ui bug fix

software/control/core/core.py

@@ -4931,10 +4931,10 @@ def set_reference(self) -> bool:
 
         # Store cropped and normalized reference image
         center_y = int(reference_image.shape[0] / 2)
-        x_start = max(0, int(x) - self.SPOT_CROP_SIZE // 2)
-        x_end = min(reference_image.shape[1], int(x) + self.SPOT_CROP_SIZE // 2)
-        y_start = max(0, center_y - self.SPOT_CROP_SIZE // 2)
-        y_end = min(reference_image.shape[0], center_y + self.SPOT_CROP_SIZE // 2)
+        x_start = max(0, int(x) - self.laser_af_properties.spot_crop_size // 2)
+        x_end = min(reference_image.shape[1], int(x) + self.laser_af_properties.spot_crop_size // 2)
+        y_start = max(0, center_y - self.laser_af_properties.spot_crop_size // 2)
+        y_end = min(reference_image.shape[0], center_y + self.laser_af_properties.spot_crop_size // 2)
 
         reference_crop = reference_image[y_start:y_end, x_start:x_end].astype(np.float32)
         self.reference_crop = (reference_crop - np.mean(reference_crop)) / np.max(reference_crop)

software/control/gui_hcs.py

@@ -996,6 +996,9 @@ def connect_objective_changed_laser_af():
             self.laserAutofocusSettingWidget.signal_newAnalogGain.connect(
                 self.cameraSettingWidget_focus_camera.set_analog_gain
             )
+            self.laserAutofocusSettingWidget.signal_apply_settings.connect(
+                self.laserAutofocusControlWidget.update_init_state
+            )
 
             self.laserAutofocusSettingWidget.update_exposure_time(self.laserAutofocusSettingWidget.exposure_spinbox.value())
             self.laserAutofocusSettingWidget.update_analog_gain(self.laserAutofocusSettingWidget.analog_gain_spinbox.value())

software/control/widgets.py

@@ -348,7 +348,7 @@ class LaserAutofocusSettingWidget(QWidget):
 
     signal_newExposureTime = Signal(float)
     signal_newAnalogGain = Signal(float)
-    signal_start_live = Signal()
+    signal_apply_settings = Signal()
 
     def __init__(self, liveController, laserAutofocusController, stretch=True):
         super().__init__()
@@ -387,7 +387,6 @@ def init_ui(self):
         self.exposure_spinbox = QDoubleSpinBox()
         self.exposure_spinbox.setRange(self.liveController.camera.EXPOSURE_TIME_MS_MIN, self.liveController.camera.EXPOSURE_TIME_MS_MAX)
         self.exposure_spinbox.setValue(self.laserAutofocusController.laser_af_properties.focus_camera_exposure_time_ms)
-        self.exposure_spinbox.setDecimals(1)
         exposure_layout.addWidget(self.exposure_spinbox)
 
         # Analog gain control
@@ -514,6 +513,9 @@ def _add_spinbox(self, layout, label: str, property_name: str,
         self.spinboxes[property_name] = spinbox
 
     def toggle_live(self, pressed):
+        # Print traceback for debugging
+        import traceback
+        traceback.print_stack()
         if pressed:
             self.liveController.start_live()
             self.btn_live.setText("Stop Live")
@@ -568,6 +570,7 @@ def apply_settings(self):
         }
         self.laserAutofocusController.set_laser_af_properties(updates)
         self.laserAutofocusController.initialize_auto()
+        self.signal_apply_settings.emit()
         self.update_calibration_label()
 
     def update_calibration_label(self):