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format-version: 1.2
date: 14:07:2015 14:01
saved-by: kvillanova
auto-generated-by: OBO-Edit 2.3.1
default-namespace: eco
[Term]
id: ECO:0001501
name: microbiological assay evidence
def: "A type of evidence based on microbial assays." []
creation_date: 2015-06-19T13:28:13Z
[Term]
id: ECO:0001502
name: differential medium evidence
def: "A type of microbiological medium evidence where a medium has agents that allow for the growth of a wide range of microbial organisms, but also expresses the visual differences of organisms." []
synonym: "differential media evidence" EXACT []
is_a: ID:0001514 ! microbiological medium evidence
created_by: mchibucos
creation_date: 2015-06-19T13:28:45Z
[Term]
id: ID:0001501
name: selective medium evidence
def: "A type of microbiological medium evidence where a medium is selective. The medium has one or more agents that selectively inhibits the growth of certain microbial organisms thereby allowing for the growth of other types of organisms." [ECO:KAV, PMID:7068837, PMID:8434926]
comment: A common inhibitory agent for selective media are antibiotics. Examples include ampicillins, tetracyclines, vancomycin etc.
synonym: "<new synonym>" RELATED []
synonym: "selective media evidence " EXACT []
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-19T14:42:01Z
[Term]
id: ID:0001502
name: acid-fast staining evidence
def: "A type of differential staining evidence based on any type of stain used to view acid fast microorganisms." [ECO:KAV, PMID:24619760, PMID:9885935]
comment: The acid fast stain is particularly known for its ability to stain Mycobacterium and some strains of the Nocardia species.
synonym: "acid-fast stain evidence" EXACT []
synonym: "AFS evidence" EXACT []
is_a: ID:0001505 ! differential staining evidence
created_by: kvillanova
creation_date: 2015-06-19T15:29:56Z
[Term]
id: ID:0001503
name: Place holder ( merge thease terms with) ECO:0005019 Staining evidence
def: "A type of microbiological assay evidence where dyes are used to stain slides with microbiological specimens.\n\nsuggested parent term ECO:0005019 staining evidence" [ECO:KAV]
synonym: "microbiological staining evidence" EXACT []
is_a: ECO:0001501 ! microbiological assay evidence
created_by: kvillanova
creation_date: 2015-06-19T14:43:08Z
[Term]
id: ID:0001504
name: simple staining evidence
def: "A type of staining evidence where one dye is used to stain a specimen to verify presence and display morphology." [ECP:KAV, PMID:13094012]
synonym: "simple stain evidence" EXACT []
is_a: ID:0001503 ! Place holder ( merge thease terms with) ECO:0005019 Staining evidence
created_by: kvillanova
creation_date: 2015-06-19T14:45:17Z
[Term]
id: ID:0001505
name: differential staining evidence
def: "A type of staining evidence where a primary dye and a counter-stain are. Used to differentiate between specimen morphologies." [ECO:KAV]
synonym: "differential stain evidence " EXACT []
is_a: ID:0001503 ! Place holder ( merge thease terms with) ECO:0005019 Staining evidence
created_by: kvillanova
creation_date: 2015-06-19T14:45:51Z
[Term]
id: ID:0001506
name: microbiological culture evidence
is_a: ECO:0001501 ! microbiological assay evidence
created_by: kvillanova
creation_date: 2015-06-19T14:48:31Z
[Term]
id: ID:0001507
name: camp test evidence
is_a: ID:0001509 ! microbiological biochemical evidence
created_by: kvillanova
creation_date: 2015-06-19T14:48:54Z
[Term]
id: ID:0001508
name: microscopy evidence (place holder)
def: "Correct parent when merged should be ECO:0001098" []
is_a: ID:0001597 ! placeholder
created_by: kvillanova
creation_date: 2015-06-19T16:08:45Z
[Term]
id: ID:0001509
name: microbiological biochemical evidence
is_a: ECO:0001501 ! microbiological assay evidence
created_by: kvillanova
creation_date: 2015-06-19T14:50:10Z
[Term]
id: ID:0001510
name: microbiological smear evidence
is_a: ECO:0001501 ! microbiological assay evidence
created_by: kvillanova
creation_date: 2015-06-19T16:22:32Z
[Term]
id: ID:0001511
name: polarized microscopy evidence
def: "A type of light microscopy evidence based on any type of micrscope that uses polarized light." [ECO:KAV, PMCID:PMC1035042, PMID:11159464]
is_a: ID:0001535 ! light microscopy evidence
created_by: kvillanova
creation_date: 2015-07-14T12:57:25Z
[Term]
id: ID:0001513
name: gram staining evidence
def: "A type of differential staining evidence where crystal violet is used as the primary dye and safranin is used as the counterstain. The stain is named after Hans Christain Gram who developed it in 1894 while working with Carl Friedländer in the Berlin city morge. The gram stain is used to separate bacteria into two major categories gram positive and gram negative, gram positive having thick peptidoglycan and the gram negative having thin peptidoglycan. The protocol for the stain is an application of the crystal violet followed by Gram's iodine as the mordant, ethyl alcohol as a decolorizer, and safranin as the counterstain." [ECO:KAV, PMCID:PMC180726]
comment: The original formula for the gram stain consisted of aniline-gentian violet, iodine-potassium iodide, absolute alcohol, and sometimes bismark brown.
