he MDMAPR 2.0 is an open-source and extensible Shiny web application that is able to merge raw qPCR fluorescence data and metadata together to facilitate the spatial visualization of species presence/absence detections. The application also has the ability to visualize qPCR fluorescence curves and standard curves to evaluate data quality. MDMAPR 2.0 aims to centralize varied qPCR data, which includes data from pathogen and environmental qPCR species detection studies, gene expression studies, and quantification studies used in identifying pathogen-associated health threats.
The MDMAPR 2.0 was developed by Alka Benawra and Abinaya Yogasekaram at the University of Guelph.
You can install the development version of MDMAPR2.0 like so:
install.packages("devtools")
devtools::install_github("ayogasekaram/MDMAPR2.0")Once installed, please load the package and run the following command to launch the MDMAPR App:
library(MDMAPR2.0)
launchMDMAPR()
The Mapping Dashboard is used to perform geospatial analysis on qPCR run samples. On this page, an interactive map displays location markers for qPCR run sample collection locations and allows for filtering of markers by Cq intensity, date, location, taxon details, machine type, project, and assay. Hovering above a certain marker will display a pop-up menu that shows information about the specific marker. As well, the page contains a data table which shows detailed information about the markers on the map. The data table will update based on the used filters. A copy of the data table can be downloaded as a CSV by pressing the ‘Download Mapped Markers Metadata’ button.
The Data Overview page is used to analyze individual tube/well samples and facilitates the quality control inspection of data. The page has four tabs, which include the ‘Presence/Absence Samples’, ‘Amplification Plot’, ‘Standard Curve Data Overview’, and ‘Standard Curve Plot’. The ‘Presence/Absence Samples’ tab displays a table which indicates if a target sequence was detected in a tube/well based on its Cq Value. The ‘Amplification Plot’ shows the amplification curve associated with a specific well sample from the ‘Presence/Absence Samples’ tab. The ‘Standard Curve Data Overview’ tab displays a table with information related to the standard curve used for the samples in the ‘Presence/Absence Samples’ tab. Lastly, the ‘Standard Curve Plot’ tab shows the plotted standard curve.
The Data Submission page is used to format raw qPCR fluorescence data and associated metadata into a format that is acceptable to be added to the MDMAPR 2.0 database for storage. On this page, a raw qPCR experimental fluorescence file, a raw standard curve fluorescence file, and the filled in MDMAPR 2.0 metadata file are required. The Data Submission tool will parse the data into 13 CSV files. A preview of the tables is viewable on the page. A zipped file of the CSVs can be downloaded by pressing the ‘Download Data Submission Files’ button. NOTE: Before you can upload the generated CSV data files into their respective tables in the MDMAPR 2.0 database, the ID columns (projectID, geographicRegionID, siteID, stationID, replicateID, extractID, assayID, runID, pcrChemistryID, resultID, standardCurveID, and SCresultID) must be manually changed from alphabetical characters to unique numeric IDs.