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Update AVITI run stats parser to handle multiple lanes #360

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Sep 12, 2024
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4 changes: 4 additions & 0 deletions VERSIONLOG.md
Original file line number Diff line number Diff line change
@@ -1,5 +1,9 @@
# Scilifelab_epps Version Log

## 20240912.1

Update AVITI run stats parser to handle multiple lanes

## 20240910.5

Fix simple naming bug.
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45 changes: 26 additions & 19 deletions scripts/aviti_run_parameter_parser.py
Original file line number Diff line number Diff line change
Expand Up @@ -55,7 +55,6 @@ def attach_json_files(process, run_dir):
lims.upload_new_file(outart, f"{run_dir}/AvitiRunStats.json")
except OSError:
sys.stderr.write("No AvitiRunStats.json found")
sys.exit(2)


def parse_run_parameters(run_dir):
Expand Down Expand Up @@ -158,25 +157,33 @@ def calculate_mean(input_list, key):


def set_run_stats(process, run_dir):
global lane_stats
run_stats = parse_run_stats(run_dir)
art = process.input_output_maps[0][0]["uri"]

for read in run_stats["RunStats"]["Reads"]:
read_key = read["Read"]
art[f"Reads PF (M) {read_key}"] = run_stats["RunStats"]["PFCount"] / 1000000
art[f"%PF {read_key}"] = run_stats["RunStats"]["PercentPF"]
art[f"Yield PF (Gb) {read_key}"] = (
run_stats["RunStats"]["TotalYield"] / 1000000000
)
art[f"% Aligned {read_key}"] = read["PhiXAlignmentRate"]
art[f"% Bases >=Q30 {read_key}"] = calculate_mean(read["Cycles"], "PercentQ30")
art[f"% Bases >=Q40 {read_key}"] = calculate_mean(read["Cycles"], "PercentQ40")
art[f"Avg Q Score {read_key}"] = calculate_mean(read["Cycles"], "AverageQScore")
art[f"% Error Rate {read_key}"] = calculate_mean(
read["Cycles"], "PercentPhixErrorRate"
)

art.put()
for art in process.all_outputs():
if "Lane" in art.name:
lane_nbr = int(art.name.split(" ")[1])
lane_stats = next(
d for d in run_stats["LaneStats"] if d["Lane"] == lane_nbr
)
for read in lane_stats["Reads"]:
read_key = read["Read"]
art.udf[f"Reads PF (M) {read_key}"] = lane_stats["PFCount"] / 1000000
art.udf[f"%PF {read_key}"] = lane_stats["PercentPF"]
art.udf[f"Yield PF (Gb) {read_key}"] = lane_stats["TotalYield"] / 1000000000
art.udf[f"% Aligned {read_key}"] = read["PhiXAlignmentRate"]
art.udf[f"% Bases >=Q30 {read_key}"] = calculate_mean(
read["Cycles"], "PercentQ30"
)
art.udf[f"% Bases >=Q40 {read_key}"] = calculate_mean(
read["Cycles"], "PercentQ40"
)
art.udf[f"Avg Q Score {read_key}"] = calculate_mean(
read["Cycles"], "AverageQScore"
)
art.udf[f"% Error Rate {read_key}"] = calculate_mean(
read["Cycles"], "PercentPhixErrorRate"
)
art.put()
process.put()


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