synonym: "gram staining evidence" EXACT []
is_a: ID:0001505 ! differential staining evidence
created_by: kvillanova
creation_date: 2015-06-19T14:54:03Z
[Term]
id: ID:0001514
name: microbiological medium evidence
def: "A type of microbiological assay evidence where a medium is used to culture and observe microorganisms." [ECO:KAV]
comment: Lab based media can come in various states such as solid, semisolid, or liquid.
synonym: "media evidence " EXACT []
synonym: "microbiological media evidence " EXACT []
is_a: ID:0001506 ! microbiological culture evidence
created_by: kvillanova
creation_date: 2015-06-19T14:58:07Z
[Term]
id: ID:0001515
name: undefined medium evidence
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-19T16:40:24Z
[Term]
id: ID:0001516
name: middlebrook 7h9 medium evidence
def: "A type of selective liquid medium evidence used in the growth and study of mycobacterium. The medium contains various growth factors including bovine albumin,dextrose,biotin,glycerol,sodium citrate,pyridoxine as well as other growth factors." [ECO:KAV]
synonym: "middlebrook 7h9 broth evidence" RELATED []
synonym: "middlebrook 7h9 media evidence" EXACT []
is_a: ID:0001539 ! selective liquid medium evidence
created_by: kvillanova
creation_date: 2015-06-22T11:54:48Z
[Term]
id: ID:0001517
name: semi-solid agar motility assay
def: "A type of motility assay evidence where bacterial cells are inoculated at the center of a semi-solid nutrient agar plate. After incubation in a appropriate temperature cells grow outward from the point of inoculation." [PMID:2674941, PMID:4957395]
is_a: ID:0001519 ! motility assay evidence
created_by: kvillanova
creation_date: 2015-06-22T12:46:04Z
[Term]
id: ID:0001518
name: brain-heart infusion blood medium evidence
def: "A type of blood medium evidence where extracts taken form brains, heats, and yeasts are combined with peptones to. Create a nonselective and enriched general purpose medium that can be used to grow a wide variety of organisms including many bacterial pathogens and fungi." [ECO:KAV, PMID:827553]
synonym: "brain-heart infusion evidence" EXACT []
synonym: "brain-heart infusion media evidence" EXACT []
is_a: ID:0001520 ! blood medium evidence
created_by: kvillanova
creation_date: 2015-06-22T15:02:51Z
[Term]
id: ID:0001519
name: motility assay evidence
is_a: ID:0001597 ! placeholder
created_by: kvillanova
creation_date: 2015-06-22T11:08:14Z
[Term]
id: ID:0001520
name: blood medium evidence
def: "A type of differential solid medium evidence that uses a enriched and differential media to test for a wide range of microbiological organisms. The medium commonly includes human, horse, or sheep blood cells as its main growth factor. This allows the media to be used to differentiate between alpha, gamma, and beta hemolysis causing bacteria." [ECO:KAV, PMID:16954271]
comment: A wide range of mammalian blood is used for blood agar. Examples include sheep,horse,pig,goat and human blood.
is_a: ID:0001544 ! differential solid medium evidence
is_a: ID:0001568 ! enriched solid medium evidence
created_by: kvillanova
creation_date: 2015-06-22T07:51:17Z
[Term]
id: ID:0001521
name: special staining evidence
synonym: "special stain evidence " RELATED []
is_a: ID:0001503 ! Place holder ( merge thease terms with) ECO:0005019 Staining evidence
created_by: kvillanova
creation_date: 2015-06-22T08:45:39Z
[Term]
id: ID:0001522
name: methylene blue staining evidence
def: "A type of simple stain evidence where methylene blue is used." [ECO:KAV]
synonym: "methylene blue staining evidence" EXACT []
is_a: ID:0001504 ! simple staining evidence
created_by: kvillanova
creation_date: 2015-06-22T08:46:19Z
[Term]
id: ID:0001523
name: endospore staining evidence
def: "A type of differential staining evidence based on any type of stain used to view bacterial endospores." [ECO:KAV]
synonym: "spore stain evidence" EXACT []
is_a: ID:0001505 ! differential staining evidence
created_by: kvillanova
creation_date: 2015-06-22T08:53:27Z
[Term]
id: ID:0001524
name: capsule staining evidence
def: "A type of special staining evidence based on any staining technique that is used to view bacterial capsules." [ECO:KAV]
synonym: "capsule stain evidence " EXACT []
is_a: ID:0001521 ! special staining evidence
is_a: ID:0001524 ! capsule staining evidence
created_by: kvillanova
creation_date: 2015-06-22T09:03:58Z
[Term]
id: ID:0001525
name: flagellar staining evidence
def: "A type of special staining evidence based on any type of stain used to view flagella." [ECO:KAV]
synonym: "flagellar stain evidence" EXACT []
is_a: ID:0001521 ! special staining evidence
created_by: kvillanova
creation_date: 2015-06-22T09:03:00Z
[Term]
id: ID:0001526
name: fluorescent microscopy evidence
is_a: ID:0001508 ! microscopy evidence (place holder)
created_by: kvillanova
creation_date: 2015-06-22T15:04:16Z
[Term]
id: ID:0001527
name: Ziehl-Neelson acid-fast staining evidence
def: "A type of differential staining evidence where carbol- fuchsin is used as the primary stain and methylene blue is used as the counterstain. Heat is applied to the specimen after the carbol- fuchsin exposure then the specimen is decolorized before the methylene blue counterstain." [ECO:KAV, PMCID:PMC518143]
comment: With this stain type acid fast positive organisms stain pink/red while acid fast negative organisms stain blue.
