R Bindings for htslib/bcf+vcf
Requirements: R, git, curl-dev,
git clone "https://github.com/lindenb/rbcf"
cd rbcf
make
Pierre Lindenbaum PhD. Institut du Thorax. Nantes, France. @yokofakun
Code:
# load the library
library(rbcf)
#print the version of the associated htslib
paste("HTSLIB:",htslib.version())
#print the version of rbcf
paste("RBCF:",rcbf.version())
Output:
[1] "HTSLIB: 1.10.2"
[1] "RBCF: 0.0-1"
Code:
# load rbcf
library(rbcf)
# we don't need the index for this file
fp <- bcf.open("./data/rotavirus_rf.01.vcf",FALSE)
# error (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# dispose the vcf reader
bcf.close(fp)
print("Done.")
Output:
[1] TRUE
[1] "Done."
Code:
# load rbcf
library(rbcf)
# we don't need the index for this file
fp <- bcf.open("./data/rotavirus_rf.01.vcf",FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# print INFO
bcf.infos(fp)
# dispose the vcf reader
bcf.close(fp)
# print the table
Output:
ID Number Type
INDEL INDEL 0 Flag
IDV IDV 1 Integer
IMF IMF 1 Float
DP DP 1 Integer
VDB VDB 1 Float
RPB RPB 1 Float
MQB MQB 1 Float
BQB BQB 1 Float
MQSB MQSB 1 Float
SGB SGB 1 Float
MQ0F MQ0F 1 Float
ICB ICB 1 Float
HOB HOB 1 Float
AC AC A Integer
AN AN 1 Integer
DP4 DP4 4 Integer
MQ MQ 1 Integer
Descri(...)
INDEL "Indicates that the variant is an IN(...)
IDV "Maximum number of reads supporting an i(...)
IMF "Maximum fraction of reads supporting an i(...)
DP "Raw read d(...)
VDB "Variant Distance Bias for filtering splice-site artefacts in RNA-seq data (bigger is bet(...)
RPB "Mann-Whitney U test of Read Position Bias (bigger is bet(...)
MQB "Mann-Whitney U test of Mapping Quality Bias (bigger is bet(...)
BQB "Mann-Whitney U test of Base Quality Bias (bigger is bet(...)
MQSB "Mann-Whitney U test of Mapping Quality vs Strand Bias (bigger is bet(...)
SGB "Segregation based met(...)
MQ0F "Fraction of MQ0 reads (smaller is bet(...)
ICB "Inbreeding Coefficient Binomial test (bigger is bet(...)
HOB "Bias in the number of HOMs number (smaller is bet(...)
AC "Allele count in genotypes for each ALT allele, in the same order as li(...)
AN "Total number of alleles in called genot(...)
DP4 "Number of high-quality ref-forward , ref-reverse, alt-forward and alt-reverse b(...)
MQ "Average mapping qua(...)
[1] TRUE
Code:
# load rbcf
library(rbcf)
# we don't need the index for this file
fp <- bcf.open("./data/rotavirus_rf.01.vcf",FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# print FORMAT
bcf.formats(fp)
# dispose the vcf reader
bcf.close(fp)
Output:
ID Number Type Description
PL PL G Integer "List of Phred-scaled genotype likelihoods"
GT GT 1 String "Genotype"
[1] TRUE
Code:
# load rbcf
library(rbcf)
# we don't need the index for this file
fp <- bcf.open("./data/gnomad.exomes.r2.0.1.sites.bcf",FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# print FILTERs
bcf.filters(fp)
# dispose the vcf reader
bcf.close(fp)
Output:
ID
PASS PASS
AC0 AC0
InbreedingCoeff InbreedingCoeff
LCR LCR
RF RF
SEGDUP SEGDUP
(...)
PASS (...)
AC0 "Allele Count is zero (i.e. no high-confidence genotype (GQ >= 20, DP >= 10, AB(...)
InbreedingCoeff (...)
LCR "In (...)
RF "Failed random forests filters (SNV cutoff (...)
SEGDUP "In a segme(...)
