FAS is a new release of the original FACT algorithm. It calculates the so called FAS-score which is a measure of how similar the feature architectures of two proteins are. This is done by combining the Multiplicity Score (MS) and the Positional Score (PS) from FACT. Unlike the original FACT, FAS can resolve feature architectures that have overlapping features by searching for the best overlap-free path. This can be done either extensively or by using the priority mode, a greedy approach. FAS also allows for more options in the weighting of features.
FAS is provided as a python package and compatible with Python3.
You can install FAS with pip:
python3 -m pip install greedyFAS
(*) In case you do not have admin rights, and don't use package systems like Anaconda to manage environments you need to use the --user option (not recommended):
python3 -m pip install --user greedyFAS
and then add the following line to the end of your .bashrc or .bash_profile file, restart the current terminal to apply the change:
export PATH=$HOME/.local/bin:$PATH
Before using FAS, some annotation tools and databases need to be installed. FAS' standard databases/annotation tools are: PFAM, SMART, fLPS, SEG, COILS, THMHH 2.0c and SignalP 4.1g. To get these tools and make a configuration file for FAS, please use the setupFAS function:
fas.setup -t /directory/where/you/want/to/save/annotation/tools
Inside the output directory you will find a file called annoTools.txt that contains all installed annotation tools. If you wish to discard any of them from the annotation process, you can just remove the unneeded tools from that file.
Please read our wiki page of setupFAS for other use-cases, such as how to use your old annotation tools with the new FAS, etc.
NOTE: we provide compiled code only for PFAM, COILS and SEG. fLPS will be automatically downloaded and installed. For SMART, you need to download it from EMBLEM and give the path to fas.setup. For TMHMM and SignalP, you can decide if you want to include those two tools to the annotation step (recommended) or ignore them. For using TMHMM version 2.0c and SignalP version 4.1g, you need to request a license from the authors at https://services.healthtech.dtu.dk, and save the downloaded files in the same directory. FAS will do the rest for you ;-)
NOTE2: SignalP 5.0b is not supported yet!!!
We suggest you test the annotation tools by running this command:
fas.doAnno -i test_annofas.fa -o test_output
test_annofas.fa is a demo multiple fasta file, which is saved in the installed greedyFAS directory.
If you only want to annotate your protein sequences without calculating the FAS scores, you can use the doAnno function.
fas.doAnno --fasta your_proteins.fa --outPath /annotation/path/
The annotation output (your_proteins.json by default) will be saved in /annotation/path/.
Alternatively, you can do the annotation using InterProScan and use the function parseAnno to convert the InterProScan's tsv output into json format for using with FAS.
fas.parseAnno -i INPUT.tsv -o /annotation/path/INPUT.json -t <tool_name> -f <feature columns> ...
Please check the usage of parseAnno for more info (using fas.parseAnno -h).
The main purpose of FAS is to calculate the similarity score between 2 given proteins (or two list of proteins). This can be done using the run function.
fas.run -s seed.fa -q query.fa -a /annotation/path/ -o /output/path/
If the annotations of seed and query protein(s) already exist in /annotation/path/ (seed.json and query.json, respectively), run will use these annotations for calculating the FAS scores. Otherwise, it will first annotate the proteins and then compare the feature architectures of those two protein sets.
A thorough guide to all FAS commands and options can be found at our WIKI page.
Julian Dosch, Holger Bergmann, Vinh Tran, Ingo Ebersberger, FAS: assessing the similarity between proteins using multi-layered feature architectures, Bioinformatics, Volume 39, Issue 5, May 2023, btad226, https://doi.org/10.1093/bioinformatics/btad226
Julian Dosch [email protected]
Ingo Ebersberger [email protected]