synonym: "Ziehl-Neelson acid fast stain evidence" EXACT []
is_a: ID:0001502 ! acid-fast staining evidence
created_by: kvillanova
creation_date: 2015-06-22T15:04:24Z
[Term]
id: ID:0001528
name: Schaeffer-Fulton staining evidence
def: "A type of endospore staining evidence where a primary dye of malachite green and a counterstain of safranin are used. The malachite green dye is forced into the endospore by exposure to heat and steam. The specimen is decolorized and then counterstained." [ECO:KAV, PMCID:PMC241728]
comment: With this staining technique bacterial endospores stain green and all other bacteria stain red/pink.
synonym: "Schaeffer-Fulton stain evidence" EXACT []
is_a: ID:0001523 ! endospore staining evidence
created_by: kvillanova
creation_date: 2015-06-22T15:04:28Z
[Term]
id: ID:0001529
name: acridine orange histological staining evidence
def: "A type of histological staining evidence where cells are stained with acridine orange, a cell permeable, UV fluorescent dye that binds to nucleic acids." [PMID:6168652]
comment: The Acridine orange stain is used to detect various parasites including Trichomonas vaginalis.
synonym: "AO evidence " EXACT []
synonym: "AO stain evidence " EXACT []
synonym: "AO staining evidence " EXACT []
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-09T11:26:56Z
[Term]
id: ID:0001530
name: Kinyoun acid-fast staining evidence
def: "A type of acid-fast staining evidence where kinyoun based carbol-fuchsin phenol is used as the primary stain. Methylene blue is used as the counterstain, and acid alcohol is used as a decolorizer." [ECO:KAV]
comment: With this staining technique the acid fast bacteria is stained red/pink and acid fast negative bacteria is stained blue.
synonym: "Kinyoun stain evidence" EXACT []
is_a: ID:0001502 ! acid-fast staining evidence
created_by: kvillanova
creation_date: 2015-06-22T15:29:35Z
[Term]
id: ID:0001531
name: calcoflour-white histological staining evidence
def: "A type of histological staining evidence where the fluorochrome calcoflour- white, a flourescent whitening agent, is used to stain cellulose or chitin." [ECO:KAV, PMID:19233954]
comment: Calcoflour-white is commonly used to identify infections caused by yeasts and other fungi.
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-09T11:27:02Z
[Term]
id: ID:0001532
name: Moeller staining evidence
def: "A type of endospore staining evidence where. Carbol- fuchsin is used as a primary dye and methylene blue is used as a counterstain. Carbol-fuchsin is forced into the endospore with exposure to heat and steam. The specimen is then decolorized with sulfuric acid and counterstained with methylene blue." [ECO:KAV, PMID:17933713]
comment: With this staining technique bacterial endospores are stained red/pink while all other bacteria are stained blue.
synonym: "Moeller stain evidence" EXACT []
is_a: ID:0001523 ! endospore staining evidence
created_by: kvillanova
creation_date: 2015-06-23T11:34:44Z
[Term]
id: ID:0001533
name: Abbott staining evidence
def: "A type of endospore staining evidence where. Methylene blue is the primary stain and anilin- fuchsin is the counterstain. Methylene blue is forced into the endospore with steam and heat exposure. The specimen is then washed in water, decolorized with 95% alcohol that contains .2 to .3 % HCL. Counterstained with anilin-fuchsin, and washed with water." [ECO:KAV, PMCID:PMC1516947]
comment: With this staining technique bacterial endospores stain blue all other bacteria stain red.
synonym: "abbott staining evidence" NARROW []
is_a: ID:0001523 ! endospore staining evidence
created_by: kvillanova
creation_date: 2015-06-23T13:05:48Z
[Term]
id: ID:0001534
name: tergitol 7 modified moeller stain evidence
def: "A type of moeller stain evidence where. Alliquots of tergitol 7 are used in replacement of heating and steaming the specimen." [ECO:KAV, PMID:17933713]
comment: With this staining technique endospores are stained red/pink while all other bacteria are stained blue.