[1] TRUE
The samples are defined in the '#CHROM' line of the VCF
Code:
# load rbcf
library(rbcf)
# we don't need the index for this file
fp <- bcf.open("./data/rotavirus_rf.01.vcf",FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# print the number of samples
paste("Number of samples:",bcf.nsamples(fp))
# get the name for the 1st sample
paste("First sample:",bcf.sample.at(fp,1))
# get the 1-based index for the samples
bcf.sample2index(fp,c("S1","S2","S3","missing"))
# get all the samples
bcf.samples(fp)
# dispose the vcf reader
bcf.close(fp)
Output:
[1] "Number of samples: 5"
[1] "First sample: S1"
S1 S2 S3 missing
1 2 3 0
[1] "S1" "S2" "S3" "S4" "S5"
[1] TRUE
Code:
# load rbcf
library(rbcf)
# we don't need the index for this file
fp <- bcf.open("./data/rotavirus_rf.01.vcf",FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# print the dictionary
bcf.dictionary(fp)
# dispose the vcf reader
bcf.close(fp)
Output:
chrom size
RF01 RF01 3302
RF02 RF02 2687
RF03 RF03 2592
RF04 RF04 2362
RF05 RF05 1579
RF06 RF06 1356
RF07 RF07 1074
RF08 RF08 1059
RF09 RF09 1062
RF10 RF10 751
RF11 RF11 666
[1] TRUE
Code:
# load rbcf
library(rbcf)
# Open the indexed VCF
fp <- bcf.open("./data/rotavirus_rf.02.vcf.gz")
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# get the indexed contigs
bcf.contigs(fp)
# dispose the vcf reader
bcf.close(fp)
Output:
[1] "RF01" "RF02" "RF03" "RF04" "RF05" "RF06" "RF07" "RF08" "RF09" "RF10"
[11] "RF11"
[1] TRUE
Code:
# load rbcf
library(rbcf)
# create a function counting variants in a VCF
count.variants<-function(filename) {
# we don't need the index for this file
fp <- bcf.open(filename,FALSE)
# error on opening
if(is.null(fp)) return(-1)
# number of variants
n<-0
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
# increment the count
n<-n+1
}
# dispose the vcf reader
bcf.close(fp)
# return the number of variant
n
}
# filenames
vcfs<-c(
"./data/gnomad.exomes.r2.0.1.sites.bcf",
"./data/rotavirus_rf.01.vcf",
"./data/rotavirus_rf.02.vcf.gz",
"./data/rotavirus_rf.03.vcf.gz",
"./data/rotavirus_rf.04.bcf"
)
# print the number of variants for each vcf
for(f in vcfs) {
cat(paste(f," ",count.variants(f),"\n"))
}
Output:
./data/gnomad.exomes.r2.0.1.sites.bcf 50
./data/rotavirus_rf.01.vcf 45
./data/rotavirus_rf.02.vcf.gz 45
./data/rotavirus_rf.03.vcf.gz 45
./data/rotavirus_rf.04.bcf 45
Code:
# load rbcf
library(rbcf)
# create a function counting variants in a VCF
count.variants<-function(filename,predicate) {
# we don't need the index for this file
fp <- bcf.open(filename,FALSE)
# error on opening
if(is.null(fp)) return(-1)
# number of variants
n<-0
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
# test the variant
if(predicate(vc)) {
# increment the count
n<-n+1
}
}
# dispose the vcf reader
bcf.close(fp)
# return the number of variant
n
}
# A vcf
filename <- "./data/gnomad.exomes.r2.0.1.sites.bcf"
# filters
filters<-list(
list("desc"="accept all","predicate"=function(ctx) {TRUE} ),
list("desc"="accept none","predicate"=function(ctx) {FALSE} ),
list("desc"="CHROM is '1'","predicate"=function(ctx) { variant.contig(ctx)=="1"} ),
list("desc"="POS is even","predicate"=function(ctx) { (variant.pos(ctx)%%2)==1} ),
list("desc"="PASS filter","predicate"=function(ctx) {!variant.is.filtered(ctx)} ),
list("desc"="count(FILTER)>1","predicate"=function(ctx) {length(variant.filters(ctx))>1} ),
list("desc"="FILTER contains SEGDUP","predicate"=function(ctx) {variant.has.filter(ctx,"SEGDUP")} ),
list("desc"="SNP","predicate"=function(ctx) {variant.is.snp(ctx)} ),
list("desc"="POS!=END","predicate"=function(ctx) { variant.pos(ctx)!=variant.end(ctx)} ),
list("desc"="not diallelic","predicate"=function(ctx) {variant.nalleles(ctx)!=2} ),
list("desc"="REF is 'A'","predicate"=function(ctx) {variant.reference(ctx)=="A"} ),
list("desc"="any allele is 'A'","predicate"=function(ctx) {"A" %in% variant.alleles(ctx)} ),
list("desc"="any ALT allele is 'A'","predicate"=function(ctx) {"A" %in% variant.alt.alleles(ctx)} ),
list("desc"="No QUAL","predicate"=function(ctx) {!variant.has.qual(ctx)} ),
list("desc"="variant has ID","predicate"=function(ctx) {variant.has.id(ctx)}),
list("desc"="variant ID match 'rs1*' ","predicate"=function(ctx) {grepl("^rs1",variant.id(ctx))}),
list("desc"="variant has INFO/AF_NFE","predicate"=function(ctx) {variant.has.attribute(ctx,"AF_NFE")}),
list("desc"="variant has INFO/AF_NFE > 1E-5","predicate"=function(ctx) {variant.has.attribute(ctx,"AF_NFE") && length(which(variant.float.attribute(ctx,"AF_NFE") > 1E-5))>0}),
