From 2eaefc9dc90c61c3bab652a19695f4ac8e12e516 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 3 Mar 2023 17:01:28 -0800 Subject: [PATCH 01/16] flowchart elements created --- docs/pipeline_flowchart.puml | 31 ++++++++++++++++++++++++++++++- 1 file changed, 30 insertions(+), 1 deletion(-) diff --git a/docs/pipeline_flowchart.puml b/docs/pipeline_flowchart.puml index 0a5f2fc6..d6bf2b03 100644 --- a/docs/pipeline_flowchart.puml +++ b/docs/pipeline_flowchart.puml @@ -1,4 +1,3 @@ -@startuml muse_chart !include pipeline_elements.iuml!string_functions !include pipeline_elements.iuml!input_rect @@ -9,6 +8,8 @@ skinparam linetype ortho +@startuml muse_chart + $input_process(normal_bam, 'Normal bam', 'input normal bam file') $input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') @@ -24,3 +25,31 @@ dbsnp_file -d-> muse_sump_run $add_legend("left") @enduml + + +@startuml mutect2_chart + +$input_process(normal_bam, 'Normal bam', 'input normal bam file') +$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') +$input_process(contamination_table, 'Contamination Table', 'output of GATK\'s CalculateContamination') +$input_process(reference_genome', 'Reference genome, 'fasta file') +$input_process(germline_resource', 'Germline resource, 'gnomad vcf') +$input_process(germline_resource', 'Germline resource, 'gnomad vcf') + +$intermediate_process(gatk_splitIntervals, "gatk SplitIntervals", "Output: reference genome intervals %newline()# = scattercount") +$intermediate_process(mutect2_call_sSNV, "gatk Mutect2", "Output: unfiltered vcf(s), f1r2 file(s), stats file(s)") +$intermediate_process(gatk_mergeVcfs, "gatk MergeVcfs", "Output: unfiltered vcf") +$intermediate_process(mutect2_mergeStats, "gatk MergeMutectStats", "Output: Mutect2 stats") +$intermediate_process(gatk_learnReadOrientation, "gatk LearnReadOrientationModel", "Output: read-orientation-model") +$intermediate_process(mutect2_filterCalls, "gatk FilterMutectCalls", "Output: gatk filtered vcf, filtering stats") +$output_process(awk_filterVCF, "remove non-PASSing variants", "Output: filtered vcf") + +reference_genome -d-> gatk_splitIntervals +tumor_bam -d-> muse_call_run +normal_bam -d-> muse_call_run +muse_call_run -d-> muse_sump_run +dbsnp_file -d-> muse_sump_run + +$add_legend("left") + +@enduml From 83c33a329d6ede56f834ece53974d56797bb743d Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Wed, 8 Mar 2023 19:09:30 -0800 Subject: [PATCH 02/16] README pipeline steps correction (non-canonical are not scattered) and add mutect flowchart elements --- README.md | 14 ++++++-------- docs/pipeline_flowchart.puml | 34 +++++++++++++++++----------------- 2 files changed, 23 insertions(+), 25 deletions(-) diff --git a/README.md b/README.md index d602cbd9..218bb22f 100644 --- a/README.md +++ b/README.md @@ -141,19 +141,17 @@ The input pair of tumor/normal bam files, along with the candidate small indel f ### GATK Mutect 2 #### 1. Intervals not provided - ##### a. Split non-canonical - Split the set of non-canonical chromosomes into x intervals for parallelization, where x is defined by the input scatter_count. - ##### b. Call non-canonical + ##### a. Call non-canonical Call somatic variants in non-canonical chromosomes with `Mutect2`. - ##### c. Split canonical + ##### b. Split canonical Split the set of canonical chromosomes into x intervals for parallelization, where x is defined by the input `params.scatter_count`. - ##### d. Call canonical + ##### c. Call canonical Call somatic variant in canonical chromosomes with `Mutect2`. - ##### e. Merge + ##### d. Merge Merge scattered canonical and non-canonical chromosome outputs (vcfs, statistics). - ##### f. Learn read orientations + ##### e. Learn read orientations Create artifact prior table based on read orientations with GATK's `LearnReadOrientationModel`. - ##### g. Filter + ##### f. Filter Filter variants with GATK's `FilterMutectCalls`, using read orientation prior table and contamination table as well as standard filters. #### 2. Intervals provided diff --git a/docs/pipeline_flowchart.puml b/docs/pipeline_flowchart.puml index d6bf2b03..ec13d3d6 100644 --- a/docs/pipeline_flowchart.puml +++ b/docs/pipeline_flowchart.puml @@ -13,16 +13,16 @@ skinparam linetype ortho $input_process(normal_bam, 'Normal bam', 'input normal bam file') $input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') -$intermediate_process(muse_call_run, "MuSE call", "Output: position-specific %newline()summary statistics") -$input_process(dbsnp_file, 'dbSNP', 'dbSNP vcf') -$output_process(muse_sump_run, "MuSE sump", "Output: tier-based %newline()cutoffs") +$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') +$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') +$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') tumor_bam -d-> muse_call_run normal_bam -d-> muse_call_run muse_call_run -d-> muse_sump_run dbsnp_file -d-> muse_sump_run -$add_legend("left") +$add_legend('left') @enduml @@ -31,18 +31,18 @@ $add_legend("left") $input_process(normal_bam, 'Normal bam', 'input normal bam file') $input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') -$input_process(contamination_table, 'Contamination Table', 'output of GATK\'s CalculateContamination') -$input_process(reference_genome', 'Reference genome, 'fasta file') -$input_process(germline_resource', 