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Merge branch 'master' into refactor/docs-exec
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alethomas authored Nov 5, 2024
2 parents 68ed1ed + 17b4f32 commit 19d21ba
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2 changes: 1 addition & 1 deletion .github/workflows/check-todos.yml
Original file line number Diff line number Diff line change
Expand Up @@ -11,4 +11,4 @@ jobs:
steps:
- uses: "actions/checkout@master"
- name: "TODO to Issue"
uses: "alstr/todo-to-issue-action@v4.13"
uses: "alstr/todo-to-issue-action@v5"
2 changes: 1 addition & 1 deletion .github/workflows/copyright-preamble.yml
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Expand Up @@ -25,7 +25,7 @@ jobs:

# Creates pull request with all changes in file
- name: Create Pull Request
uses: peter-evans/create-pull-request@v6
uses: peter-evans/create-pull-request@v7
with:
commit-message: "chore: update copyright preamble"
delete-branch: true
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9 changes: 4 additions & 5 deletions .github/workflows/main.yml
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Expand Up @@ -23,8 +23,10 @@ jobs:
runs-on: ubuntu-latest
steps:
- uses: actions/checkout@v4
with:
fetch-depth: 0
- name: Formatting
uses: super-linter/super-linter@v5.7.2
uses: super-linter/super-linter@v7.1.0
env:
VALIDATE_ALL_CODEBASE: false
DEFAULT_BRANCH: master
Expand Down Expand Up @@ -64,7 +66,6 @@ jobs:
env:
GISAID_API_TOKEN: ${{ secrets.GISAID_API_TOKEN }}
needs:
- Formatting
- Linting
#- Pre-Commit
strategy:
Expand Down Expand Up @@ -179,7 +180,7 @@ jobs:
uses: actions/upload-artifact@v4
if: matrix.technology == 'all' && matrix.rule != 'all -npr'
with:
name: log-rule-${{ matrix.rule }}-technology-${{ matrix.technology }}
name: log-rule-${{ matrix.rule }}-technology-${{ matrix.technology }}-${{ matrix.seq_method }}
path: .tests/logs/

- name: Change permissions for caching
Expand All @@ -193,7 +194,6 @@ jobs:
env:
GISAID_API_TOKEN: ${{ secrets.GISAID_API_TOKEN }}
needs:
- Formatting
- Linting
#- Pre-Commit
strategy:
Expand All @@ -203,7 +203,6 @@ jobs:
benchmark_strain_calling,
benchmark_assembly,
benchmark_mixtures,
benchmark_non_sars_cov_2,
benchmark_reads,
compare_assemblers,
]
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2 changes: 1 addition & 1 deletion .github/workflows/release-please.yml
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Expand Up @@ -140,7 +140,7 @@ jobs:

- name: Build and push
if: ${{ steps.release.outputs.release_created == 'true' }}
uses: docker/build-push-action@v5
uses: docker/build-push-action@v6
with:
context: .
push: true
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2 changes: 1 addition & 1 deletion .github/workflows/update-pangolin.yaml
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Expand Up @@ -37,7 +37,7 @@ jobs:
- name: Create Pull Request
if: ${{ env.LATEST_PANGOLIN != env.CURRENT_PANGOLIN }}
id: cpr
uses: peter-evans/create-pull-request@v6
uses: peter-evans/create-pull-request@v7
with:
token: ${{ secrets.GITHUB_TOKEN }}
committer: GitHub <[email protected]>
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22 changes: 22 additions & 0 deletions CHANGELOG.md
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@@ -1,5 +1,27 @@
# Changelog

## [1.1.1](https://github.com/IKIM-Essen/uncovar/compare/v1.1.0...v1.1.1) (2024-11-04)


### Bug Fixes

* fix file not found error on shared fs storage ([#673](https://github.com/IKIM-Essen/uncovar/issues/673)) ([c28db96](https://github.com/IKIM-Essen/uncovar/commit/c28db9608c816cc775397096d45bd33c8e59aace))

## [1.1.0](https://github.com/IKIM-Essen/uncovar/compare/v1.0.0...v1.1.0) (2024-10-31)


### Features

* FLiRT Mutations column ([#664](https://github.com/IKIM-Essen/uncovar/issues/664)) ([f77d42e](https://github.com/IKIM-Essen/uncovar/commit/f77d42e2154a95b6ca0e2c127df8ca134eda1789))


### Bug Fixes

* changed kraken input files for ont ([#650](https://github.com/IKIM-Essen/uncovar/issues/650)) ([b62007c](https://github.com/IKIM-Essen/uncovar/commit/b62007c7b10b9d933528a6b02933cca4bd751100))
* move primer file location under workflow; this avoids file not being found in shared filesystems ([#665](https://github.com/IKIM-Essen/uncovar/issues/665)) ([9cb1576](https://github.com/IKIM-Essen/uncovar/commit/9cb1576a8c41c23344940f2248e5d44464a53b2e))
* pangolin data updates ([#662](https://github.com/IKIM-Essen/uncovar/issues/662)) ([f46ec83](https://github.com/IKIM-Essen/uncovar/commit/f46ec833ed56c39820c0a0e8d8e0295050a334ab))
* replace ragoo with ragtag ([#591](https://github.com/IKIM-Essen/uncovar/issues/591)) ([e64e9db](https://github.com/IKIM-Essen/uncovar/commit/e64e9db7e81a295ff1775b880c255b71274448fd))

