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rambaut committed Aug 23, 2024
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title: "Mpox virus sequencing | IT setup guide"
keywords: protocol
layout: document
last_updated: Jan 22, 2020
last_updated: Aug 20 2024
tags: [protocol]
permalink: # /mpxv/mpxv-it-setup.html
folder: mpxv
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{% include callout.html
type='default'
content='**Overview:** Instructions for setting up the laptop computing environment for running the MinION sequencer and performing the bioinformatics and downstream phylogenetic analyses.'
content='**Overview:** Instructions for setting up a computing environment for running the MinION sequencer and performing the bioinformatics and downstream phylogenetic analyses.'
%}

<br />

This document is part of the nCoV-2019 Nanopore sequencing protocol package:
: [http://artic.network/ncov-2019](http://artic.network/ncov-2019)
This document is part of the MPXV sequencing protocol package:
: [http://artic.network/mpxv](http://artic.network/ncov-2019)

#### Related documents:

nCoV-2019 Nanopore bioinformatics protocol:
: [http://artic.network/ncov-2019/ncov2019-bioinformatics-sop.html](http://artic.network/ncov-2019/ncov2019-bioinformatics-sop.html)
MPXV nanopore bioinformatics protocol:
: [http://artic.network/mpxv/mpxv-bioinformatics-sop.html](http://artic.network/mpxv/mpxv-bioinformatics-sop.html)

<br /><br />

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## Software Setup

https://labs.epi2me.io

https://labs.epi2me.io/downloads/

The current version of the EPI2ME Desktop application is v5.1.14

https://www.docker.com/products/docker-desktop/

These protocols instructions assume a 64-bit UNIX, Linux or similar environment. This could be Mac OS X (Yosemite or later), Linux (e.g., Ubuntu 16 or later), or Windows 10 Subsystem for Linux. It assumes familiarity with a UNIX-like *bash* command-line.

The steps in this document should be done and tested prior to sequencing, particularly if this will be done in an environment without internet access or where this is slow or unreliable. Once this is done, the bioinformatics and phylogenetics protocols can be performed largely off-line.
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