diff --git a/samples/drivers/i2c/rtio_loopback/sample.yaml b/samples/drivers/i2c/rtio_loopback/sample.yaml
index e7d5563f5b1b..4234c090c67e 100644
--- a/samples/drivers/i2c/rtio_loopback/sample.yaml
+++ b/samples/drivers/i2c/rtio_loopback/sample.yaml
@@ -5,6 +5,9 @@ tests:
     tags:
       - rtio
       - i2c_target
+    harness: ztest
+    harness_config:
+      fixture: i2c_bus_short
     platform_allow:
       - b_u585i_iot02a
       - nrf5340dk/nrf5340/cpuapp