## [1.0.0](https://github.com/IKIM-Essen/uncovar/compare/v0.16.0...v1.0.0) (2024-04-17)


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2 changes: 1 addition & 1 deletion version.txt
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@@ -1 +1 @@
1.0.0
1.1.1
2 changes: 1 addition & 1 deletion workflow/Snakefile
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Expand Up @@ -21,7 +21,7 @@ report: "report/workflow.rst"
container: "docker://condaforge/mambaforge"


containerized: "quay.io/uncovar/uncovar#1.0.0"
containerized: "quay.io/uncovar/uncovar#1.1.1"


include: "rules/common.smk"
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1 change: 1 addition & 0 deletions workflow/envs/pangolin.yaml
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Expand Up @@ -4,3 +4,4 @@ channels:
- nodefaults
dependencies:
- pangolin =4.3
- snakemake-minimal=7.24.0 # TODO keep until https://github.com/bioconda/bioconda-recipes/pull/42192 and we can pin to 4.3.1 above
2 changes: 1 addition & 1 deletion workflow/envs/ragoo.yaml → workflow/envs/ragtag.yaml
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Expand Up @@ -4,4 +4,4 @@ channels:
- imperial-college-research-computing
- nodefaults
dependencies:
- ragoo =1.1
- ragtag =2.1
2 changes: 1 addition & 1 deletion workflow/envs/spades.yaml
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Expand Up @@ -3,4 +3,4 @@ channels:
- bioconda
- nodefaults
dependencies:
- spades =3.15
- spades =4.0
2 changes: 1 addition & 1 deletion workflow/report/assembly_illumina.rst
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@@ -1,3 +1,3 @@
Assembly of sample {{ snakemake.wildcards.sample }}.
Reads were assembled with {{ "`metaSPAdes <https://github.com/ablab/spades>`_" if snakemake.params.is_amp else "`Megahit <https://github.com/voutcn/megahit>`_" }}, followed by reference based contig ordering and concatenation with `RaGOO <https://github.com/malonge/RaGOO>`_.
Reads were assembled with {{ "`metaSPAdes <https://github.com/ablab/spades>`_" if snakemake.params.is_amp else "`Megahit <https://github.com/voutcn/megahit>`_" }}, followed by reference based contig ordering and concatenation with `RagTag <https://github.com/malonge/RagTag>`_.
Then, assembly was polished by applying variants with an allele frequency of 100% (called by `Varlociraptor <https://varlociraptor.github.io>`_ at FDR 5%).
2 changes: 1 addition & 1 deletion workflow/report/assembly_ont.rst
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@@ -1,2 +1,2 @@
Assembly of sample {{ snakemake.wildcards.sample }}.
Reads were assembled with `SPAdes <https://github.com/ablab/spades>`_, followed by reference based contig ordering and concatenation with `RaGOO <https://github.com/malonge/RaGOO>`_.
Reads were assembled with `SPAdes <https://github.com/ablab/spades>`_, followed by reference based contig ordering and concatenation with `RagTag <https://github.com/malonge/RagTag>`_.
2 changes: 1 addition & 1 deletion workflow/report/qc-report.rst
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@@ -1,3 +1,3 @@
QC overview for samples from {{ snakemake.wildcards.date }}.
Readcounts for raw, trimmed and filtered reads, length of initially assembled (`Megahit <https://github.com/voutcn/megahit>`_/`metaSPAdes <https://github.com/ablab/spades>`_) and reference ordered contigs (`RaGOO <https://github.com/malonge/RaGOO>`_).
Readcounts for raw, trimmed and filtered reads, length of initially assembled (`Megahit <https://github.com/voutcn/megahit>`_/`metaSPAdes <https://github.com/ablab/spades>`_) and reference ordered contigs (`RagTag <https://github.com/malonge/RagTag>`_).
Percentil overview for contamination in the raw reads (`kraken <https://github.com/DerrickWood/kraken>`_) and called COVID-19-strain including defining SNPs (`Pangolin <https://github.com/cov-lineages/pangolin>`_) and variants of interest from variant calling (`Varlociraptor <https://varlociraptor.github.io>`_).
218 changes: 218 additions & 0 deletions workflow/resources/nCoV-2019.primer.bed
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MN908947.3 17966 17993 nCoV-2019_60_LEFT 2 +
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MN908947.3 24978 25003 nCoV-2019_83_LEFT 1 +
MN908947.3 25347 25369 nCoV-2019_83_RIGHT 1 -
MN908947.3 25279 25301 nCoV-2019_84_LEFT 2 +
MN908947.3 25646 25673 nCoV-2019_84_RIGHT 2 -
MN908947.3 25601 25623 nCoV-2019_85_LEFT 1 +
MN908947.3 25969 25994 nCoV-2019_85_RIGHT 1 -
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MN908947.3 26835 26857 nCoV-2019_89_LEFT 1 +
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MN908947.3 28081 28104 nCoV-2019_93_LEFT 1 +
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MN908947.3 29836 29866 nCoV-2019_98_RIGHT 2 -
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