synonym: "tergitol 7 modified moeller staining evidence" EXACT []
is_a: ID:0001532 ! Moeller staining evidence
created_by: kvillanova
creation_date: 2015-06-23T13:09:55Z
[Term]
id: ID:0001535
name: light microscopy evidence
def: "A type of microscopy evidence based on any technique that uses visible light to display the morphology of a specimen." [ECO:KAV, PMID:NBK9839]
synonym: "optical microscopy evidence " EXACT []
is_a: ID:0001508 ! microscopy evidence (place holder)
created_by: kvillanova
creation_date: 2015-06-24T09:46:13Z
[Term]
id: ID:0001536
name: bright-field microscopy evidence
def: "A type of light microscopy evidence where a condensed light is transmitted directly through a specimen." []
is_a: ID:0001535 ! light microscopy evidence
created_by: kvillanova
creation_date: 2015-06-24T09:47:56Z
[Term]
id: ID:0001537
name: dark field microscopy evidence
is_a: ID:0001508 ! microscopy evidence (place holder)
created_by: kvillanova
creation_date: 2015-06-24T09:48:16Z
[Term]
id: ID:0001538
name: electron microscopy evidence
def: "A type of microbiological microscopy evidence where a high voltage beam of accelerated electrons are fired at a sample to. Obtain higher then light resolution images of the sample.\n\n\nMerge with ECO:0005033 if theres no definition." [ECO:KAV, PMID:NBK26880]
is_a: ID:0001508 ! microscopy evidence (place holder)
created_by: kvillanova
creation_date: 2015-06-24T09:48:41Z
[Term]
id: ID:0001539
name: selective liquid medium evidence
def: "A type of selective medium evidence based on any type of media in a liquid state." [ECO:KAV]
synonym: "broth evidence " EXACT []
synonym: "liquid media evidence" EXACT []
is_a: ID:0001501 ! selective medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:34:51Z
[Term]
id: ID:0001540
name: selective solid medium evidence
def: "A type of selective medium evidence based on any type of media in a solid state." []
synonym: "solid agar media evidence " RELATED []
is_a: ID:0001501 ! selective medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:35:01Z
[Term]
id: ID:0001541
name: selective semi-solid medium evidence
def: "A type of selective medium evidence based on any type of media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
synonym: "semi-solid selective media" EXACT []
is_a: ID:0001501 ! selective medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:35:29Z
[Term]
id: ID:0001542
name: differential semi-solid medium evidence
def: "A type of differential medium evidence based on any type of media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
synonym: "differential semi-solid media evidence" EXACT []
is_a: ECO:0001502 ! differential medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:37:45Z
[Term]
id: ID:0001543
name: differential liquid medium evidence
def: "A type of differential liquid medium evidence based on any type of media in a liquid state." []
synonym: "differential broth media evidence" EXACT []
is_a: ECO:0001502 ! differential medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:37:50Z
[Term]
id: ID:0001544
name: differential solid medium evidence
def: "A type of differential medium evidence based on any type of media in a solid state." []
synonym: "differential solid media evidence" EXACT []
is_a: ECO:0001502 ! differential medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:37:54Z
[Term]
id: ID:0001545
name: undefined solid medium evidence
def: "A type of undefined solid medium evidence based on any type of media in a solid state." []
is_a: ID:0001515 ! undefined medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:42:27Z
[Term]
id: ID:0001546
name: undefined liquid medium evidence
def: "A type of undefined medium evidence based on any type of media in a liquid state." []
is_a: ID:0001515 ! undefined medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:43:11Z
[Term]
id: ID:0001547
name: undefined semi-solid medium evidence
def: "A type of undefined medium evidence based on any type of media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
is_a: ID:0001515 ! undefined medium evidence
created_by: kvillanova
creation_date: 2015-06-24T15:43:30Z
[Term]
id: ID:0001548
name: Beilschowsky histological silver staining evidence
is_a: ID:0001603 ! Silver histological staining evidence
created_by: kvillanova
creation_date: 2015-07-09T15:41:20Z
[Term]
id: ID:0001549
name: enriched medium evidence
def: "A type of microbiological medium evidence where a medium contains complex growth and nutrient factors. Those nutrient factors support a wide range of microbial organisms but is specifically used to culture fastidious organisms." [ECO:KAV, PMCID:3793873]
synonym: "enriched media evidence" EXACT []
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-25T11:17:31Z
[Term]
id: ID:0001550
name: nutrient medium evidence
synonym: "nutrient media evidence" EXACT []
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-25T11:19:28Z
[Term]
id: ID:0001551
name: transport medium evidence
synonym: "transport media evidence" EXACT []
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-25T11:21:33Z
[Term]
id: ID:0001552
name: pure culture evidence
is_a: ID:0001506 ! microbiological culture evidence
created_by: kvillanova
creation_date: 2015-06-25T13:44:17Z
[Term]
id: ID:0001553
name: defined medium evidence
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-25T11:25:26Z
[Term]
id: ID:0001554
name: minimal medium evidence
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-25T13:39:18Z
[Term]
id: ID:0001555
name: cajal histological staining evidence
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-10T09:05:48Z
[Term]
id: ID:0001556
name: mixed culture evidence
is_a: ID:0001506 ! microbiological culture evidence
created_by: kvillanova
creation_date: 2015-06-25T13:44:39Z
[Term]
id: ID:0001557
name: wound culture
is_a: ID:0001506 ! microbiological culture evidence
created_by: kvillanova
creation_date: 2015-06-25T13:50:48Z
[Term]
id: ID:0001558
name: aerobic wound culture evidence
is_a: ID:0001557 ! wound culture
created_by: kvillanova
creation_date: 2015-06-25T13:51:52Z
[Term]
id: ID:0001559
name: anaerobic wound culture evidence
is_a: ID:0001557 ! wound culture
created_by: kvillanova
creation_date: 2015-06-25T13:52:24Z
[Term]
id: ID:0001560
name: aerobic culture evidence
is_a: ID:0001506 ! microbiological culture evidence
created_by: kvillanova
creation_date: 2015-06-25T13:53:05Z
[Term]
id: ID:0001561
name: anaerobic wound culture evidence
is_a: ID:0001506 ! microbiological culture evidence
created_by: kvillanova
creation_date: 2015-06-25T13:53:21Z
[Term]
id: ID:0001562
name: Macconkey medium evidence
def: "A type of differential and selective solid medium used for the differentiation and isolation of non fastidious gram negative organisms. The media was created by Alfred Theodore MacConkey M.D when he worked at The Royal Commission On Sewage Disposal in Liverpool England (1905). The modern version of the media contains peptones, lactose, bile salts, sodium chloride, neutral red, crystal violet, and water." []
comment: The media can be used to culture gram negative lactose and non lactose fermenting organisms, enteric organisms, and coliforms. The Bile salt agar and crystal violet dye inhibit gram positive organisms from growing. The neutral red dye turns pink if microorganisms are lactose fermenting.