'Germline resource, 'gnomad vcf') -$input_process(germline_resource', 'Germline resource, 'gnomad vcf') - -$intermediate_process(gatk_splitIntervals, "gatk SplitIntervals", "Output: reference genome intervals %newline()# = scattercount") -$intermediate_process(mutect2_call_sSNV, "gatk Mutect2", "Output: unfiltered vcf(s), f1r2 file(s), stats file(s)") -$intermediate_process(gatk_mergeVcfs, "gatk MergeVcfs", "Output: unfiltered vcf") -$intermediate_process(mutect2_mergeStats, "gatk MergeMutectStats", "Output: Mutect2 stats") -$intermediate_process(gatk_learnReadOrientation, "gatk LearnReadOrientationModel", "Output: read-orientation-model") -$intermediate_process(mutect2_filterCalls, "gatk FilterMutectCalls", "Output: gatk filtered vcf, filtering stats") -$output_process(awk_filterVCF, "remove non-PASSing variants", "Output: filtered vcf") +$input_process(contamination_table, 'Contamination Table', 'output of GATK CalculateContamination') +$input_process(reference_genome, 'Reference genome', 'fasta file') +$input_process(germline_resource, 'Germline resource', 'gnomad VCF') + + +$intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome intervals %newline()# = scattercount') +$intermediate_process(mutect2_call_sSNV, 'GATK Mutect2', 'Output: unfiltered VCF(s), f1r2 file(s), stats file(s)') +$intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') +$intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats') +$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: read-orientation-model') +$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF, filtering stats') +$output_process(awk_filterVCF, 'remove non-PASSing variants', 'Output: filtered VCF') reference_genome -d-> gatk_splitIntervals tumor_bam -d-> muse_call_run @@ -50,6 +50,6 @@ normal_bam -d-> muse_call_run muse_call_run -d-> muse_sump_run dbsnp_file -d-> muse_sump_run -$add_legend("left") +$add_legend('left') @enduml From 352e3a3934bbea05dd7c71d5dc895afaa678f5ea Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Thu, 9 Mar 2023 17:35:11 -0800 Subject: [PATCH 03/16] flowchart elements connected --- README.md | 2 +- docs/pipeline_flowchart.puml | 32 ++++++++++++++++++++++---------- 2 files changed, 23 insertions(+), 11 deletions(-) diff --git a/README.md b/README.md index 218bb22f..99dfd7e9 100644 --- a/README.md +++ b/README.md @@ -156,7 +156,7 @@ The input pair of tumor/normal bam files, along with the candidate small indel f #### 2. Intervals provided ##### a. Split - Split the set of provided intervals into x intervals for parallelization, where x is defined by the input scatter count. + Split the set of provided intervals into x intervals for parallelization, where x is defined by the input `params.scatter_count`. ##### b. Call Call somatic variants for the provided intervals with `Mutect2`. ##### c. Merge diff --git a/docs/pipeline_flowchart.puml b/docs/pipeline_flowchart.puml index ec13d3d6..96f8204a 100644 --- a/docs/pipeline_flowchart.puml +++ b/docs/pipeline_flowchart.puml @@ -31,24 +31,36 @@ $add_legend('left') $input_process(normal_bam, 'Normal bam', 'input normal bam file') $input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') -$input_process(contamination_table, 'Contamination Table', 'output of GATK CalculateContamination') +$input_process(contamination_table, 'Contamination Table: optional', 'output of GATK %newline()CalculateContamination') $input_process(reference_genome, 'Reference genome', 'fasta file') $input_process(germline_resource, 'Germline resource', 'gnomad VCF') -$intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome intervals %newline()# = scattercount') -$intermediate_process(mutect2_call_sSNV, 'GATK Mutect2', 'Output: unfiltered VCF(s), f1r2 file(s), stats file(s)') +$intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome split into %newline()params.scatter_count intervals') +$intermediate_process(mutect2_call_sSNV, 'GATK Mutect2: one process per interval', 'Output: unfiltered VCFs, variant stats, F1R2 metrics') $intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') $intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats') -$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: read-orientation-model') -$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF, filtering stats') -$output_process(awk_filterVCF, 'remove non-PASSing variants', 'Output: filtered VCF') +$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: orientation bias artifacts table') +$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF %newline()Optional output: filtering stats') +$output_process(awk_filterVCF, 'remove non-PASS variants', 'Output: filtered-pass.vcf.gz') +$qc_process(gatk_filteringStats, 'Optional', 'Output: filteringStats.tsv') reference_genome -d-> gatk_splitIntervals -tumor_bam -d-> muse_call_run -normal_bam -d-> muse_call_run -muse_call_run -d-> muse_sump_run -dbsnp_file -d-> muse_sump_run +normal_bam -d-> mutect2_call_sSNV +tumor_bam -d-> mutect2_call_sSNV +gatk_splitIntervals -d-> mutect2_call_sSNV +germline_resource -d-> mutect2_call_sSNV +mutect2_call_sSNV -d-> gatk_mergeVcfs +mutect2_call_sSNV -d-> gatk_learnReadOrientation +mutect2_call_sSNV -d-> mutect2_mergeStats + +gatk_mergeVcfs -d-> mutect2_filterCalls : // unfiltered.vcf.gz // +gatk_learnReadOrientation -d-> mutect2_filterCalls : // f1r2.tar.gz // +mutect2_mergeStats -d-> mutect2_filterCalls : // unfiltered.vcf.stats.gz // +reference_genome -d-> mutect2_filterCalls +contamination_table -d-> mutect2_filterCalls +mutect2_filterCalls -d-> gatk_filteringStats +mutect2_filterCalls -d-> awk_filterVCF : // filtered.vcf.gz // $add_legend('left') From ae040dda3f54e3b7f6bad48161cc163705d1b49c Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Thu, 9 Mar 2023 17:55:27 -0800 Subject: [PATCH 04/16] split muse and mutect into two .puml --- CHANGELOG.md | 1 + docs/muse_flowchart.puml | 27 +++++++++++++++++++ ..._flowchart.puml => mutect2_flowchart.puml} | 19 ------------- 3 files changed, 28 insertions(+), 19 deletions(-) create mode 100644 docs/muse_flowchart.puml rename docs/{pipeline_flowchart.puml => mutect2_flowchart.puml} (81%) diff --git a/CHANGELOG.md b/CHANGELOG.md index 69b0c6dc..ac368cb1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -6,6 +6,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## [Unreleased] ### Added +- Add Mutect2 flow chart - Add plantUML action and MuSE flow chart - Add NF-test ### Changed diff --git a/docs/muse_flowchart.puml b/docs/muse_flowchart.puml new file mode 100644 index 00000000..2254b189 --- /dev/null +++ b/docs/muse_flowchart.puml @@ -0,0 +1,27 @@ + +!include pipeline_elements.iuml!string_functions +!include pipeline_elements.iuml!input_rect +!include pipeline_elements.iuml!output_rect +!include pipeline_elements.iuml!intermediate_rect +!include pipeline_elements.iuml!qc_rect +!include pipeline_elements.iuml!process_legend + +skinparam linetype ortho + +@startuml muse_chart + +$input_process(normal_bam, 'Normal bam', 'input normal bam file') +$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') + +$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') +$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') +$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') + +tumor_bam -d-> muse_call_run +normal_bam -d-> muse_call_run +muse_call_run -d-> muse_sump_run +dbsnp_file -d-> muse_sump_run + +$add_legend('left') + +@enduml diff --git a/docs/pipeline_flowchart.puml b/docs/mutect2_flowchart.puml similarity index 81% rename from docs/pipeline_flowchart.puml rename to docs/mutect2_flowchart.puml index 96f8204a..c810785a 100644 --- a/docs/pipeline_flowchart.puml +++ b/docs/mutect2_flowchart.puml @@ -8,25 +8,6 @@ skinparam linetype ortho -@startuml muse_chart - -$input_process(normal_bam, 'Normal bam', 'input normal bam file') -$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') - -$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') -$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') -$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') - -tumor_bam -d-> muse_call_run -normal_bam -d-> muse_call_run -muse_call_run -d-> muse_sump_run -dbsnp_file -d-> muse_sump_run - -$add_legend('left') - -@enduml - - @startuml mutect2_chart $input_process(normal_bam, 'Normal bam', 'input normal bam file') From 42f9bb9bd2fcd07ef754027dbcaf5b75522ab729 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Thu, 9 Mar 2023 18:03:59 -0800 Subject: [PATCH 05/16] add mutect flow chart to README --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 99dfd7e9..e32c64c2 100644 --- a/README.md +++ b/README.md @@ -93,7 +93,7 @@ Version: v2.9.10 (Released on Nov 7, 2018) GitHub Package: ghcr.io/uclahs-cds/strelka2:2.9.10 ### Mutect 2 - +![alt text](docs/mutect2_chart.svg?raw=true) #### Tools ##### GATK GATK source: https://github.com/broadinstitute/gatk From c22041fe8dd59628a131c95e34f799ca6a49d031 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 09:51:06 -0800 Subject: [PATCH 06/16] testing plantUML action: removed old chart --- docs/muse_chart.svg | 96 --------------------------------------------- 1 file changed, 96 deletions(-) delete mode 100644 docs/muse_chart.svg diff --git a/docs/muse_chart.svg b/docs/muse_chart.svg deleted file mode 100644 index fa008400..00000000 --- a/docs/muse_chart.svg +++ /dev/null @@ -1,96 +0,0 @@ -MusEMuSE callOutput: position-specificsummary statisticsdbSNPdbSNP vcfMuSE sumpOutput: tier-basedcutoffsNormal baminput normal bam fileTumor baminput tumor bam fileColorProcess Type Input Intermediate Output QC \ No newline at end of file From b8f5ca7ce58dd7506e201ccede172dba4dea873f Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 10:07:06 -0800 Subject: [PATCH 07/16] testing plantUML action: use name pipeline_flowchart.puml --- docs/mutect2_flowchart.puml | 48 ------------------------------------- 1 file changed, 48 deletions(-) delete mode 100644 docs/mutect2_flowchart.puml diff --git a/docs/mutect2_flowchart.puml b/docs/mutect2_flowchart.puml deleted file mode 100644 index c810785a..00000000 --- a/docs/mutect2_flowchart.puml +++ /dev/null @@ -1,48 +0,0 @@ - -!include pipeline_elements.iuml!string_functions -!include pipeline_elements.iuml!input_rect -!include pipeline_elements.iuml!output_rect -!include pipeline_elements.iuml!intermediate_rect -!include pipeline_elements.iuml!qc_rect -!include pipeline_elements.iuml!process_legend - -skinparam linetype ortho - -@startuml mutect2_chart - -$input_process(normal_bam, 'Normal bam', 'input normal bam file') -$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') -$input_process(contamination_table, 'Contamination Table: optional', 'output of