list("desc"="Missense in PLEKHN1 (VEP)","predicate"=function(ctx) {
# NO VEP annotation ?
if(!variant.has.attribute(ctx,"CSQ")) return(FALSE);
# get VEP annotation
predictions <- variant.vep(ctx)
# In SCN5A
predictions <- predictions[which(predictions$SYMBOL=="PLEKHN1"),]
# Consequence must contain missense
predictions <- predictions[grep("missense_variant",predictions$Consequence),]
nrow(predictions)>0
})
)
# count the variant for each filter
for(flt in filters) {
print(paste(basename(filename)," filter:",flt[["desc"]]," count:",count.variants(filename,flt[["predicate"]]),"\n"))
}
Output:
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: accept all count: 50 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: accept none count: 0 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: CHROM is '1' count: 50 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: POS is even count: 24 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: PASS filter count: 48 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: count(FILTER)>1 count: 2 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: FILTER contains SEGDUP count: 1 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: SNP count: 47 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: POS!=END count: 3 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: not diallelic count: 8 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: REF is 'A' count: 6 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: any allele is 'A' count: 27 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: any ALT allele is 'A' count: 21 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: No QUAL count: 1 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: variant has ID count: 34 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: variant ID match 'rs1*' count: 2 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: variant has INFO/AF_NFE count: 50 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: variant has INFO/AF_NFE > 1E-5 count: 14 \n"
[1] "gnomad.exomes.r2.0.1.sites.bcf filter: Missense in PLEKHN1 (VEP) count: 13 \n"
Code:
# load rbcf
library(rbcf)
# A vcf
filename <- "./data/gnomad.exomes.r2.0.1.sites.bcf"
# we don't need the index for this file
fp <- bcf.open(filename,FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# current variant
vc <- NULL
while(!is.null(vc<-bcf.next(fp))) {
#find the first variant having an INFO/CSQ attribute
if(variant.has.attribute(vc,"CSQ")) break;
}
if(!is.null(vc)) {
# get the VEP table for the variant
predictions<-variant.vep(vc)
}
# dispose the vcf reader
bcf.close(fp)
# show
predictions
Output:
[1] TRUE
Allele Consequence IMPACT SYMBOL Gene
1 C downstream_gene_variant MODIFIER KLHL17 ENSG00000187961
2 A downstream_gene_variant MODIFIER KLHL17 ENSG00000187961
3 C downstream_gene_variant MODIFIER C1orf170 ENSG00000187642
4 A downstream_gene_variant MODIFIER C1orf170 ENSG00000187642
5 C intron_variant MODIFIER PLEKHN1 ENSG00000187583
6 A intron_variant MODIFIER PLEKHN1 ENSG00000187583
7 C intron_variant MODIFIER PLEKHN1 ENSG00000187583
8 A intron_variant MODIFIER PLEKHN1 ENSG00000187583
9 C intron_variant MODIFIER PLEKHN1 ENSG00000187583
10 A intron_variant MODIFIER PLEKHN1 ENSG00000187583
11 C downstream_gene_variant MODIFIER C1orf170 ENSG00000187642
12 A downstream_gene_variant MODIFIER C1orf170 ENSG00000187642
13 C downstream_gene_variant MODIFIER C1orf170 ENSG00000187642
14 A downstream_gene_variant MODIFIER C1orf170 ENSG00000187642
15 C upstream_gene_variant MODIFIER PLEKHN1 ENSG00000187583
16 A upstream_gene_variant MODIFIER PLEKHN1 ENSG00000187583
17 C upstream_gene_variant MODIFIER PLEKHN1 ENSG00000187583
18 A upstream_gene_variant MODIFIER PLEKHN1 ENSG00000187583
Feature_type Feature BIOTYPE EXON INTRON
1 Transcript ENST00000338591 protein_coding
2 Transcript ENST00000338591 protein_coding
3 Transcript ENST00000341290 protein_coding
4 Transcript ENST00000341290 protein_coding
5 Transcript ENST00000379407 protein_coding 2/14
6 Transcript ENST00000379407 protein_coding 2/14
7 Transcript ENST00000379409 protein_coding 2/14
8 Transcript ENST00000379409 protein_coding 2/14
9 Transcript ENST00000379410 protein_coding 2/15
10 Transcript ENST00000379410 protein_coding 2/15
11 Transcript ENST00000433179 protein_coding
12 Transcript ENST00000433179 protein_coding
13 Transcript ENST00000479361 retained_intron
14 Transcript ENST00000479361 retained_intron
15 Transcript ENST00000480267 retained_intron
16 Transcript ENST00000480267 retained_intron
17 Transcript ENST00000491024 protein_coding
18 Transcript ENST00000491024 protein_coding
HGVSc HGVSp cDNA_position CDS_position
1
2
3
4
5 ENST00000379407.3:c.184-51G>C
6 ENST00000379407.3:c.184-51G>A
7 ENST00000379409.2:c.184-51G>C
8 ENST00000379409.2:c.184-51G>A
9 ENST00000379410.3:c.184-51G>C
10 ENST00000379410.3:c.184-51G>A
11
12
13
14
15
16
17
18
Protein_position Amino_acids Codons Existing_variation ALLELE_NUM DISTANCE
1 rs540662886 1 4511
2 rs540662886 2 4511