synonym: "Macconkey media evidence" EXACT []
is_a: ID:0001540 ! selective solid medium evidence
is_a: ID:0001544 ! differential solid medium evidence
created_by: kvillanova
creation_date: 2015-06-29T13:58:15Z
[Term]
id: ID:0001563
name: reducing medium evidence
is_a: ID:0001514 ! microbiological medium evidence
created_by: kvillanova
creation_date: 2015-06-25T14:32:07Z
[Term]
id: ID:0001564
name: alician blue histological staining evidence
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-10T08:27:36Z
[Term]
id: ID:0001565
name: Columbia blood medium evidence
def: "A type of blood medium evidence based on formula design from Ellen et al of Columbia University. The media contains various growth factors including. Sheep, rabbit, or horse blood. A pancreatic digest of casein, a meat peptic digest, yeast extract, maize starch and sodium chloride." [ECO:KAV, PMID:9810657]
synonym: "columbia blood media evidence" EXACT []
is_a: ID:0001520 ! blood medium evidence
created_by: kvillanova
creation_date: 2015-06-26T08:46:20Z
[Term]
id: ID:0001566
name: Columbia medium evidence
def: "A type of enriched solid medium evidence based on formula design from Ellen et al of Columbia University. The media is used for general purpose cultivation of microorganisms and contains various growth factors including. Pancreatic casein digest,a meat peptic digest, heart pancreatic digest, yeast extract, maize starch, and sodium chloride." []
synonym: "CA media evidence" EXACT []
synonym: "columbia agar media evidence" EXACT []
is_a: ID:0001568 ! enriched solid medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:04:30Z
[Term]
id: ID:0001567
name: enriched liquid medium evidence
def: "A type of enriched liquid medium evidence based on any type of media in a liquid state." []
is_a: ID:0001549 ! enriched medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:16:09Z
[Term]
id: ID:0001568
name: enriched solid medium evidence
def: "A type of enriched medium evidence based on any type of media in a solid state." []
is_a: ID:0001549 ! enriched medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:16:32Z
[Term]
id: ID:0001569
name: enriched semi-solid medium evidence
def: "A type of enriched medium evidence based on any type of media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
is_a: ID:0001549 ! enriched medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:17:02Z
[Term]
id: ID:0001570
name: defined liquid medium evidence
def: "A type of defined liquid medium based on any type of media in a liquid state." []
is_a: ID:0001553 ! defined medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:17:25Z
[Term]
id: ID:0001571
name: defined solid medium evidence
def: "A type of defined medium evidence based on any type of media in a solid state." []
is_a: ID:0001553 ! defined medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:17:47Z
[Term]
id: ID:0001572
name: defined semi-solid medium evidence
def: "A type of defined medium evidence based on any media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
is_a: ID:0001553 ! defined medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:18:05Z
[Term]
id: ID:0001573
name: semi-solid transport medium evidence
def: "A type of transport medium evidence based on any type of media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
is_a: ID:0001551 ! transport medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:48:34Z
[Term]
id: ID:0001574
name: solid transport medium evidence
def: "A type of transport medium evidence based on any type of media in a solid state." []
is_a: ID:0001551 ! transport medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:48:54Z
[Term]
id: ID:0001575
name: Maneval's capsule staining evidence
def: "A type of capsule staining evidence where. Congo red and a combination of acetic acid and acid-fuchsin are used to view bacterial capsules. The staining procedure is to put droplets of congo red on a slide and mix with a sample from culture. The slide must air dry and not be heat fixed. The congo red is a PH indicator and it dries on the slide leaving a red background then manval's solution a mixture of acetic acid and acid-fuchsin is added to the slide. The acid-fuchsin stains the bacteria bright red and the acetic acid acts as a mordant." [ECO:KAV, PMID:19239855, PMID:23825728]
comment: Congo red is a pH indicator and changes color when exposed to different pH. It is blue below 3.0 pH and will turn red if exposed to a pH above 5.2. Due to this when a maneval's stain is done the slide shows red white and blue red and blue for the background while the bacteria is stained red by the acid fuchsin the capsule remains white. The slide must be rinsed at the end and not blot dried because of the same reason it cannot be heat fixed because those actions would destroy or damage the destroy the capsule.