GATK %newline()CalculateContamination') -$input_process(reference_genome, 'Reference genome', 'fasta file') -$input_process(germline_resource, 'Germline resource', 'gnomad VCF') - - -$intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome split into %newline()params.scatter_count intervals') -$intermediate_process(mutect2_call_sSNV, 'GATK Mutect2: one process per interval', 'Output: unfiltered VCFs, variant stats, F1R2 metrics') -$intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') -$intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats') -$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: orientation bias artifacts table') -$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF %newline()Optional output: filtering stats') -$output_process(awk_filterVCF, 'remove non-PASS variants', 'Output: filtered-pass.vcf.gz') -$qc_process(gatk_filteringStats, 'Optional', 'Output: filteringStats.tsv') - -reference_genome -d-> gatk_splitIntervals -normal_bam -d-> mutect2_call_sSNV -tumor_bam -d-> mutect2_call_sSNV -gatk_splitIntervals -d-> mutect2_call_sSNV -germline_resource -d-> mutect2_call_sSNV -mutect2_call_sSNV -d-> gatk_mergeVcfs -mutect2_call_sSNV -d-> gatk_learnReadOrientation -mutect2_call_sSNV -d-> mutect2_mergeStats - -gatk_mergeVcfs -d-> mutect2_filterCalls : // unfiltered.vcf.gz // -gatk_learnReadOrientation -d-> mutect2_filterCalls : // f1r2.tar.gz // -mutect2_mergeStats -d-> mutect2_filterCalls : // unfiltered.vcf.stats.gz // -reference_genome -d-> mutect2_filterCalls -contamination_table -d-> mutect2_filterCalls -mutect2_filterCalls -d-> gatk_filteringStats -mutect2_filterCalls -d-> awk_filterVCF : // filtered.vcf.gz // - -$add_legend('left') - -@enduml From bb6d0c99fac7ab745c9e874c9e14a350b1c5996c Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 10:12:42 -0800 Subject: [PATCH 08/16] testing plantUML action: remove second .puml file --- docs/muse_flowchart.puml | 27 --------------------------- 1 file changed, 27 deletions(-) delete mode 100644 docs/muse_flowchart.puml diff --git a/docs/muse_flowchart.puml b/docs/muse_flowchart.puml deleted file mode 100644 index 2254b189..00000000 --- a/docs/muse_flowchart.puml +++ /dev/null @@ -1,27 +0,0 @@ - -!include pipeline_elements.iuml!string_functions -!include pipeline_elements.iuml!input_rect -!include pipeline_elements.iuml!output_rect -!include pipeline_elements.iuml!intermediate_rect -!include pipeline_elements.iuml!qc_rect -!include pipeline_elements.iuml!process_legend - -skinparam linetype ortho - -@startuml muse_chart - -$input_process(normal_bam, 'Normal bam', 'input normal bam file') -$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') - -$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') -$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') -$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') - -tumor_bam -d-> muse_call_run -normal_bam -d-> muse_call_run -muse_call_run -d-> muse_sump_run -dbsnp_file -d-> muse_sump_run - -$add_legend('left') - -@enduml From d316f99b915a54719d03a90f47d5547755e42e22 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 10:24:45 -0800 Subject: [PATCH 09/16] testing plantUML action: re-add pipeline_flowchart.puml --- docs/pipeline_flowchart.puml | 48 ++++++++++++++++++++++++++++++++++++ 1 file changed, 48 insertions(+) create mode 100644 docs/pipeline_flowchart.puml diff --git a/docs/pipeline_flowchart.puml b/docs/pipeline_flowchart.puml new file mode 100644 index 00000000..c810785a --- /dev/null +++ b/docs/pipeline_flowchart.puml @@ -0,0 +1,48 @@ + +!include pipeline_elements.iuml!string_functions +!include pipeline_elements.iuml!input_rect +!include pipeline_elements.iuml!output_rect +!include pipeline_elements.iuml!intermediate_rect +!include pipeline_elements.iuml!qc_rect +!include pipeline_elements.iuml!process_legend + +skinparam linetype ortho + +@startuml mutect2_chart + +$input_process(normal_bam, 'Normal bam', 'input normal bam file') +$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') +$input_process(contamination_table, 'Contamination Table: optional', 'output of GATK %newline()CalculateContamination') +$input_process(reference_genome, 'Reference genome', 'fasta file') +$input_process(germline_resource, 'Germline resource', 'gnomad VCF') + + +$intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome split into %newline()params.scatter_count intervals') +$intermediate_process(mutect2_call_sSNV, 'GATK Mutect2: one process per interval', 'Output: unfiltered VCFs, variant stats, F1R2 metrics') +$intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') +$intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats') +$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: orientation bias artifacts table') +$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF %newline()Optional output: filtering stats') +$output_process(awk_filterVCF, 'remove non-PASS variants', 'Output: filtered-pass.vcf.gz') +$qc_process(gatk_filteringStats, 'Optional', 'Output: filteringStats.tsv') + +reference_genome -d-> gatk_splitIntervals +normal_bam -d-> mutect2_call_sSNV +tumor_bam -d-> mutect2_call_sSNV +gatk_splitIntervals -d-> mutect2_call_sSNV +germline_resource -d-> mutect2_call_sSNV +mutect2_call_sSNV -d-> gatk_mergeVcfs +mutect2_call_sSNV -d-> gatk_learnReadOrientation +mutect2_call_sSNV -d-> mutect2_mergeStats + +gatk_mergeVcfs -d-> mutect2_filterCalls : // unfiltered.vcf.gz // +gatk_learnReadOrientation -d-> mutect2_filterCalls : // f1r2.tar.gz // +mutect2_mergeStats -d-> mutect2_filterCalls : // unfiltered.vcf.stats.gz // +reference_genome -d-> mutect2_filterCalls +contamination_table -d-> mutect2_filterCalls +mutect2_filterCalls -d-> gatk_filteringStats +mutect2_filterCalls -d-> awk_filterVCF : // filtered.vcf.gz // + +$add_legend('left') + +@enduml From 0487debec275ef10691cb5244026ca1e9f08b230 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 10:34:15 -0800 Subject: [PATCH 10/16] testing plantUML action: re-add muse as pipeline_flowchart.puml --- docs/pipeline_elements.puml | 27 +++++++++++++++++++++++++++ 1 file changed, 27 insertions(+) create mode 100644 docs/pipeline_elements.puml diff --git a/docs/pipeline_elements.puml b/docs/pipeline_elements.puml new file mode 100644 index 00000000..2254b189 --- /dev/null +++ b/docs/pipeline_elements.puml @@ -0,0 +1,27 @@ + +!include pipeline_elements.iuml!string_functions +!include pipeline_elements.iuml!input_rect +!include pipeline_elements.iuml!output_rect +!include pipeline_elements.iuml!intermediate_rect +!include pipeline_elements.iuml!qc_rect +!include pipeline_elements.iuml!process_legend + +skinparam linetype ortho + +@startuml muse_chart + +$input_process(normal_bam, 'Normal bam', 'input normal bam file') +$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') + +$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') +$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') +$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') + +tumor_bam -d-> muse_call_run +normal_bam -d-> muse_call_run +muse_call_run -d-> muse_sump_run +dbsnp_file -d-> muse_sump_run + +$add_legend('left') + +@enduml From 61143a2b7561837218b43a63ad89880fc1ab8e4c Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 10:41:08 -0800 Subject: [PATCH 11/16] testing plantUML action: concat .iuml and .puml --- docs/muse_flowchart.puml | 78 ++++++++++++++++++++++++++++++++++++++++ 1 file changed, 78 insertions(+) create mode 100644 docs/muse_flowchart.puml diff --git a/docs/muse_flowchart.puml b/docs/muse_flowchart.puml new file mode 100644 index 00000000..2d88b6fd --- /dev/null +++ b/docs/muse_flowchart.puml @@ -0,0 +1,78 @@ + +@startuml(id=string_functions) +!unquoted function $comma_to_newline($input_str="") +!local $arg_str = '' +!foreach $item in %splitstr($input_str, ",") + !local $arg_str = $arg_str + " %newline()" + $item +!endfor +!return %substr($arg_str, 11) +!endfunction +@enduml + +@startuml(id=input_rect) +!unquoted procedure $input_process($alias, $title, $description) +rectangle $alias #LightBlue as " +$title +==== +$description" +!endprocedure +@enduml + +@startuml(id=intermediate_rect) +!unquoted procedure $intermediate_process($alias, $title, $description) +rectangle $alias #White as " +$title +==== +$description" +!endprocedure +@enduml + +@startuml(id=output_rect) +!unquoted procedure $output_process($alias, $title, $description) +rectangle $alias #LightGreen as " +$title +==== +$description" +!endprocedure +@enduml + +@startuml(id=qc_rect) +!unquoted procedure $qc_process($alias, $title, $description) +rectangle $alias #Yellow as " +$title +==== +$description" +!endprocedure +@enduml + +@startuml(id=process_legend) +!unquoted procedure $add_legend($pos="bottom right") +legend $pos + |Color|Process Type| + |<#LightBlue>| Input | + |<#White>| Intermediate | + |<#LightGreen>| Output | + |<#Yellow>| QC | +endlegend +!endprocedure +@enduml + +skinparam linetype ortho + +@startuml muse_chart + +$input_process(normal_bam, 'Normal bam', 'input normal bam file') +$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') + +$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') +$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') +$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') + +tumor_bam -d-> muse_call_run +normal_bam -d-> muse_call_run +muse_call_run -d-> muse_sump_run +dbsnp_file -d-> muse_sump_run + +$add_legend('left') + +@enduml From 531562596e2753625f00d9eea3e0faf06f428ab7 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 15:06:27 -0800 Subject: [PATCH 12/16] single file mutect2 puml --- docs/mutect2_flowchart.puml | 99 +++++++++++++++++++++++++++++++++++++ 1 file changed, 99 insertions(+) create mode 100644 docs/mutect2_flowchart.puml diff --git a/docs/mutect2_flowchart.puml b/docs/mutect2_flowchart.puml new file mode 100644 index 00000000..c40a47d3 --- /dev/null +++ b/docs/mutect2_flowchart.puml @@ -0,0 +1,99 @@ +@startuml(id=string_functions) +!unquoted function $comma_to_newline($input_str="") +!local $arg_str = '' +!foreach $item in %splitstr($input_str, ",") + !local $arg_str = $arg_str + " %newline()" + $item +!endfor +!return %substr($arg_str, 11) +!endfunction +@enduml + +@startuml(id=input_rect) +!unquoted procedure $input_process($alias, $title, $description) +rectangle $alias #LightBlue as " +$title +==== +$description" +!endprocedure +@enduml + +@startuml(id=intermediate_rect) +!unquoted procedure $intermediate_process($alias, $title, $description) +rectangle $alias #White as " +$title +==== +$description" +!endprocedure +@enduml + +@startuml(id=output_rect) +!unquoted procedure $output_process($alias, $title, $description) +rectangle $alias #LightGreen as " +$title +==== +$description" +!endprocedure +@enduml + + +@startuml(id=qc_rect) +!unquoted procedure $qc_process($alias, $title, $description) +rectangle $alias #Yellow as " +$title +==== +$description" +!endprocedure +@enduml + +@startuml(id=process_legend) +!unquoted procedure $add_legend($pos="bottom right") +legend $pos + |Color|Process Type| + |<#LightBlue>| Input | + |<#White>| Intermediate | + |<#LightGreen>| Output | + |<#Yellow>| QC | +endlegend +!endprocedure +@enduml + +skinparam linetype ortho + +@startuml mutect2_chart + +$input_process(normal_bam, 'Normal bam', 'input normal bam file') +$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') +$input_process(contamination_table, 'Contamination Table: optional', 'output of GATK %newline()CalculateContamination') +$input_process(reference_genome, 'Reference genome', 'fasta file') +$input_process(germline_resource, 'Germline resource', 'gnomad VCF') + + +$intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome split into %newline()params.scatter_count intervals') +$intermediate_process(mutect2_call_sSNV, 'GATK Mutect2: one process per interval', 'Output: unfiltered VCFs, variant stats, F1R2 metrics') +$intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') +$intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats') +$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: orientation bias artifacts table') +$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF %newline()Optional output: filtering stats') +$output_process(awk_filterVCF, 'remove non-PASS variants', 'Output: filtered-pass.vcf.gz') +$qc_process(gatk_filteringStats, 'Optional', 'Output: filteringStats.tsv') + +reference_genome -d-> gatk_splitIntervals +normal_bam -d-> mutect2_call_sSNV +tumor_bam -d-> mutect2_call_sSNV +gatk_splitIntervals -d-> mutect2_call_sSNV +germline_resource -d-> mutect2_call_sSNV +mutect2_call_sSNV -d-> gatk_mergeVcfs +mutect2_call_sSNV -d-> gatk_learnReadOrientation +mutect2_call_sSNV -d-> mutect2_mergeStats + +gatk_mergeVcfs -d-> mutect2_filterCalls : // unfiltered.vcf.gz // +gatk_learnReadOrientation -d-> mutect2_filterCalls : // f1r2.tar.gz // +mutect2_mergeStats -d-> mutect2_filterCalls : // unfiltered.vcf.stats.gz // +reference_genome -d-> mutect2_filterCalls +contamination_table -d-> mutect2_filterCalls +mutect2_filterCalls -d-> gatk_filteringStats +mutect2_filterCalls -d-> awk_filterVCF : // filtered.vcf.gz // + +$add_legend('left') + +@enduml From 2d84acde32fe8d8c0d98c1dc360c3847b07f5459 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Fri, 10 Mar 2023 15:11:28 -0800 Subject: [PATCH 13/16] reconfigure per Aaron suggestions --- docs/muse_flowchart.puml | 78 ------------------------------------ docs/mutect2_flowchart.puml | 18 +-------- docs/pipeline_elements.iuml | 57 -------------------------- docs/pipeline_elements.puml | 27 ------------- docs/pipeline_flowchart.puml | 48 ---------------------- 5 files changed, 2 insertions(+), 226 deletions(-) delete mode 100644 docs/muse_flowchart.puml delete mode 100644 docs/pipeline_elements.iuml delete mode 100644 docs/pipeline_elements.puml delete mode 100644 docs/pipeline_flowchart.puml diff --git a/docs/muse_flowchart.puml b/docs/muse_flowchart.puml deleted file mode 100644 index 2d88b6fd..00000000 --- a/docs/muse_flowchart.puml +++ /dev/null @@ -1,78 +0,0 @@ - -@startuml(id=string_functions) -!unquoted function $comma_to_newline($input_str="") -!local $arg_str = '' -!foreach $item in %splitstr($input_str, ",") - !local $arg_str = $arg_str + " %newline()" + $item -!endfor -!return %substr($arg_str, 11) -!endfunction -@enduml - -@startuml(id=input_rect) -!unquoted procedure $input_process($alias, $title, $description) -rectangle $alias #LightBlue as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=intermediate_rect) -!unquoted procedure $intermediate_process($alias, $title, $description) -rectangle $alias #White as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=output_rect) -!unquoted procedure $output_process($alias, $title, $description) -rectangle $alias #LightGreen as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=qc_rect) -!unquoted procedure $qc_process($alias, $title, $description) -rectangle $alias #Yellow as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=process_legend) -!unquoted procedure $add_legend($pos="bottom right") -legend $pos - |Color|Process Type| - |<#LightBlue>| Input | - |<#White>| Intermediate | - |<#LightGreen>| Output | - |<#Yellow>| QC | -endlegend -!endprocedure -@enduml - -skinparam linetype ortho - -@startuml muse_chart - -$input_process(normal_bam, 'Normal bam', 'input normal bam file') -$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') - -$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') -$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') -$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') - -tumor_bam -d-> muse_call_run -normal_bam -d-> muse_call_run -muse_call_run -d-> muse_sump_run -dbsnp_file -d-> muse_sump_run - -$add_legend('left') - -@enduml diff --git a/docs/mutect2_flowchart.puml b/docs/mutect2_flowchart.puml index c40a47d3..e8e0bf47 100644 --- a/docs/mutect2_flowchart.puml +++ b/docs/mutect2_flowchart.puml @@ -1,4 +1,5 @@ -@startuml(id=string_functions) +@startuml mutect2_chart + !unquoted