3 rs540662886 1 4978
4 rs540662886 2 4978
5 rs540662886 1
6 rs540662886 2
7 rs540662886 1
8 rs540662886 2
9 rs540662886 1
10 rs540662886 2
11 rs540662886 1 4973
12 rs540662886 2 4973
13 rs540662886 1 4979
14 rs540662886 2 4979
15 rs540662886 1 649
16 rs540662886 2 649
17 rs540662886 1 3286
18 rs540662886 2 3286
STRAND FLAGS VARIANT_CLASS MINIMISED SYMBOL_SOURCE HGNC_ID CANONICAL
1 1 SNV HGNC 24023 YES
2 1 SNV HGNC 24023 YES
3 -1 SNV HGNC 28208
4 -1 SNV HGNC 28208
5 1 SNV HGNC 25284
6 1 SNV HGNC 25284
7 1 SNV HGNC 25284
8 1 SNV HGNC 25284
9 1 SNV HGNC 25284 YES
10 1 SNV HGNC 25284 YES
11 -1 SNV HGNC 28208 YES
12 -1 SNV HGNC 28208 YES
13 -1 SNV HGNC 28208
14 -1 SNV HGNC 28208
15 1 SNV HGNC 25284
16 1 SNV HGNC 25284
17 1 cds_start_NF SNV HGNC 25284
18 1 cds_start_NF SNV HGNC 25284
TSL APPRIS CCDS ENSP SWISSPROT TREMBL UNIPARC
1 CCDS30550.1 ENSP00000343930 Q6TDP4 Q0VGE6&B3KXL7 UPI00001DFBF0
2 CCDS30550.1 ENSP00000343930 Q6TDP4 Q0VGE6&B3KXL7 UPI00001DFBF0
3 ENSP00000343864 UPI000022DAF4
4 ENSP00000343864 UPI000022DAF4
5 CCDS53256.1 ENSP00000368717 Q494U1 J3KSM5 UPI00005764FF
6 CCDS53256.1 ENSP00000368717 Q494U1 J3KSM5 UPI00005764FF
7 ENSP00000368719 Q494U1 J3KSM5 UPI0000D61E06
8 ENSP00000368719 Q494U1 J3KSM5 UPI0000D61E06
9 CCDS4.1 ENSP00000368720 Q494U1 J3KSM5 UPI00001416D8
10 CCDS4.1 ENSP00000368720 Q494U1 J3KSM5 UPI00001416D8
11 ENSP00000414022 Q5SV97 UPI0000418FB0
12 ENSP00000414022 Q5SV97 UPI0000418FB0
13
14
15
16
17 ENSP00000462558 J3KSM5 UPI000268AE1F
18 ENSP00000462558 J3KSM5 UPI000268AE1F
GENE_PHENO SIFT PolyPhen DOMAINS HGVS_OFFSET GMAF AFR_MAF AMR_MAF
1 C:0.0008 C:0 C:0
2 C:0.0008 C:0 C:0
3 C:0.0008 C:0 C:0
4 C:0.0008 C:0 C:0
5 C:0.0008 C:0 C:0
6 C:0.0008 C:0 C:0
7 C:0.0008 C:0 C:0
8 C:0.0008 C:0 C:0
9 C:0.0008 C:0 C:0
10 C:0.0008 C:0 C:0
11 C:0.0008 C:0 C:0
12 C:0.0008 C:0 C:0
13 C:0.0008 C:0 C:0
14 C:0.0008 C:0 C:0
15 C:0.0008 C:0 C:0
16 C:0.0008 C:0 C:0
17 C:0.0008 C:0 C:0
18 C:0.0008 C:0 C:0
EAS_MAF EUR_MAF SAS_MAF AA_MAF EA_MAF ExAC_MAF ExAC_Adj_MAF ExAC_AFR_MAF
1 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
2 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
3 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
4 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
5 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
6 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
7 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
8 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
9 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
10 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
11 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
12 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
13 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
14 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
15 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
16 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
17 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
18 C:0 C:0.004 C:0 C:0 C:0 C:2.146e-04
ExAC_AMR_MAF ExAC_EAS_MAF ExAC_FIN_MAF ExAC_NFE_MAF ExAC_OTH_MAF
1 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
2 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
3 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
4 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
5 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
6 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
7 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
8 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
9 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
10 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
11 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
12 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
13 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
14 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
15 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
16 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
17 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
18 C:0 C:0.0002281 C:0.002986 C:0 C:1.606e-05
ExAC_SAS_MAF CLIN_SIG SOMATIC PHENO PUBMED MOTIF_NAME MOTIF_POS HIGH_INF_POS
1 C:0
2 C:0
3 C:0
4 C:0
5 C:0
6 C:0
7 C:0
8 C:0
9 C:0
10 C:0
11 C:0
12 C:0
13 C:0
14 C:0
15 C:0
16 C:0
17 C:0
18 C:0
MOTIF_SCORE_CHANGE LoF LoF_filter LoF_flags LoF_info"
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
Code:
# load rbcf
library(rbcf)
# A vcf
filename <- "./data/rotavirus_rf.ann.vcf.gz"
# we don't need the index for this file
fp <- bcf.open(filename,FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# current variant
vc <- NULL
while(!is.null(vc<-bcf.next(fp))) {
#find the first variant having an INFO/ANN attribute
if(variant.has.attribute(vc,"ANN")) break;
}
if(!is.null(vc)) {
# get SNPEFF table
predictions<-variant.snpeff(vc)
}
# dispose the vcf reader
bcf.close(fp)
# show
predictions
Output:
[1] TRUE
Allele Annotation Annotation_Impact Gene_Name Gene_ID
1 C missense_variant MODERATE Gene_18_3284 Gene_18_3284
