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2.7.8a
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alexdobin committed Feb 20, 2021
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9 changes: 9 additions & 0 deletions docs/STARsolo.md
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Expand Up @@ -199,6 +199,15 @@ CellRanger 3.0.0 use advanced filtering based on the EmptyDrop algorithm develop
```
It can be followed by 10 numeric parameters: nExpectedCells (3000), maxPercentile (0.99), maxMinRatio (10), indMin (45000), indMax (90000), umiMin (500), umiMinFracMedian (0.01), candMaxN (20000), FDR (0.01), simN (10000).
#### Cell filtering of previously generated raw matrix
It is possible to run only the filtering algorithm (without the need to re-map) inputting the previously generated **raw** matrix:
```
STAR --runMode soloCellFiltering /path/to/count/dir/raw/ /path/to/output/prefix --soloCellFilter EmptyDrops_CR
```
The */path/to/count/dir/raw/* directory should contain the **"raw"** *barcodes.tsv*, *features.tsv*, and *matrix.mtx* files generated in a previos STARsolo run.
The output will contain the filtered files.
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Quantification of different transcriptomic features
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