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--outFilterType BySJout //reduces the number of "spurious" junctions | ||
--outFilterMultimapNmax 20 //max number of multiple alignments allowed for a read: if exceeded, the read is considered unmapped | ||
--alignSJoverhangMin 8 //min overhang for unannotated junctions | ||
--alignSJDBoverhangMin 1 //min overhang for annotated junctions | ||
--outFilterMismatchNmax 999 //max number of mismatches per pair (absolute) | ||
--outFilterMismatchNoverLmax 0.06 //max number of mismatches per pair relative to read length: for 2x100b, max number of mismatches is 0.06*200=12 for the paired read | ||
--alignIntronMin 20 //min intron | ||
--alignIntronMax 1000000 //max intron | ||
--alignMatesGapMax 1000000 //max genomic distance between pairs |
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