synonym: "maneval's capsule staining evidence" EXACT []
synonym: "maneval's staining evidence" RELATED []
is_a: ID:0001524 ! capsule staining evidence
created_by: kvillanova
creation_date: 2015-06-26T14:40:26Z
[Term]
id: ID:0001576
name: Anthony's capsule staining evidence
def: "A type of capsule stain evidence that uses crystal violet to view bacterial capsules. No counterstain is necessary for this type of stain. Only a 20% solution of copper sulfate is used as a mordant after the crystal violet is applied." [ECO:KAV]
comment: The slide cannot be heat fixed or blot dried because that action would destroy or damage the bacterial capsule. The background of the slide is stained purple while the capsules show up white.
synonym: "anthony's capsule staining evidence" NARROW []
synonym: "anthony's stain evidence" RELATED []
synonym: "anthony's staining evidence" EXACT []
is_a: ID:0001524 ! capsule staining evidence
created_by: kvillanova
creation_date: 2015-06-26T14:42:03Z
[Term]
id: ID:0001577
name: liquid nutrient medium evidence
def: "A type of liquid nutrient medium evidence based on any type of media in a liquid state." []
is_a: ID:0001550 ! nutrient medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:38:17Z
[Term]
id: ID:0001578
name: solid nutrient medium evidence
def: "A type of solid nutrient medium evidence based on any type of media in a solid state." []
is_a: ID:0001550 ! nutrient medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:39:30Z
[Term]
id: ID:0001579
name: semi-solid nutrient medium evidence
def: "A type of nutrient medium evidence based on any type of media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
is_a: ID:0001550 ! nutrient medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:40:02Z
[Term]
id: ID:0001580
name: solid reducing medium evidence
def: "A type of reducing medium evidence based on any type of media in a solid state." []
is_a: ID:0001563 ! reducing medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:40:46Z
[Term]
id: ID:0001581
name: liquid reducing medium evidence
def: "A type of liquid reducing medium based on any type of media in a liquid state." []
is_a: ID:0001563 ! reducing medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:41:19Z
[Term]
id: ID:0001582
name: semi-solid reducing medium evidence
def: "A type of reducing medium evidence based on any type of media in a semi-solid state." []
comment: Semi-solid media is considered to be media that is less concentrated generally 0.5 % agar content or less.
is_a: ID:0001563 ! reducing medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:41:37Z
[Term]
id: ID:0001583
name: minimal solid medium evidence
def: "A type of minimal medium evidence based on any type of media in a solid state." []
is_a: ID:0001554 ! minimal medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:43:28Z
[Term]
id: ID:0001584
name: minimal semi-solid medium evidence
def: "A type of minimal medium evidence based on any type of media in a semi-solid state." []
is_a: ID:0001554 ! minimal medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:43:52Z
[Term]
id: ID:0001585
name: liquid transport medium evidence
def: "A type of transport medium evidence based on any type of media in a liquid state." []
is_a: ID:0001551 ! transport medium evidence
created_by: kvillanova
creation_date: 2015-06-26T10:46:52Z
[Term]
id: ID:0001586
name: China blue lactose medium evidence
def: "A type of differential solid medium evidence used to differentiate between lactose fermenting and non lactose fermenting microorganisms. The media serves as a PH indicator and can be incubated with milk. It has various growth factors including a casein peptone, lactose, meat extract, and sodium chloride." []
synonym: "china blue agar evidence" EXACT []
synonym: "china blue evidence" EXACT []
is_a: ID:0001544 ! differential solid medium evidence
created_by: kvillanova
creation_date: 2015-06-26T15:05:23Z
[Term]
id: ID:0001587
name: catalase assay evidence
def: "A type of microbiological biochemical evidence based on any assay that is used to determine if an organism produces the enzyme catalase. The test works by adding a small amount of hydrogen peroxide to a microbial sample. If the enzyme catalase is present it breaks down the hydrogen peroxide forming water and oxygen gas. The reaction is observed as a formation of bubbles." [ECO:KAV, PMCID:PMC379865]
is_a: ID:0001509 ! microbiological biochemical evidence
created_by: kvillanova
creation_date: 2015-06-29T07:47:08Z
[Term]
id: ID:0001588
name: coagulase assay evidence
def: "A type of microbiological biochemical evidence based on any assay that determines if a bacterium can coagulate EDTA treated mammalian plasma. The coagulase test is usually used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. However it has been shown that other microorganisms can coagulate mammalian plasma as well. The test works due bacterial protein production of coagulase which allows fibrinogen to be converted to fibrin." [ECO:KAV, PMID:14255647, PMID:18905247, PMID:2185284, PMID:6808014]
comment: There are two subtypes of coagulase test one is done on a slide the other is done in a tube. The slide can give fast results, but often false positives. The tube can take up to 24 hours for results but is generally more accurate.
