diff --git a/webnn/conformance_tests/not_equal.https.any.js b/webnn/conformance_tests/not_equal.https.any.js
new file mode 100644
index 000000000000000..dc02620f07c62c5
--- /dev/null
+++ b/webnn/conformance_tests/not_equal.https.any.js
@@ -0,0 +1,537 @@
+// META: title=test WebNN API element-wise notEqual operation
+// META: global=window,dedicatedworker
+// META: variant=?cpu
+// META: variant=?gpu
+// META: variant=?npu
+// META: script=../resources/utils.js
+// META: timeout=long
+
+'use strict';
+
+// https://github.com/webmachinelearning/webnn/issues/375#issuecomment-2292466613
+// Compare if the values of the two input tensors are not equal, element-wise.
+//
+// MLOperand notEqual(MLOperand a, MLOperand b);
+
+
+const getNotEqualPrecisionTolerance = (graphResources) => {
+  const toleranceValueDict = {uint8: 0};
+  const expectedDataType =
+      getExpectedDataTypeOfSingleOutput(graphResources.expectedOutputs);
+  return {metricType: 'ULP', value: toleranceValueDict[expectedDataType]};
+};
+
+const notEqualTests = [
+  {
+    'name': 'notEqual float32 0D scalar',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [-0.6285496950149536],
+          'descriptor': {shape: [], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [-4.4166412353515625],
+          'descriptor': {shape: [], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {'data': [1], 'descriptor': {shape: [], dataType: 'uint8'}}
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 1D constant tensors',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [24], dataType: 'float32'},
+          'constant': true
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,  -4.839719772338867,
+            4.996258735656738,   0.9727277755737305, -6.173707485198975,
+            2.80570650100708,    5.588105201721191,  7.767369747161865,
+            -4.308907985687256,  -5.895479679107666, -8.53209114074707,
+            2.80570650100708,    5.588105201721191,  0.17833954095840454,
+            -4.479541778564453,  0.6819732189178467, -6.6875128746032715,
+            2.80570650100708,    5.588105201721191,  -9.041799545288086,
+            -1.9728281497955322, -3.011512279510498, 3.6268343925476074
+          ],
+          'descriptor': {shape: [24], dataType: 'float32'},
+          'constant': true
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 0, 1, 0, 0, 1, 0, 0, 1, 1, 1, 1,
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [24], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 1D tensors',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [24], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,  -4.839719772338867,
+            4.996258735656738,   0.9727277755737305, -6.173707485198975,
+            2.80570650100708,    5.588105201721191,  7.767369747161865,
+            -4.308907985687256,  -5.895479679107666, -8.53209114074707,
+            2.80570650100708,    5.588105201721191,  0.17833954095840454,
+            -4.479541778564453,  0.6819732189178467, -6.6875128746032715,
+            2.80570650100708,    5.588105201721191,  -9.041799545288086,
+            -1.9728281497955322, -3.011512279510498, 3.6268343925476074
+          ],
+          'descriptor': {shape: [24], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 0, 1, 0, 0, 1, 0, 0, 1, 1, 1, 1,
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [24], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 2D tensors',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [4, 6], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,  -4.839719772338867,
+            4.996258735656738,   0.9727277755737305, -6.173707485198975,
+            2.80570650100708,    5.588105201721191,  7.767369747161865,
+            -4.308907985687256,  -5.895479679107666, -8.53209114074707,
+            2.80570650100708,    5.588105201721191,  0.17833954095840454,
+            -4.479541778564453,  0.6819732189178467, -6.6875128746032715,
+            2.80570650100708,    5.588105201721191,  -9.041799545288086,
+            -1.9728281497955322, -3.011512279510498, 3.6268343925476074
+          ],
+          'descriptor': {shape: [4, 6], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 0, 1, 0, 0, 1, 0, 0, 1, 1, 1, 1,
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [4, 6], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 3D tensors',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 3, 4], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,  -4.839719772338867,
+            4.996258735656738,   0.9727277755737305, -6.173707485198975,
+            2.80570650100708,    5.588105201721191,  7.767369747161865,
+            -4.308907985687256,  -5.895479679107666, -8.53209114074707,
+            2.80570650100708,    5.588105201721191,  0.17833954095840454,
+            -4.479541778564453,  0.6819732189178467, -6.6875128746032715,
+            2.80570650100708,    5.588105201721191,  -9.041799545288086,
+            -1.9728281497955322, -3.011512279510498, 3.6268343925476074
+          ],
+          'descriptor': {shape: [2, 3, 4], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 0, 1, 0, 0, 1, 0, 0, 1, 1, 1, 1,
