be identical with the setting of gff2seq function)
-mi DOUBLE minimum full-length CDS anchor hit similarity to use (default:0.95)
-mi2 DOUBLE minimum novel anchor hit similarity to use (default:0.2)
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-n FILE output anchors file
-o FILE output file in maf format
-f FILE output sequence alignment for each anchor/inter-anchor region in maf format
-b FILE output the sequence alignment method used for each anchor/inter-anchor region, in bed format
-t INT number of threads (default: 1)
-fa3 INT if the inter-anchor length is shorter than this value, stop trying to find new anchors (default: 100000)
-w INT sequence alignment window width (default: 100000)
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This prevents using tandem duplicated genes to identify collinear block
-y DOUBLE minimal ratio of e+1 similarity to 1 similarity to drop an anchor (default: 0.6)
-ar FILE sam file by mapping conserved sequence to reference genome
this is used to improve the accurancy of anchors mapping
Following parameters are for identify collinear blocks
this is used to improve the accuracy of anchors mapping
Following parameters are to identify collinear blocks
-d DOUBLE calculate IndelDistance (default: 3)
-O DOUBLE chain open gap penalty (default: -0.03)
-E DOUBLE chain extend gap penalty (default: -0.01)
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