is_a: ID:0001509 ! microbiological biochemical evidence
created_by: kvillanova
creation_date: 2015-06-29T07:48:33Z
[Term]
id: ID:0001589
name: Spirit blue medium evidence
def: "A type of differential solid medium evidence used to differentiate organisms that produce lipase. The media was created by Starr in 1941, other medias of the time did not allow for the growth of certain organisms because the types of dyes used was too toxic. Starr solved this by using spirit blue as the base dye. The agar media also contain a lipase reagent a mixture of tributyrin and polysorbate 80 as well as yeast extract, B-complex vitamins, and peptone as a source of carbon , nitrogen, and other vitamin's." [ECO:KAV, PMID:17748334, PMID:24274015]
comment: The original media formula consisted of 30g agar, 10g tryptone, 5m yeast extract, 250 ml of 20% cotton seed oil emulsion, 50 ml of 0.3 % alcholic solution of spirit blue, and 1000 ml of distilled water to make. Other lipodial emulsions can be created by using meal, cream , vegetable oil, or olive oil.
synonym: "spirit blue evidence " EXACT []
synonym: "spirit blue media evidence " EXACT []
is_a: ID:0001544 ! differential solid medium evidence
created_by: kvillanova
creation_date: 2015-06-29T08:09:46Z
[Term]
id: ID:0001590
name: fermentation assay evidence
def: "A type of microbiological biochemical evidence based on any assay used to detect fermentation." [ECO:KAV]
is_a: ID:0001509 ! microbiological biochemical evidence
created_by: kvillanova
creation_date: 2015-06-29T08:13:26Z
[Term]
id: ID:0001591
name: transmission electron microscopy evidence
def: "A type of electron microscopy evidence based on a focused electron wavelength that is transmitted through a thin specimen. The electrons transmitted through the specimen hold information about the structure of the specimen. Which is magnified through a series of lenses and is recorded on a fluorescent plate or a sensor. The resulting image is able to show details in high resolution at the picometer size level." [ECO:KAV, PMID:24482357]
is_a: ID:0001538 ! electron microscopy evidence
created_by: kvillanova
creation_date: 2015-06-29T09:12:24Z
[Term]
id: ID:0001592
name: cryo-electron microscopy evidence
def: "A type of transmission electron microscopy evidence where thin samples are flash frozen." [ECO:KAV, PMID:23181775, PMID:25651412]
synonym: "cryo-EM evidence " EXACT []
is_a: ID:0001591 ! transmission electron microscopy evidence
created_by: kvillanova
creation_date: 2015-06-29T09:14:03Z
[Term]
id: ID:0001593
name: fluroscent acid-fast microscopy evidence
def: "A type of fluroscent microscopy where a acid-fast stain is used." [PMCID:PMC93186]
is_a: ID:0001526 ! fluorescent microscopy evidence
created_by: kvillanova
creation_date: 2015-06-29T10:59:12Z
[Term]
id: ID:0001594
name: oxidase assay evidence
def: "A type of microbiological biochemical assay evidence based on any assay used to determine if a type of microbe produces cytochrome oxidase. The test works by oxidizing a reagent called tetramethyl-p-phenylenediamine turning it purple. If oxidase is not present in the bacterium the reagent stays colorless." [ECO:KAV, PMCID:PMC291174]
is_a: ID:0001509 ! microbiological biochemical evidence
created_by: kvillanova
creation_date: 2015-06-29T11:12:21Z
[Term]
id: ID:0001595
name: buffered charcoal yeast extract medium evidence
def: "A type of selective solid agar medium evidence originally used for the cultivation of the Legionella species. The agar media contains yeast extracts, charcoal activated, ACES buffer, l-cysteine hcl, and a-ketoglutarate monopotassium salt." [ECO:KAV, PMID:2116452, PMID:393713, PMID:7009642, PMID:9780107, PMID:PMC265402]
comment: The media has been modified many times over the years. Legionella pneumophila was first discovered in 1977 by MacDade et al. In 1979 Feeley et al developed a medium that provided consistent isolation of Legionella. He later modified that media by replacing casein hydrolase with yeast and beef extract. In 1980 Pasculle et al added the ACES buffer giving the media the designation BCYE ( buffered charcoal yeast extract). During 1981 Edelstien et al. Increased the sensitivity of the media by adding alpha-ketoglutaric potsasium salt. During that same year the media was made even more selective for Legionella by Wadowsky and Yee by adding the antibiotics glycine, vancomycin, and polymyxin. The latest modification was made in 1984 by Dennis et al. By adding cycloheximide creating GVPC medium. \n\nIn recent studies the agar media has been shown the ability to culture other species of microorganisms including Brucellae, Nocardia and Acanthamoeba keratitis.
synonym: "buffered charcoal yeast extract medium evidence" EXACT []
synonym: "BYCE media evidence" EXACT []
is_a: ID:0001540 ! selective solid medium evidence
created_by: kvillanova
creation_date: 2015-06-29T15:16:21Z
[Term]
id: ID:0001596
name: Romanowsky histological staining evidence
synonym: "romanowsky staining evidence" EXACT []
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-06-30T08:24:49Z
[Term]
id: ID:0001597
name: placeholder
def: "Just a lonely place holder." []
created_by: kvillanova
creation_date: 2015-06-30T12:51:58Z
[Term]
id: ID:0001598
name: phase-contrast microscopy evidence
is_a: ID:0001508 ! microscopy evidence (place holder)
created_by: kvillanova
creation_date: 2015-06-30T10:10:41Z
[Term]
id: ID:0001599
name: differential interference-contrast microscopy evidence
is_a: ID:0001508 ! microscopy evidence (place holder)
created_by: kvillanova
creation_date: 2015-06-30T10:10:58Z
[Term]
id: ID:0001600
name: immunoelectron microscopy evidence
is_a: ID:0001538 ! electron microscopy evidence
created_by: kvillanova
creation_date: 2015-06-30T10:31:19Z
[Term]
id: ID:0001601
name: India ink capsule staining evidence
def: "A type of capsule staining evidence that uses india ink to view bacterial capsules." []
comment: The india ink stains the background black causing the bacterial capsules to appear white.