function $comma_to_newline($input_str="") !local $arg_str = '' !foreach $item in %splitstr($input_str, ",") @@ -6,46 +7,35 @@ !endfor !return %substr($arg_str, 11) !endfunction -@enduml -@startuml(id=input_rect) !unquoted procedure $input_process($alias, $title, $description) rectangle $alias #LightBlue as " $title ==== $description" !endprocedure -@enduml -@startuml(id=intermediate_rect) !unquoted procedure $intermediate_process($alias, $title, $description) rectangle $alias #White as " $title ==== $description" !endprocedure -@enduml -@startuml(id=output_rect) !unquoted procedure $output_process($alias, $title, $description) rectangle $alias #LightGreen as " $title ==== $description" !endprocedure -@enduml - -@startuml(id=qc_rect) !unquoted procedure $qc_process($alias, $title, $description) rectangle $alias #Yellow as " $title ==== $description" !endprocedure -@enduml -@startuml(id=process_legend) !unquoted procedure $add_legend($pos="bottom right") legend $pos |Color|Process Type| @@ -55,19 +45,15 @@ legend $pos |<#Yellow>| QC | endlegend !endprocedure -@enduml skinparam linetype ortho -@startuml mutect2_chart - $input_process(normal_bam, 'Normal bam', 'input normal bam file') $input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') $input_process(contamination_table, 'Contamination Table: optional', 'output of GATK %newline()CalculateContamination') $input_process(reference_genome, 'Reference genome', 'fasta file') $input_process(germline_resource, 'Germline resource', 'gnomad VCF') - $intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome split into %newline()params.scatter_count intervals') $intermediate_process(mutect2_call_sSNV, 'GATK Mutect2: one process per interval', 'Output: unfiltered VCFs, variant stats, F1R2 metrics') $intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') diff --git a/docs/pipeline_elements.iuml b/docs/pipeline_elements.iuml deleted file mode 100644 index 416b27b2..00000000 --- a/docs/pipeline_elements.iuml +++ /dev/null @@ -1,57 +0,0 @@ -@startuml(id=string_functions) -!unquoted function $comma_to_newline($input_str="") -!local $arg_str = '' -!foreach $item in %splitstr($input_str, ",") - !local $arg_str = $arg_str + " %newline()" + $item -!endfor -!return %substr($arg_str, 11) -!endfunction -@enduml - -@startuml(id=input_rect) -!unquoted procedure $input_process($alias, $title, $description) -rectangle $alias #LightBlue as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=intermediate_rect) -!unquoted procedure $intermediate_process($alias, $title, $description) -rectangle $alias #White as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=output_rect) -!unquoted procedure $output_process($alias, $title, $description) -rectangle $alias #LightGreen as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=qc_rect) -!unquoted procedure $qc_process($alias, $title, $description) -rectangle $alias #Yellow as " -$title -==== -$description" -!endprocedure -@enduml - -@startuml(id=process_legend) -!unquoted procedure $add_legend($pos="bottom right") -legend $pos - |Color|Process Type| - |<#LightBlue>| Input | - |<#White>| Intermediate | - |<#LightGreen>| Output | - |<#Yellow>| QC | -endlegend -!endprocedure -@enduml diff --git a/docs/pipeline_elements.puml b/docs/pipeline_elements.puml deleted file mode 100644 index 2254b189..00000000 --- a/docs/pipeline_elements.puml +++ /dev/null @@ -1,27 +0,0 @@ - -!include pipeline_elements.iuml!string_functions -!include pipeline_elements.iuml!input_rect -!include pipeline_elements.iuml!output_rect -!include pipeline_elements.iuml!intermediate_rect -!include pipeline_elements.iuml!qc_rect -!include pipeline_elements.iuml!process_legend - -skinparam linetype ortho - -@startuml muse_chart - -$input_process(normal_bam, 'Normal bam', 'input normal bam file') -$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') - -$intermediate_process(muse_call_run, 'MuSE call', 'Output: position-specific %newline()summary statistics') -$input_process(dbsnp_file, 'dbSNP', 'dbSNP VCF') -$output_process(muse_sump_run, 'MuSE sump', 'Output: tier-based %newline()cutoffs') - -tumor_bam -d-> muse_call_run -normal_bam -d-> muse_call_run -muse_call_run -d-> muse_sump_run -dbsnp_file -d-> muse_sump_run - -$add_legend('left') - -@enduml diff --git a/docs/pipeline_flowchart.puml b/docs/pipeline_flowchart.puml deleted file mode 100644 index c810785a..00000000 --- a/docs/pipeline_flowchart.puml +++ /dev/null @@ -1,48 +0,0 @@ - -!include pipeline_elements.iuml!string_functions -!include pipeline_elements.iuml!input_rect -!include pipeline_elements.iuml!output_rect -!include pipeline_elements.iuml!intermediate_rect -!include pipeline_elements.iuml!qc_rect -!include pipeline_elements.iuml!process_legend - -skinparam linetype ortho - -@startuml mutect2_chart - -$input_process(normal_bam, 'Normal bam', 'input normal bam file') -$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') -$input_process(contamination_table, 'Contamination Table: optional', 'output of GATK %newline()CalculateContamination') -$input_process(reference_genome, 'Reference genome', 'fasta file') -$input_process(germline_resource, 'Germline resource', 'gnomad VCF') - - -$intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome split into %newline()params.scatter_count intervals') -$intermediate_process(mutect2_call_sSNV, 