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Setting -f to hyphy outputs a matrix comprising mostly 100 entries.
To reproduce, I retrieve NCBI PopSet 1892228972 and downloaded the FASTA file as sequence.fasta:
art@Wernstrom Downloads % mafft sequence.fasta > hiv.mafft.fa[ omit output ]art@Wernstrom Downloads % tn93 -o temp.csv hiv.mafft.fa Read 602 sequences of length 1094Will perform 180901 pairwise distance calculationsProgress: 100% ( 2210 links found, inf evals/sec){ "Actual comparisons performed" :180901, "Comparisons accounting for copy numbers " :180901, "Total comparisons possible" : 180901, "Links found" : 2210, "Maximum distance" : 0.159834, "Sequences" : 602, "Mean distance" : 0.0907582,[ truncate output ]art@Wernstrom Downloads % head -n5 temp.csv ID1,ID2,DistanceMT787751.1 HIV-1 isolate 16854 from Italy nonfunctional pol protein (pol) gene, partial sequence,MT787795.1 HIV-1 isolate 16786 from Italy pol protein (pol) gene, partial cds,0.00367472MT787751.1 HIV-1 isolate 16854 from Italy nonfunctional pol protein (pol) gene, partial sequence,MT787869.1 HIV-1 isolate 17835 from Italy pol protein (pol) gene, partial cds,0.0139596MT787751.1 HIV-1 isolate 16854 from Italy nonfunctional pol protein (pol) gene, partial sequence,MT787972.1 HIV-1 isolate 2498686 from Italy pol protein (pol) gene, partial cds,0.0130311MT787751.1 HIV-1 isolate 16854 from Italy nonfunctional pol protein (pol) gene, partial sequence,MT788268.1 HIV-1 isolate 520925 from Italy pol protein (pol) gene, partial cds,0.012028art@Wernstrom Downloads % tn93 -o temp.txt -f hyphy hiv.mafft.fa[ omit output ]art@Wernstrom Downloads % head -n3 temp.txt{
{0,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100}
{100,0,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,0.00367472,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,0.0139596,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,0.0130311,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,0.012028,100,100,0.00552784,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100,100}
Ah, it's not a bug. Distances are only being stored in the matrix if they fall below the -t threshold, which defaults to 0.015. Setting -t1 reports out all distances. Unexpected behaviour though :-)
What do you think the appropriate modification should be? One possibility is to report N/A or null in the matrix for distances that have not been computed.
Setting
-f
tohyphy
outputs a matrix comprising mostly100
entries.To reproduce, I retrieve NCBI PopSet 1892228972 and downloaded the FASTA file as
sequence.fasta
:See also #16
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