synonym: "India ink staining evidence" EXACT []
synonym: "India stain evidence " EXACT []
is_a: ID:0001524 ! capsule staining evidence
created_by: kvillanova
creation_date: 2015-07-01T10:51:51Z
[Term]
id: ID:0001602
name: Grocott's methenamine histological silver staining evidence
synonym: "GMS stain evidence" EXACT []
is_a: ID:0001603 ! Silver histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T11:31:38Z
[Term]
id: ID:0001603
name: Silver histological staining evidence
def: "A type of histological staining evidence based on any type of stain that uses silver to stain a specimen." []
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T11:33:05Z
[Term]
id: ID:0001604
name: Warthin-Starry histological staining evidence
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T11:33:59Z
[Term]
id: ID:0001605
name: Leishman histological staining evidence
is_a: ID:0001596 ! Romanowsky histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:40:24Z
[Term]
id: ID:0001606
name: Wright's histological staining evidence
is_a: ID:0001596 ! Romanowsky histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:40:54Z
[Term]
id: ID:0001607
name: field histological staining evidence
is_a: ID:0001596 ! Romanowsky histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:41:10Z
[Term]
id: ID:0001608
name: Jenner's histological staining evidence
is_a: ID:0001596 ! Romanowsky histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:42:02Z
[Term]
id: ID:0001609
name: gimsa histological staining evidence
is_a: ID:0001596 ! Romanowsky histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:42:36Z
[Term]
id: ID:0001610
name: periodic acid-schiff histological staining evidence
def: "A type of histological staining evidence based on a reaction where, periodic acid oxidizes substances that contain hydroxl groups and the product of the reaction reats with Schiffs reagent forming a megenta color." []
synonym: "PAS stain evidence " EXACT []
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:42:56Z
[Term]
id: ID:0001611
name: hematoxylin and eosin histological staining evidence
def: "A type of histology staining evidence based on a type of stain that uses Hematoxylin as a primary stain and Eosin as a counterstain. The Hematoxylin stains the nucleic material while the Eosin stains the cytoplasmic material." [ECO:KAV, PMID:24406626]
comment: With this staining technique nucleic materials are stained a dark purple/blue. Cytoplasmic materials are stained pink/red.
synonym: "H&E stain evidence" EXACT []
synonym: "H&E staining evidence" EXACT []
synonym: "HE stain evidence" RELATED []
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:45:03Z
[Term]
id: ID:0001612
name: Nissl histological staining evidence
def: "A type of histological staining evidence" []
synonym: "cresyl violet staining evidence" EXACT []
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:47:35Z
[Term]
id: ID:0001613
name: Macchiavello histological staining evidence
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:49:36Z
[Term]
id: ID:0001614
name: lendrums phloxine-tartazine histological staining evidence
is_a: ID:0001617 ! histological staining evidence
created_by: kvillanova
creation_date: 2015-07-01T12:49:58Z
[Term]
id: ID:0001615
name: New York City medium evidence
def: "A type of blood agar medium evidence designed for the cultivation of pathogenic species of Neisseria. The agar media was created by Fauer, Weisburd, and Wilson while working at the New York city department of health. It contains proteose peptone, corns starch, glucose, sodium chloride, diopatassium hydrogen phosphate, potsassium hydrogen phosphate, hourse plasma , hemoglobin, dextrose, and agar." [ECO:KAV, PMID:4208637, PMID:6439732, PMID:6796599]
comment: The selective supplements commonly used to select for the growth of Neisseria added to the media are vancomycin, colistin, nystatin, and trimethoprim. The media is capable of supporting other microorganisms including mycoplasmas and T-mycoplasmas.
is_a: ID:0001520 ! blood medium evidence
created_by: kvillanova
creation_date: 2015-07-01T13:05:03Z
[Term]
id: ID:0001616
name: chocolate blood medium evidence
def: "A type of blood medium evidence used for the isolation of pathogenic bacteria. The medium contains specific enzyme growth factors including NAD, hemin, l-cysteine hcl, l-gultamine, adenine and. The general make up of the media usually includes preteose peptone, sodium chloride, diopatassium phosphate, monopotassium phosphate, corn starch, horse blood, and agar." [ECO:KAV, PMID:16561550, PMID:6490866]
comment: This media does not contain any chocolate it is only named so because of its color. The media is most recognized for its ability to sustain Hemaophilus and Nisseria bacterial strains.
synonym: "CBA media evidence" EXACT []
synonym: "CBA medium evidence" EXACT []
synonym: "chocolate blood media evidence" EXACT []