Skip to content

Commit

Permalink
- make AnalysisSummary semantic rule clearer in terms of SRM
Browse files Browse the repository at this point in the history
Former-commit-id: d785d413a04b8c2dce8aa7cc2a8da145ecae944d
  • Loading branch information
[email protected] committed Mar 19, 2015
1 parent 4e19ee3 commit e01cff5
Show file tree
Hide file tree
Showing 2 changed files with 10 additions and 5 deletions.
4 changes: 2 additions & 2 deletions schema/Schema_rules_absolute_quant_under_review.txt
Original file line number Diff line number Diff line change
Expand Up @@ -13,11 +13,11 @@ The following term MAY also be used if an SRM analysis has been performed in lab

MS:1001838 - name: SRM quantitation analysis

The following MUST both be present in AnalysisSummary with values of true of false :
The following MUST both be present in AnalysisSummary with values of true or false :

MS:XXXXXXX: "peptide internal reference used" (if true, an AssayQuantLayer of "internal reference abundance" MUST be present in PeptideConsensusList)
MS:XXXXXXX: "protein internal reference used" (if true, an AssayQuantLayer of "internal reference abundance" MUST be present in ProteinList)

- If either value of CV term "peptide internal reference used" or "protein internal reference used" is true, there MUST be a AssayQuantLayer in PeptideConsensusList or ProteinList respectively, of data type -- "internal reference abundance", with appropriate unit.
- If either value of CV term "peptide internal reference used" or "protein internal reference used" is true, there MUST be an AssayQuantLayer in PeptideConsensusList or ProteinList respectively, of data type -- "internal reference abundance", with appropriate unit.

- There SHOULD be at least one peptide-level, protein-level or protein group-level AssayQuantLayer containing absolute abundance values for all peptides or proteins.
11 changes: 8 additions & 3 deletions schema/Schema_rules_general.txt
Original file line number Diff line number Diff line change
Expand Up @@ -19,11 +19,16 @@ Failure of a rule with a SHOULD causes a WARNING to be produced

Mapping file additions and associated rules
*********************************************
- There MUST be ONE or MORE cvParams in AnalysisSummary for the general type of technique. We support four techniques in level 1 release (add following cvParams where they do not already exist):
"MS2 Tag-based analysis" (General term needed? Otherwise we could just use specific terms for iTRAQ / TMT etc?)
"MS1 Label-based analysis" (General term needed? Otherwise we could just use specific terms for SILAC / N15 etc?)
- There MUST be ONE or MORE cvParams in AnalysisSummary for the general type of technique. We support five techniques in level 1 release:
id: MS:1002023 - name: MS2 Tag-based analysis
id: MS:1002018 - name: MS1 Label-based analysis
id: MS:1001834 - name: LC-MS label-free quantitation analysis
id: MS:1001836 - name: spectral counting quantitation analysis
id: MS:1001838 - name: SRM quantitation analysis

- If "SRM quantitation analysis" is present in <AnlaysisSummary>, either "LC-MS label-free quantitation analysis" or "MS1 Label-based analysis" MUST be present in <AnlaysisSummary>.

- If "SRM quantitation analysis" is not present in <AnlaysisSummary>, EXACTLY ONE of the other four CV terms MUST be present in <AnalysisSummary>


Additional notes or discussed rules not currently to implement
Expand Down

0 comments on commit e01cff5

Please sign in to comment.