Virtual-2D-protein-gel

Get Virtual 2D Gel on http://www.jvirgel.de/ automatically

1. Download protein fasta files form NCBI
   Excute "Download_Protein_from_NCBI.exe"
   Input protein's accession number and will find it on NCBI (Database: Protein)

   Search from NCBI, database:Protein
   
   #1 Input Accession: EPE32547.1
   Searching EPE32547.1......
   Save as EPE32547.1.fas
   
   #2 Input Accession: NP_001162128.1
   Searching NP_001162128.1......
   Save as NP_001162128.1.fas
   
   #3 Input Accession: gslgnbsldkgn
   Searching gslgnbsldkgn......
   ERROR: Can't find gslgnbsldkgn
   

   In this case, Download_Protein_from_NCBI.exe will only generate 3 files,
   EPE32547.1.fas,
   NP_001162128.1.fas,
   and All_fas.fas file, this is for 2Dgel.exe in next step.

2. Get virtual 2D protein gel
   Results were generated by http://www.jvirgel.de/

   2-1. Make sure All_fas.fas exist. Generate All_fas.fas file by step 1 automatically, or rename your own file.

    Excute "Download_Protein_from_NCBI.exe" first, or name fasta file as "All_fas.fas"
   

   This error message shows when All_fas.fas doesn't exist.

   2-2. Input the path way of "chromedrive.exe"
   If chromedrive.exe was located in C:\User\user\Desktop

    Input chromedriver's path: C:\User\user\Desktop
   

   2-3. Get the picture of 2D gel, and MW and pI.
   This step will finish automatically.

    DevTools listening on ws://127.0.0.1:49738/devtools/browser/322c984d-6518-4c1c-8a0f-0c104eb55a6a
    Paste 2 sequences
    Choose: No signal peptide correction (not recommended)
    Choose: HTML-Version
               
    Save picture as '2dgel.png'
      
    Get results:
    Name                    MW      pi
    "EPE32547.1"...         91.66   9.74
    "NP_001162128.1"...     22.43   8.82
       
    Save results as "2Dgel_result.txt"
    === Finish ===
   

3. Path of Output files All files will output to "output" folder.