Feature_Type Feature_ID Transcript_BioType Rank HGVS.c HGVS.p
1 transcript AAA47319.1 protein_coding 1/1 c.952A>C p.Lys318Gln
cDNA.pos / cDNA.length CDS.pos / CDS.length AA.pos / AA.length Distance
1 952/3267 952/3267 318/1088
ERRORS / WARNINGS / INFO'"
1
Code:
# load rbcf
library(rbcf)
# create a function counting variants in a VCF, in some intervals
count.variants<-function(filename,intervals) {
# open the indexed VCF
fp <- bcf.open(filename)
# error on opening
if(is.null(fp)) return(-1)
# loop over the intervals
for(interval in intervals) {
# try query the interval
if(bcf.query(fp,interval)) {
# number of variants
n<-0
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
# increment the count
n<-n+1
}
print(paste("Number of variants in ",basename(filename),"/'",interval,"' :",n,sep=""))
}
# query failed
else {
print(paste("Cannot query ",basename(filename),"/'",interval,"'",sep=""))
}
}
# dispose the vcf reader
bcf.close(fp)
}
some_intervals <-c("","RF03","RF03:2000-3000","1:1-10000000","chr1")
count.variants("./data/rotavirus_rf.02.vcf.gz",some_intervals)
count.variants("./data/1000G.ALL.2of4intersection.20100804.genotypes.bcf",some_intervals)
# another way to query is set collect=TRUE to return a vector of variant
fp <- bcf.open("./data/rotavirus_rf.02.vcf.gz")
if(!is.null(fp)) {
print(paste("Number of variants using collect:",length(bcf.query(fp,"RF03",collect=TRUE))))
bcf.close(fp)
}
Output:
[1] "Cannot query rotavirus_rf.02.vcf.gz/''"
[1] "Number of variants in rotavirus_rf.02.vcf.gz/'RF03' :8"
[1] "Number of variants in rotavirus_rf.02.vcf.gz/'RF03:2000-3000' :4"
[1] "Cannot query rotavirus_rf.02.vcf.gz/'1:1-10000000'"
[1] "Cannot query rotavirus_rf.02.vcf.gz/'chr1'"
[1] TRUE
[1] "Cannot query 1000G.ALL.2of4intersection.20100804.genotypes.bcf/''"
[1] "Cannot query 1000G.ALL.2of4intersection.20100804.genotypes.bcf/'RF03'"
[1] "Cannot query 1000G.ALL.2of4intersection.20100804.genotypes.bcf/'RF03:2000-3000'"
[1] "Number of variants in 1000G.ALL.2of4intersection.20100804.genotypes.bcf/'1:1-10000000' :11"
[1] "Cannot query 1000G.ALL.2of4intersection.20100804.genotypes.bcf/'chr1'"
[1] TRUE
[1] "Number of variants using collect: 8"
[1] TRUE
Code:
# load rbcf
library(rbcf)
# find given variant
find.variant<-function(fp,contig,pos) {
if(!bcf.query(fp,paste(contig,":",pos,"-",pos,sep=""))) return(NULL)
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
return(vc)
}
return(NULL)
}
filename<-"./data/gnomad.exomes.r2.0.1.sites.bcf"
# open the VCF with index
fp <- bcf.open(filename)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
ctx <-find.variant(fp,"1",905608)
stopifnot(variant.has.attribute(ctx,"CSQ"))
print(paste("CSQ(no split) ",variant.string.attribute(ctx,"CSQ",split=FALSE)))
print(paste("CSQ(split) ",variant.string.attribute(ctx,"CSQ")))
stopifnot(variant.has.attribute(ctx,"AN_POPMAX"))
print(paste("AN_POPMAX:",variant.int.attribute(ctx,"AN_POPMAX")))
stopifnot(variant.has.attribute(ctx,"AF_POPMAX"))
print(paste("AF_POPMAX:",variant.float.attribute(ctx,"AF_POPMAX")))
print(paste("flag:VQSR_NEGATIVE_TRAIN_SITE:",variant.flag.attribute(ctx,"VQSR_NEGATIVE_TRAIN_SITE")))
# dispose the vcf reader
bcf.close(fp)
Output:
[1] "CSQ(no split) T|downstream_gene_variant|MODIFIER|KLHL17|ENSG00000187961|Transcript|ENST00(...)
[1] "CSQ(split) T|downstream_gene_variant|MODIFIER|KLHL17|ENSG00000187961|Transcript|ENST0000(...)
[2] "CSQ(split) A|downstream_gene_variant|MODIFIER|KLHL17|ENSG00000187961|Transcript|ENST0000(...)
[3] "CSQ(split) T|downstream_gene_variant|MODIFIER|C1orf170|ENSG00000187642|Transcript|ENST00(...)
[4] "CSQ(split) A|downstream_gene_variant|MODIFIER|C1orf170|ENSG00000187642|Transcript|ENST00(...)
[5] "CSQ(split) T|intron_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000379407|(...)
[6] "CSQ(split) A|intron_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000379407|(...)
[7] "CSQ(split) T|intron_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000379409|(...)
[8] "CSQ(split) A|intron_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000379409|(...)
[9] "CSQ(split) T|intron_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000379410|(...)
[10] "CSQ(split) A|intron_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000379410|(...)
[11] "CSQ(split) T|downstream_gene_variant|MODIFIER|C1orf170|ENSG00000187642|Transcript|ENST00(...)
[12] "CSQ(split) A|downstream_gene_variant|MODIFIER|C1orf170|ENSG00000187642|Transcript|ENST00(...)
[13] "CSQ(split) T|downstream_gene_variant|MODIFIER|C1orf170|ENSG00000187642|Transcript|ENST00(...)
[14] "CSQ(split) A|downstream_gene_variant|MODIFIER|C1orf170|ENSG00000187642|Transcript|ENST00(...)
[15] "CSQ(split) T|upstream_gene_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000(...)
[16] "CSQ(split) A|upstream_gene_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000(...)
[17] "CSQ(split) T|upstream_gene_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000(...)
[18] "CSQ(split) A|upstream_gene_variant|MODIFIER|PLEKHN1|ENSG00000187583|Transcript|ENST00000(...)
