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BUG: Incorrect SRA files (RNA-seq) #962

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simonvh opened this issue Mar 17, 2023 · 1 comment
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BUG: Incorrect SRA files (RNA-seq) #962

simonvh opened this issue Mar 17, 2023 · 1 comment
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@simonvh
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simonvh commented Mar 17, 2023

Describe the bug

Some SRA files are incorrect (either due to submitter not supplying correct files, or SRA not having correct annotation). For example, this file:
SRR18972600 (GSM6077643). This marked as PE, but fastq-dump only gets one fastq file.

To Reproduce

RNA-seq workflow, with the following samples.tsv:

sample  assembly
GSM6077643     GRCh38

Expected behavior
I don't know! Either an informative error message (i.e. two fastq files should be there, there's only one, this can be checked) or the file being treated as single-end. The second option may not be correct, and I assume this may also be hard to implement. I would guess an error message would already be good, because the error is not easily identifiable from the log, but only when you manually repeat the steps.

One thing I noticed that these are not sequenced with Illumina, but with DNBSEQ-G400.

Low priority bug I would say, but something to be aware of.

@simonvh simonvh added the bug Something isn't working label Mar 17, 2023
@Maarten-vd-Sande
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Related to #228

We can't really fix this (we don't want) as it's not really our problem 😇

However what we can do is make the error message clear, which I think is actually a good idea 😄.

If DNBSEQ-G400 never works you can add it here so that seq2science reports a nice message.

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