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 3, 4], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 4D tensors',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,  -4.839719772338867,
+            4.996258735656738,   0.9727277755737305, -6.173707485198975,
+            2.80570650100708,    5.588105201721191,  7.767369747161865,
+            -4.308907985687256,  -5.895479679107666, -8.53209114074707,
+            2.80570650100708,    5.588105201721191,  0.17833954095840454,
+            -4.479541778564453,  0.6819732189178467, -6.6875128746032715,
+            2.80570650100708,    5.588105201721191,  -9.041799545288086,
+            -1.9728281497955322, -3.011512279510498, 3.6268343925476074
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 0, 1, 0, 0, 1, 0, 0, 1, 1, 1, 1,
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 5D tensors',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 2, 1, 2, 3], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,  -4.839719772338867,
+            4.996258735656738,   0.9727277755737305, -6.173707485198975,
+            2.80570650100708,    5.588105201721191,  7.767369747161865,
+            -4.308907985687256,  -5.895479679107666, -8.53209114074707,
+            2.80570650100708,    5.588105201721191,  0.17833954095840454,
+            -4.479541778564453,  0.6819732189178467, -6.6875128746032715,
+            2.80570650100708,    5.588105201721191,  -9.041799545288086,
+            -1.9728281497955322, -3.011512279510498, 3.6268343925476074
+          ],
+          'descriptor': {shape: [2, 2, 1, 2, 3], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 0, 1, 0, 0, 1, 0, 0, 1, 1, 1, 1,
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 2, 1, 2, 3], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 broadcast 0D to 4D',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [2.80570650100708],
+          'descriptor': {shape: [], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 1, 1, 1, 1, 1, 0, 1, 1, 1, 1, 1,
+            0, 1, 1, 1, 1, 1, 0, 1, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 broadcast 1D to 4D',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [2.80570650100708],
+          'descriptor': {shape: [1], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 1, 1, 1, 1, 1, 0, 1, 1, 1, 1, 1,
+            0, 1, 1, 1, 1, 1, 0, 1, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 broadcast 2D to 4D',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708, 5.588105201721191, -4.9622955322265625,
+            -2.863192081451416, -3.011512279510498, 3.6268343925476074
+          ],
+          'descriptor': {shape: [2, 3], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1,
+            0, 0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 broadcast 3D to 4D',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708, 5.588105201721191, -9.041799545288086,
+            3.6268343925476074
+          ],
+          'descriptor': {shape: [2, 2, 1], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,
+            0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'uint8'}
+        }
+      }
+    }
+  },
+  {
+    'name': 'notEqual float32 broadcast 4D to 4D',
+    'graph': {
+      'inputs': {
+        'inputA': {
+          'data': [2.80570650100708],
+          'descriptor': {shape: [1, 1, 1, 1], dataType: 'float32'}
+        },
+        'inputB': {
+          'data': [
+            2.80570650100708,    5.588105201721191,   2.855226516723633,
+            4.996258735656738,   0.9727277755737305,  -4.742599964141846,
+            2.80570650100708,    5.588105201721191,   -5.107602119445801,
+            6.624142169952393,   -2.3207247257232666, -7.053895950317383,
+            2.80570650100708,    5.588105201721191,   4.980423927307129,
+            -5.440841197967529,  1.1459590196609497,  7.774532794952393,
+            2.80570650100708,    5.588105201721191,   -6.245251178741455,
+            -2.8490731716156006, -2.6951117515563965, 5.817563056945801
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'float32'}
+        }
+      },
+      'operators': [{
+        'name': 'notEqual',
+        'arguments': [{'a': 'inputA'}, {'b': 'inputB'}],
+        'outputs': 'output'
+      }],
+      'expectedOutputs': {
+        'output': {
+          'data': [
+            0, 1, 1, 1, 1, 1, 0, 1, 1, 1, 1, 1,
+            0, 1, 1, 1, 1, 1, 0, 1, 1, 1, 1, 1
+          ],
+          'descriptor': {shape: [2, 2, 2, 3], dataType: 'uint8'}
+        }
+      }
+    }
+  }
+];
+
+if (navigator.ml) {
+  notEqualTests.forEach((test) => {
+    webnn_conformance_test(
+        buildAndExecuteGraph, getNotEqualPrecisionTolerance, test);
+  });
+} else {
+  test(() => assert_implements(navigator.ml, 'missing navigator.ml'));
+}
diff --git a/webnn/validation_tests/elementwise-logical.https.any.js b/webnn/validation_tests/elementwise-logical.https.any.js
index 3db25c106246cf3..3dce605fa657833 100644
--- a/webnn/validation_tests/elementwise-logical.https.any.js
+++ b/webnn/validation_tests/elementwise-logical.https.any.js
@@ -13,6 +13,7 @@ const kElementwiseLogicalBinaryOperators = [
   'greaterOrEqual',
   'lesser',
   'lesserOrEqual',
+  'notEqual',
   'logicalAnd',
   'logicalOr',
   'logicalXor',