[1] "AN_POPMAX: 106408" "AN_POPMAX: 106408"
[1] "AF_POPMAX: 1.87955993169453e-05" "AF_POPMAX: 9.39778965403093e-06"
[1] "flag:VQSR_NEGATIVE_TRAIN_SITE: FALSE"
[1] TRUE
Code:
# load rbcf
library(rbcf)
# find given variant
find.variant<-function(fp,contig,pos) {
if(!bcf.query(fp,paste(contig,":",pos,"-",pos,sep=""))) return(NULL)
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
return(vc)
}
return(NULL)
}
filename<-"./data/1000G.ALL.2of4intersection.20100804.genotypes.bcf"
# open the VCF with index
fp <- bcf.open(filename)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# find a variant
ctx <-find.variant(fp,"1",10583)
print(paste("Number of genotypes ",variant.nsamples(ctx)))
# get 10-th genotype
gt<-variant.genotype(ctx,10)
print(paste("sample ",genotype.sample(gt)))
# get genotype by name
gt<-variant.genotype(ctx,"NA18997")
print(paste("sample ",genotype.sample(gt)))
print(paste("alleles ",genotype.alleles.idx0(gt)))
print(paste("genotype ploidy ? ",genotype.ploidy(gt)))
print(paste("genotype is hom ref ? ",genotype.homref(gt)))
print(paste("genotype is het ? ",genotype.het(gt)))
print(paste("genotype is het-non-ref ? ",genotype.hetnonref(gt)))
print(paste("genotype is phased ? ",genotype.phased(gt)))
print(paste("genotype is no call ? ",genotype.nocall(gt)))
print(paste("genotype FORMAT/OG ? ",genotype.string.attribute(gt,"OG")))
print(paste("genotype FORMAT/GQ ? ",genotype.int.attribute(gt,"GQ")))# hum spec says gt should be integer
print(paste("genotype has GQ ? ",genotype.has.gq(gt)))
print(paste("genotype GQ ",genotype.gq(gt)))
print(paste("genotype has DP ? ",genotype.has.dp(gt)))
print(paste("genotype DP ",genotype.int.attribute(gt,"DP")))
print(paste("genotype DP ",genotype.dp(gt)))
print(paste("genotype has PL ? ",genotype.has.pl(gt)))
print(paste("genotype PL ",genotype.pl(gt)))
print(paste("genotype has AD ? ",genotype.has.ad(gt)))
print(paste("genotype AD ",genotype.ad(gt)))
# dispose the vcf reader
bcf.close(fp)
Output:
[1] "Number of genotypes 629"
[1] "sample HG00120"
[1] "sample NA18997"
[1] "alleles 0" "alleles 1"
[1] "genotype ploidy ? 2"
[1] "genotype is hom ref ? FALSE"
[1] "genotype is het ? TRUE"
[1] "genotype is het-non-ref ? FALSE"
[1] "genotype is phased ? TRUE"
[1] "genotype is no call ? FALSE"
[1] "genotype FORMAT/OG ? 1/1"
[1] "genotype FORMAT/GQ ? "
[1] "genotype has GQ ? FALSE"
[1] "genotype GQ -1"
[1] "genotype has DP ? TRUE"
[1] "genotype DP 1"
[1] "genotype DP 1"
[1] "genotype has PL ? FALSE"
[1] "genotype PL "
[1] "genotype has AD ? TRUE"
[1] "genotype AD 4" "genotype AD 1"
[1] TRUE
Code:
# load rbcf
library(rbcf)
# find given variant
find.variant<-function(fp,contig,pos) {
if(!bcf.query(fp,paste(contig,":",pos,"-",pos,sep=""))) return(NULL)
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
return(vc)
}
return(NULL)
}
filename<-"./data/1000G.ALL.2of4intersection.20100804.genotypes.bcf"
# open the VCF with index
fp <- bcf.open(filename)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# find a variant
ctx <-find.variant(fp,"1",10583)
print(paste("Number of genotypes ",variant.nsamples(ctx)))
# Retrieve the DP for all genotypes (length of vector equals to number of samples)
dp <- variant.genotypes.int.attribute(ctx, "DP")
stopifnot(length(dp) == variant.nsamples(ctx))
stopifnot(is.integer(dp))
cat("DP: ", paste(dp, collapse = ", "), "\n")
# Retrieve the GQ for all genotypes (length of vector equals to number of samples)
gq <- variant.genotypes.float.attribute(ctx, "GQ")
stopifnot(length(gq) == variant.nsamples(ctx))
stopifnot(is.numeric(gq))
cat("GQ: ", paste(gq, collapse = ", "), "\n")
# Retrieve the AD for all genotypes (length of vector equals to 2x number of samples)
# The first two number contain the AD for REF and ALT for the first sample respectively.
ad <- variant.genotypes.int.attribute(ctx, "AD")
stopifnot(length(ad) == 2*variant.nsamples(ctx))
stopifnot(is.integer(ad))
cat("AD: ", paste(ad, collapse = ", "), "\n")
# Helper function to get all the GT indizes
gt <- variant.genotypes.allele.idx0(ctx)
stopifnot(length(gt) == 2*variant.nsamples(ctx))
stopifnot(is.integer(gt))
cat("GT - Integers: ", paste(gt, collapse = ", "), "\n")
# Helper function to get all the GT allele-counts
gt <- variant.genotypes.allele.counts(ctx, 0)
stopifnot(length(gt) == variant.nsamples(ctx))
stopifnot(is.integer(gt))
cat("GT - Allele Counts (Reference): ", paste(gt, collapse = ", "), "\n")
gtc <- variant.genotypes.allele.counts(ctx, 1)
stopifnot(length(gtc) == variant.nsamples(ctx))
stopifnot(is.integer(gtc))
cat("GT - Allele Counts (Alternative): ", paste(gtc, collapse = ", "), "\n")
# Helper function to get all the GT strings
gt <- variant.genotypes.allele.strings(ctx)
stopifnot(length(gt) == variant.nsamples(ctx))
stopifnot(is.character(gt))
cat("GT - Strings: ", paste(gt, collapse = ", "), "\n")
# dispose the vcf reader
bcf.close(fp)
Output:
[1] "Number of genotypes 629"
DP: NA, NA, NA, NA, NA, NA, 2, NA, 1, 5, NA, NA, 1, NA, NA, 3, NA, NA, NA, 4, 1, 1, 1, 1, NA, (...)
