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@@ -9,73 +9,45 @@ The pipeline is configured using a YAML file: e.g. `config_atac.yml`, `config_ch | |
The following command will generate the working directory and configuration file for the ATAC-seq pipeline: | ||
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```bash | ||
seqnado-config atac | ||
seqnado-config chip | ||
``` | ||
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You should get somthing like this: | ||
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```bash | ||
$ seqnado-config chip | ||
[1/23] user_name (Your name): asmith | ||
[2/23] Select date | ||
1 - 2024-01-13 | ||
Choose from [1] (1): | ||
[3/23] project_name (Project name): TEST | ||
[4/23] Select project_id | ||
1 - test | ||
Choose from [1] (1): 1 | ||
[5/23] genome (hg38): | ||
[6/23] chromosome_sizes (/ceph/project/milne_group/shared/seqnado_reference/hg38/UCSC/sequence/hg38.chrom.sizes): | ||
[7/23] indices (/ceph/project/milne_group/shared/seqnado_reference/hg38/UCSC/bt2_index/hg38): | ||
[8/23] gtf (/ceph/project/milne_group/shared/seqnado_reference/hg38/UCSC/genes/hg38.ncbiRefSeq.gtf): | ||
[12/23] Select remove_pcr_duplicates_method | ||
1 - picard | ||
2 - deeptools | ||
Choose from [1/2] (1): 1 | ||
[13/23] Select remove_blacklist | ||
1 - yes | ||
2 - no | ||
Choose from [1/2] (1): 1 | ||
[14/23] blacklist (/ceph/project/milne_group/shared/seqnado_reference/hg38/hg38-blacklist.v2.bed.gz): | ||
[15/23] Select make_bigwigs | ||
1 - yes | ||
2 - no | ||
Choose from [1/2] (1): 1 | ||
[16/23] Select pileup_method | ||
1 - deeptools | ||
2 - homer | ||
Choose from [1/2] (1): 1 | ||
[17/23] Select make_heatmaps | ||
1 - yes | ||
2 - no | ||
Choose from [1/2] (1): 1 | ||
[18/23] Select call_peaks | ||
1 - yes | ||
2 - no | ||
Choose from [1/2] (1): 1 | ||
[19/23] Select peak_calling_method | ||
1 - macs | ||
2 - lanceotron | ||
3 - homer | ||
Choose from [1/2/3] (1): 2 | ||
[20/23] Select make_ucsc_hub | ||
1 - yes | ||
2 - no | ||
Choose from [1/2] (1): 1 | ||
[21/23] UCSC_hub_directory (path/to/ publically accessible location on the server): /project/milne_group/datashare/asmith/chipseq/TEST_HUB | ||
[22/23] email (Email address (UCSC required)): [email protected] | ||
[23/23] Select color_by | ||
1 - samplename | ||
2 - method | ||
Choose from [1/2] (1): 1 | ||
What is your project name? [cchahrou_project]: TEST | ||
What is your genome name? [other]: hg38 | ||
Path to Bowtie2 genome indices: [None]: /ceph/project/milne_group/shared/seqnado_reference/hg38/UCSC/bt2_index/hg38 | ||
Path to chromosome sizes file: [None]: /ceph/project/milne_group/shared/seqnado_reference/hg38/UCSC/sequence/hg38.chrom.sizes | ||
Path to GTF file: [None]: /ceph/project/milne_group/shared/seqnado_reference/hg38/UCSC/genes/hg38.ncbiRefSeq.gtf | ||
Path to blacklist bed file: [None]: /ceph/project/milne_group/shared/seqnado_reference/hg38/hg38-blacklist.v2.bed.gz | ||
Do you want to remove blacklist regions? (yes/no) [yes]: yes | ||
Remove PCR duplicates? (yes/no) [yes]: yes | ||
Remove PCR duplicates method: [picard]: picard | ||
Do you have spikein? (yes/no) [no]: yes | ||
Normalisation method: [orlando/with_input]: orlando | ||
Reference genome: [hg38]: hg38 | ||
Spikein genome: [dm6]: dm6 | ||
Path to fastqscreen config: [/ceph/project/milne_group/shared/seqnado_reference/fastqscreen_reference/fastq_screen.conf]: /ceph/project/milne_group/shared/seqnado_reference/fastqscreen_reference/fastq_screen.conf | ||
Do you want to make bigwigs? (yes/no) [no]: yes | ||
Pileup method: [deeptools/homer]: deeptools | ||
Do you want to make heatmaps? (yes/no) [no]: yes | ||
Do you want to call peaks? (yes/no) [no]: yes | ||
Peak caller: [lanceotron/macs/homer]: lanceotron | ||
Do you want to make a UCSC hub? (yes/no) [no]: yes | ||
UCSC hub directory: [/path/to/ucsc_hub/]: /project/milne_group/datashare/etc | ||
What is your email address? [[email protected]]: email for UCSC | ||
Color by (for UCSC hub): [samplename]: samplename | ||
Directory '2024-01-26_chip_TEST' has been created with the 'config_chip.yml' file. | ||
``` | ||
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This will generate the following files: | ||
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```bash | ||
$ tree 2024-01-13_test/ | ||
$ tree 2024-01-13_chip_test/ | ||
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2024-01-13_test/ | ||
2024-01-13_chip_test/ | ||
├── config_chip.yml | ||
└── readme_test.md | ||
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@@ -221,6 +193,13 @@ $ ls -l | |
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```bash | ||
tmux new -s NAME_OF_SESSION | ||
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# or | ||
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screen -S NAME_OF_SESSION | ||
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# to exit screen session | ||
ctrl+a d | ||
``` | ||
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