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# import glob | ||
# import os | ||
# import shutil | ||
# import subprocess | ||
# import pytest | ||
# from datetime import datetime | ||
# from cookiecutter.main import cookiecutter | ||
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# @pytest.fixture(scope="module") | ||
# def repo_path(): | ||
# path_file = os.path.abspath(__file__) | ||
# path_test = os.path.dirname(path_file) | ||
# path_repo = os.path.dirname(path_test) | ||
# return path_repo | ||
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# @pytest.fixture(scope="module") | ||
# def package_path(repo_path): | ||
# return os.path.join(repo_path, "seqnado") | ||
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# @pytest.fixture(scope="module") | ||
# def pipeline_path(package_path): | ||
# return os.path.join(package_path, "workflow", "snakefile_snp") | ||
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# @pytest.fixture(scope="module") | ||
# def test_dir_path(repo_path): | ||
# return os.path.join(repo_path, "tests") | ||
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# @pytest.fixture(scope="module") | ||
# def data_path(test_dir_path): | ||
# return os.path.join(test_dir_path, "data") | ||
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# @pytest.fixture(scope="module") | ||
# def genome_path(data_path): | ||
# return os.path.join(data_path, "genome") | ||
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# @pytest.fixture(scope="module") | ||
# def chromsizes(genome_path): | ||
# return os.path.join(genome_path, "chr21_rename.fa.fai") | ||
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# @pytest.fixture(scope="module") | ||
# def fastqs(data_path): | ||
# path = os.path.join(data_path, "fastq") | ||
# return glob.glob(os.path.join(path, "snp*.fastq.gz")) | ||
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# @pytest.fixture(scope="module") | ||
# def config_path(data_path): | ||
# return os.path.join(data_path, "config") | ||
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# @pytest.fixture(scope="module") | ||
# def genome_indicies(genome_path): | ||
# indicies = os.path.join(genome_path, "bt2") | ||
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# if not os.path.exists(indicies): | ||
# try: | ||
# import requests | ||
# import tarfile | ||
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# url = ( | ||
# "https://userweb.molbiol.ox.ac.uk/public/asmith/ngs_pipeline/bt2.tar.gz" | ||
# ) | ||
# output = os.path.join(genome_path, "bt2.tar.gz") | ||
# r = requests.get(url, stream=True) | ||
# with open(output, "wb") as f: | ||
# f.write(r.content) | ||
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# tar = tarfile.open(output) | ||
# tar.extractall(path=genome_path) | ||
# tar.close() | ||
# os.remove(output) | ||
# os.rename(genome_path + "/bt2", indicies) | ||
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# except Exception as e: | ||
# print(e) | ||
# print("Could not download indicies so generating them") | ||
# os.mkdir(indicies) | ||
# cmd = f"bowtie2-build {os.path.join(genome_path,'chr21_rename.fa')} {indicies}/bt2 --threads 8" | ||
# subprocess.run(cmd.split()) | ||
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# return os.path.join(indicies, "chr21") | ||
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# @pytest.fixture(scope="module") | ||
# def run_directory(tmpdir_factory): | ||
# fn = tmpdir_factory.mktemp("data") | ||
# return fn | ||
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# @pytest.fixture(scope="module", autouse=True) | ||
# def set_up( | ||
# run_directory, | ||
# data_path, | ||
# package_path, | ||
# repo_path, | ||
# genome_indicies, | ||
# chromsizes, | ||
# fastqs, | ||
# config_path, | ||
# ): | ||
# cwd = os.getcwd() | ||
# os.chdir(run_directory) | ||
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# cookiecutter( | ||
# f"{package_path}/workflow/config/cookiecutter_config/config_snp/", | ||
# extra_context={ | ||
# "genome": "hg19", | ||
# "date": "{% now 'utc', '%Y-%m-%d' %}", | ||
# "project_name": "test", | ||
# "chromosome_sizes": chromsizes, | ||
# "indicies": genome_indicies, | ||
# "design": "design.csv", | ||
# "read_type": "paired", | ||
# "split_fastq": "True", | ||
# "split_fastq_parts": "3", | ||
# "remove_pcr_duplicates_method": "picard", | ||
# "remove_blacklist": "yes", | ||
# "blacklist": f"{data_path}/genome/hg19-blacklist.v2.chr21.bed.gz", | ||
# "fasta": f"{data_path}/genome/chr21_rename.fa", | ||
# "fasta_index": f"{data_path}/genome/chr21_rename.fa.fai", | ||
# "call_snps": "yes", | ||
# "annotate_snps": "no", | ||
# "make_ucsc_hub": "no", | ||
# "UCSC_hub_directory": "test_hub", | ||
# "email": "test", | ||
# "color_by": "samplename", | ||
# }, | ||
# no_input=True, | ||
# ) | ||
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# # Move config files and fastq files | ||
# current_date = datetime.now().strftime("%Y-%m-%d") | ||
# os.chdir(f"{current_date}_test") | ||
# for fq in fastqs: | ||
# shutil.copy(fq, ".") | ||
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# yield | ||
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# os.chdir(cwd) | ||
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# def test_pipeline_singularity(genome_path): | ||
# indicies_dir = os.path.join(genome_path, "bt2") | ||
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# cmd = [ | ||
# "seqnado", | ||
# "snp", | ||
# "--cores", | ||
# "4", | ||
# "--configfile", | ||
# "config_snp.yml", | ||
# "--use-singularity", | ||
# "--singularity-args", | ||
# f'" -B {indicies_dir} -B {genome_path}"', | ||
# ] | ||
# completed = subprocess.run(" ".join(cmd), shell=True) | ||
# assert completed.returncode == 0 | ||
# assert not os.path.exists("seqnado_error.log") | ||
# assert os.path.exists("seqnado_output/") |