GQ: 3.27999997138977, 3.27999997138977, 3.27999997138977, 3.27999997138977, 3.27999997138977, (...)
AD: NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, NA, 2, 0, NA, NA, 5, 1, 4, 1, NA, NA, NA, NA, (...)
GT - Integers: 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0(...)
GT - Allele Counts (Reference): 2, 2, 2, 2, 2, 2, 2, 2, 2, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 1,(...)
GT - Allele Counts (Alternative): 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, (...)
GT - Strings: 0|0, 0|0, 0|0, 0|0, 0|0, 0|0, 0|0, 0|0, 0|0, 0|1, 0|0, 0|0, 0|0, 0|0, 0|0, 0|0, (...)
[1] TRUE
Code:
# load rbcf
library(rbcf)
# vcf input filename
filenamein = "./data/rotavirus_rf.01.vcf"
# output vcf filename. "-" is standard output
filenameout = "-"
fp <- bcf.open(filenamein,FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# create a new VCF writer using the header from 'fp'
out <- bcf.new.writer(fp,filenameout)
# error on opening (exit 0 for tests)
if(is.null(out)) quit(save="no",status=0,runLast=FALSE)
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
# only write POS%10==0
if(variant.pos(vc)%%10==0) {
# write variant
bcf.write.variant(out,vc);
}
}
# dispose the vcf reader
bcf.close(fp)
# dispose the vcf rwriter
bcf.close(out);
Output:
[1] TRUE
##fileformat=VCFv4.2
##FILTER=<ID=PASS,Description="All filters passed">
##samtoolsVersion=1.3.1+htslib-1.3.1
##samtoolsCommand=samtools mpileup -Ou -f rotavirus_rf.fa S1.bam S2.bam S3.bam S4.bam S5.bam
##reference=file://rotavirus_rf.fa
##contig=<ID=RF01,length=3302>
##contig=<ID=RF02,length=2687>
##contig=<ID=RF03,length=2592>
##contig=<ID=RF04,length=2362>
##contig=<ID=RF05,length=1579>
##contig=<ID=RF06,length=1356>
##contig=<ID=RF07,length=1074>
##contig=<ID=RF08,length=1059>
##contig=<ID=RF09,length=1062>
##contig=<ID=RF10,length=751>
##contig=<ID=RF11,length=666>
##ALT=<ID=*,Description="Represents allele(s) other than observed.">
##INFO=<ID=INDEL,Number=0,Type=Flag,Description="Indicates that the variant is an INDEL.">
##INFO=<ID=IDV,Number=1,Type=Integer,Description="Maximum number of reads supporting an indel">
##INFO=<ID=IMF,Number=1,Type=Float,Description="Maximum fraction of reads supporting an indel">
##INFO=<ID=DP,Number=1,Type=Integer,Description="Raw read depth">
##INFO=<ID=VDB,Number=1,Type=Float,Description="Variant Distance Bias for filtering splice-site(...)
##INFO=<ID=RPB,Number=1,Type=Float,Description="Mann-Whitney U test of Read Position Bias (bigg(...)
##INFO=<ID=MQB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality Bias (bi(...)
##INFO=<ID=BQB,Number=1,Type=Float,Description="Mann-Whitney U test of Base Quality Bias (bigge(...)
##INFO=<ID=MQSB,Number=1,Type=Float,Description="Mann-Whitney U test of Mapping Quality vs Stra(...)
##INFO=<ID=SGB,Number=1,Type=Float,Description="Segregation based metric.">
##INFO=<ID=MQ0F,Number=1,Type=Float,Description="Fraction of MQ0 reads (smaller is better)">
##FORMAT=<ID=PL,Number=G,Type=Integer,Description="List of Phred-scaled genotype likelihoods">
##FORMAT=<ID=GT,Number=1,Type=String,Description="Genotype">
##INFO=<ID=ICB,Number=1,Type=Float,Description="Inbreeding Coefficient Binomial test (bigger is(...)
##INFO=<ID=HOB,Number=1,Type=Float,Description="Bias in the number of HOMs number (smaller is b(...)
##INFO=<ID=AC,Number=A,Type=Integer,Description="Allele count in genotypes for each ALT allele,(...)
##INFO=<ID=AN,Number=1,Type=Integer,Description="Total number of alleles in called genotypes">
##INFO=<ID=DP4,Number=4,Type=Integer,Description="Number of high-quality ref-forward , ref-reve(...)
##INFO=<ID=MQ,Number=1,Type=Integer,Description="Average mapping quality">
##bcftools_callVersion=1.3-10-g820e1d6+htslib-1.2.1-267-g87141ea
##bcftools_callCommand=call -vm -Oz -o rotavirus_rf.vcf.gz -
##bcftools_viewVersion=1.10-6-g2782d9f+htslib-1.2.1-1336-g7c16b56-dirty
##bcftools_viewCommand=view /home/lindenb/src/jvarkit/src/test/resources/rotavirus_rf.vcf.gz; D(...)
