diff --git a/packages/component-base/src/virtualizer-iron-list-adapter.js b/packages/component-base/src/virtualizer-iron-list-adapter.js
index 1ebe4c00ab..f1474c56a6 100644
--- a/packages/component-base/src/virtualizer-iron-list-adapter.js
+++ b/packages/component-base/src/virtualizer-iron-list-adapter.js
@@ -5,7 +5,7 @@
  */
 /* eslint-disable @typescript-eslint/member-ordering */
 // https://github.com/vaadin/eslint-config-vaadin/issues/33
-import { animationFrame, timeOut } from './async.js';
+import { animationFrame, microTask, timeOut } from './async.js';
 import { isSafari } from './browser-utils.js';
 import { Debouncer, flush } from './debounce.js';
 import { ironList } from './iron-list-core.js';
@@ -350,6 +350,9 @@ export class IronListAdapter {
     // Schedule and flush a resize handler
     this._resizeHandler();
     flush();
+    // Schedule an update to ensure item positions are correct after subsequent size changes
+    // Fix for https://github.com/vaadin/flow-components/issues/6269
+    this._debounce('_update', this._update, microTask);
   }
 
   /** @private */
diff --git a/packages/component-base/test/virtualizer-variable-row-height.test.js b/packages/component-base/test/virtualizer-variable-row-height.test.js
index 6d6146e41f..2fa7c1fbad 100644
--- a/packages/component-base/test/virtualizer-variable-row-height.test.js
+++ b/packages/component-base/test/virtualizer-variable-row-height.test.js
@@ -1,5 +1,5 @@
 import { expect } from '@esm-bundle/chai';
-import { fixtureSync, nextFrame } from '@vaadin/testing-helpers';
+import { aTimeout, fixtureSync, isFirefox, nextFrame } from '@vaadin/testing-helpers';
 import sinon from 'sinon';
 import { Virtualizer } from '../src/virtualizer.js';
 
@@ -204,7 +204,12 @@ describe('virtualizer - variable row height - large variance', () => {
     await fixItemPositioningTimeout();
     const targetScrollTop = scrollTarget.scrollHeight - scrollTarget.offsetHeight;
 
-    expect(scrollTarget.scrollTop).to.equal(targetScrollTop);
+    if (isFirefox) {
+      // Firfox has a rounding issue that requires a small tolerance
+      expect(scrollTarget.scrollTop).to.be.closeTo(targetScrollTop, 1);
+    } else {
+      expect(scrollTarget.scrollTop).to.equal(targetScrollTop);
+    }
   });
 
   it('should allow scrolling to start', async () => {
@@ -233,3 +238,52 @@ describe('virtualizer - variable row height - large variance', () => {
     expect(virtualizer.__adapter.__fixInvalidItemPositioning.callCount).to.equal(0);
   });
 });
+
+describe('virtualizer - variable row height - size changes', () => {
+  let virtualizer;
+
+  beforeEach(async () => {
+    const reverseItemHeights = [50, 30];
+
+    const scrollTarget = fixtureSync(`
+      <div style="height: 500px; width: 200px;">
+        <div></div>
+      </div>
+    `);
+    const scrollContainer = scrollTarget.firstElementChild;
+
+    virtualizer = new Virtualizer({
+      createElements: (count) => Array.from(Array(count)).map(() => document.createElement('div')),
+      updateElement: (el, index) => {
+        el.style.height = `${reverseItemHeights[virtualizer.size - index - 1]}px`;
+        el.style.outline = '1px solid black';
+        el.style.outlineOffset = '-1px';
+        el.style.width = '100%';
+        el.textContent = `Item ${index}`;
+        el.id = `item-${index}`;
+      },
+      scrollTarget,
+      scrollContainer,
+    });
+
+    virtualizer.size = 2;
+    // Wait for a possible resize observer flush
+    await aTimeout(100);
+  });
+
+  it('should position the items correctly after subsequent size changes', async () => {
+    virtualizer.size = 1;
+    virtualizer.update();
+
+    virtualizer.size = 2;
+    virtualizer.update();
+
+    await nextFrame();
+
+    const item0 = document.getElementById('item-0');
+    const item1 = document.getElementById('item-1');
+    expect(item0.clientHeight).to.equal(30);
+    expect(item1.clientHeight).to.equal(50);
+    expect(item1.getBoundingClientRect().top).to.equal(item0.getBoundingClientRect().bottom);
+  });
+});