This code will analyse flourescence microscopy images of cells transfected with flourescently tagged proteins with different localizations. It will output a classic boxplot with scatter for each datapoint and calculate statistical significance for each condition via t-test if the data is normally distributed.
- Brightfield image
- Signal localized in the cytoplasm
- Signal localized in the nucleus
The program assumes the data is in a specific folder and different channels are present as their own unique files. All files should be named starting with an increasing number for each condition.
Folder, file extentions and sample names have to be enterted inside the code!, below the if __name__ == "__main__"