#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT S1 S2 S3 S4 S5
RF01 970 . A C 48.6696 . DP=36;VDB=0.693968;SGB=10.3229;RPB=0.658863;MQB=1;MQSB=1;BQB=0.572843;(...)
RF03 2150 . T A 6.90687 . DP=37;VDB=0.557348;SGB=-1.00336;RPB=0.579851;MQB=1;MQSB=1;BQB=1;MQ0F=(...)
RF04 1900 . A C 36.8224 . DP=39;VDB=0.706942;SGB=7.10992;RPB=0.81363;MQB=1;MQSB=1;BQB=1;MQ0F=0;(...)
RF04 1920 . A T 42.014 . DP=39;VDB=0.966939;SGB=0.816387;RPB=0.864752;MQB=1;MQSB=1;BQB=0.864752(...)
Code:
# load rbcf
library(rbcf)
# vcf input filename
filenamein = "./data/1000G.ALL.2of4intersection.20100804.genotypes.bcf"
# output vcf filename. "-" is standard output
filenameout = "-"
fp <- bcf.open(filenamein,FALSE)
# error on opening (exit 0 for tests)
if(is.null(fp)) quit(save="no",status=0,runLast=FALSE)
# create a new VCF writer using the header from 'fp'
out <- bcf.new.writer(fp,filenameout)
# error on opening (exit 0 for tests)
if(is.null(out)) quit(save="no",status=0,runLast=FALSE)
n_samples <- bcf.nsamples(fp)
# loop while we can read a variant
while(!is.null(vc<-bcf.next(fp))) {
# Setting of INT attributes
old_dp <- variant.genotypes.int.attribute(vc, "DP")
new_dp <- rnbinom(n = n_samples, size = 5, prob = 0.25)
variant.genotypes.set.int.attribute(vc, "DP", new_dp)
stopifnot( all( new_dp == variant.genotypes.int.attribute(vc, "DP") ))
old_gq <- variant.genotypes.float.attribute(vc, "GQ")
new_gq <- rnorm(n = n_samples)
variant.genotypes.set.float.attribute(vc, "GQ", new_gq)
#stopifnot( all( new_gq == variant.genotypes.float.attribute(vc, "GQ") ))
# Updating GT with random allele combinations
alleles <- c(".", as.character(0:variant.nalleles(vc)))
all_potential_genotypes = c(".",
apply(as.matrix(expand.grid(alleles, alleles)), 1, paste, collapse = "/"),
apply(as.matrix(expand.grid(0:variant.nalleles(vc), 0:variant.nalleles(vc))), 1, paste, collapse = "|")
)
new_gt <- sample(x = all_potential_genotypes, size = n_samples, replace = TRUE)
variant.genotypes.set.allele.strings(vc, new_gt)
new_gt <- sub("-", ".", new_gt)
subset(data.frame( Expected = new_gt, Seen = variant.genotypes.allele.strings(vc)), Expected != Seen)
stopifnot(all( new_gt == variant.genotypes.allele.strings(vc) ))
bcf.write.variant(out, vc)
}
# dispose the vcf reader
bcf.close(fp)
# dispose the vcf rwriter
bcf.close(out)
Output:
##fileformat=VCFv4.0
##FILTER=<ID=PASS,Description="All filters passed">
##filedat=20101112
##datarelease=20100804
##samples=629
##contig=<ID=1,length=249250621>
##description="Where BI calls are present, genotypes and alleles are from BI. In there absence(...)
##FORMAT=<ID=AD,Number=A,Type=Integer,Description="Allelic depths for the ref and alt alleles i(...)
##FORMAT=<ID=DP,Number=1,Type=Integer,Description="Read Depth (only filtered reads used for cal(...)
##FORMAT=<ID=GL,Number=G,Type=Float,Description="Log-scaled likelihoods for AA,AB,BB genotypes (...)
##FORMAT=<ID=GQ,Number=1,Type=Float,Description="Genotype Quality">
##FORMAT=<ID=GT,Number=A,Type=String,Description="Genotype">
##FORMAT=<ID=GD,Number=1,Type=Float,Description="Genotype dosage. Expected count of non-ref al(...)
##FORMAT=<ID=OG,Number=1,Type=String,Description="Original Genotype input to Beagle">
##INFO=<ID=AF,Number=.,Type=Float,Description="Allele Frequency, for each ALT allele, in the sa(...)
##INFO=<ID=DP,Number=1,Type=Integer,Description="Total Depth">
##INFO=<ID=CB,Number=.,Type=String,Description="List of centres that called, UM (University of (...)
##INFO=<ID=EUR_R2,Number=1,Type=Float,Description="R2 From Beagle based on European Samples">
##INFO=<ID=AFR_R2,Number=1,Type=Float,Description="R2 From Beagle based on AFRICAN Samples">
##INFO=<ID=ASN_R2,Number=1,Type=Float,Description="R2 From Beagle based on Asian Samples">
##bcftools_viewVersion=1.9-321-g5774f32+htslib-1.10.2-22-gbfc9f0d
##bcftools_viewCommand=view -O b -o 1000G.ALL.2of4intersection.20100804.genotypes.bcf 1000G.ALL(...)
#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT HG00098 HG00100 HG00106 HG00112 HG00114 HG00116 H(...)
1 10583 rs58108140 G A . 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[1] TRUE