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update README
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SwiftSeal committed Jan 6, 2025
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![Conda Downloads](https://img.shields.io/conda/dn/bioconda/resistify)
[![Open In Colab](https://colab.research.google.com/assets/colab-badge.svg)](https://colab.research.google.com/github/swiftseal/resistify/blob/main/assets/resistify.ipynb)

*More than 2,500 downloads - thank you all!*
</div>

Resistify is a program which rapidly identifies and classifies plant resistance genes from protein sequences.
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If you are having issues with `conda`, you can instead try installing directly from the repository:

```sh
pip install https://github.com/SwiftSeal/resistify/archive/refs/tags/v0.6.2.tar.gz
pip install https://github.com/SwiftSeal/resistify/archive/refs/tags/v1.0.1.tar.gz
```

Note that `resistify` requires `hmmer` to be installed and available in your system's PATH, which will not be installed automatically when using `pip`.
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### results.tsv (nlr)

| Sequence | Length | Motifs | Domains | Classification | NBARC_motifs | MADA | MADAL | CJID |
| --- | --- | --- | --- | --- | --- | --- | --- | --- |
| ZAR1 | 852 | CNNNNNNNNNLLLLLLLLLL | mCNL | CNL | 9 | False | True | False |
| Sequence | Length | LRR_Length | Motifs | Domains | Classification | NBARC_motifs | MADA | MADAL | CJID |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| ZAR1 | 852 | 307 | CNNNNNNNNNLLLLLLLLLL | mCNL | CNL | 9 | False | True | False |

The main column of interest is "Classification", where we can see that it has been identified as a canonical CNL.
The "Motifs" column indicates the series of NLR-associated motifs identified across the sequence - this can be useful if an NLR has an undetermined or unexpected classification.
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### results.tsv (prr)

| Sequence | Length | Type | Classification | Signal_peptide |
| --- | --- | --- | --- | --- |
| fls2 | 1174 | RLK | LRR | True |
| Sequence | Length | Extracellular_Length | LRR_Length | Type | Classification | Signal_peptide |
| --- | --- | --- | --- | --- | --- | --- |
| fls2 | 1173 | 806 | 675 | RLK | LRR | True |

For PRRs, sequences can be of the type RLP or RLK - both are single pass transmembrane proteins, and RLKs have an internal kinase domain.
Classification refers to the domains identified in the external region.
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