'GATK Mutect2: one process per interval', 'Output: unfiltered VCFs, variant stats, F1R2 metrics') -$intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') -$intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats') -$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: orientation bias artifacts table') -$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF %newline()Optional output: filtering stats') -$output_process(awk_filterVCF, 'remove non-PASS variants', 'Output: filtered-pass.vcf.gz') -$qc_process(gatk_filteringStats, 'Optional', 'Output: filteringStats.tsv') - -reference_genome -d-> gatk_splitIntervals -normal_bam -d-> mutect2_call_sSNV -tumor_bam -d-> mutect2_call_sSNV -gatk_splitIntervals -d-> mutect2_call_sSNV -germline_resource -d-> mutect2_call_sSNV -mutect2_call_sSNV -d-> gatk_mergeVcfs -mutect2_call_sSNV -d-> gatk_learnReadOrientation -mutect2_call_sSNV -d-> mutect2_mergeStats - -gatk_mergeVcfs -d-> mutect2_filterCalls : // unfiltered.vcf.gz // -gatk_learnReadOrientation -d-> mutect2_filterCalls : // f1r2.tar.gz // -mutect2_mergeStats -d-> mutect2_filterCalls : // unfiltered.vcf.stats.gz // -reference_genome -d-> mutect2_filterCalls -contamination_table -d-> mutect2_filterCalls -mutect2_filterCalls -d-> gatk_filteringStats -mutect2_filterCalls -d-> awk_filterVCF : // filtered.vcf.gz // - -$add_legend('left') - -@enduml From 7552b1292ccd7e9558f389e9f8af94eeb5adcb13 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Mon, 20 Mar 2023 14:54:05 -0700 Subject: [PATCH 14/16] update and tmp manual placement of mutect2 flowchart --- README.md | 4 +- docs/muse_flowchart.puml | 28 ++++++++++++++ docs/mutect2_flowchart.puml | 71 ++++++++++-------------------------- image/mutect2.png | Bin 0 -> 291938 bytes 4 files changed, 49 insertions(+), 54 deletions(-) create mode 100644 docs/muse_flowchart.puml create mode 100644 image/mutect2.png diff --git a/README.md b/README.md index e32c64c2..b28a9d83 100644 --- a/README.md +++ b/README.md @@ -93,7 +93,7 @@ Version: v2.9.10 (Released on Nov 7, 2018) GitHub Package: ghcr.io/uclahs-cds/strelka2:2.9.10 ### Mutect 2 -![alt text](docs/mutect2_chart.svg?raw=true) +![Diagram](image/mutect2.png) #### Tools ##### GATK GATK source: https://github.com/broadinstitute/gatk @@ -243,7 +243,7 @@ input: | gatk_command_mem_diff | yes | nextflow.util.MemoryUnit | How much to subtract from the task's allocated memory where the remainder is the Java heap max. (should not be changed unless task fails for memory related reasons) | | scatter_count | yes | int | Number of intervals to split the desired interval into. Mutect2 will call each interval seperately. | | intervals | no | string | A GATK accepted interval list file containing intervals to search for somatic mutations.
If empty or missing, will optimally partition canonical genome based on scatter_count and process non-canonical regions separately. This is the default use case.
If specified and evaluates to a valid path, will pass that path to GATK to restrict the genomic regions searched. | -| germline_resource_gnomad_vcf | no | path | A copy of the gnomAD VCF only kept AF but stripped of all unnecessary INFO fields, currently available for GRCh38:`/hot/ref/tool-specific-input/GATK/GRCh38/af-only-gnomad.hg38.vcf.gz` and GRCh37: `/hot/ref/tool-specific-input/GATK/GRCh37/af-only-gnomad.raw.sites.vcf`. | +| germline_resource_gnomad_vcf | no | path | A stripped down version of the [gnomAD VCF](https://gnomad.broadinstitute.org/) stripped of all unneeded INFO fields, keeping only AF, currently available for GRCh38:`/hot/ref/tool-specific-input/GATK/GRCh38/af-only-gnomad.hg38.vcf.gz` and GRCh37: `/hot/ref/tool-specific-input/GATK/GRCh37/af-only-gnomad.raw.sites.vcf`. | #### MuSE Specific Configuration diff --git a/docs/muse_flowchart.puml b/docs/muse_flowchart.puml new file mode 100644 index 00000000..4fd3e599 --- /dev/null +++ b/docs/muse_flowchart.puml @@ -0,0 +1,28 @@ +@startuml muse_chart + +!include pipeline_elements.iuml!string_functions +!include pipeline_elements.iuml!input_rect +!include pipeline_elements.iuml!output_rect +!include pipeline_elements.iuml!intermediate_rect +!include pipeline_elements.iuml!qc_rect +!include pipeline_elements.iuml!process_legend + +skinparam linetype ortho + +$input_process(normal_bam, 'Normal BAM', 'Input: normal BAM file') +$input_process(tumor_bam, 'Tumor BAM', 'Input: tumor BAM file') + +$intermediate_process(muse_call_run, "MuSE call", "Output: position-specific %newline()summary statistics") +$input_process(dbsnp_file, 'dbSNP', 'Input: dbSNP VCF') +$intermediate_process(muse_sump_run, "MuSE sump", "Output: VCF with tier-based %newline()quality cutoffs") +$output_process(filter_vcf, 'Filter VCF', 'Output: VCF with top tier %newline()somatic variants') + +tumor_bam -d-> muse_call_run +normal_bam -d-> muse_call_run +muse_call_run -d-> muse_sump_run +dbsnp_file -d-> muse_sump_run +muse_sump_run -d-> filter_vcf + +$add_legend("left") + +@enduml diff --git a/docs/mutect2_flowchart.puml b/docs/mutect2_flowchart.puml index e8e0bf47..826cbeab 100644 --- a/docs/mutect2_flowchart.puml +++ b/docs/mutect2_flowchart.puml @@ -1,65 +1,26 @@ @startuml mutect2_chart -!unquoted function $comma_to_newline($input_str="") -!local $arg_str = '' -!foreach $item in %splitstr($input_str, ",") - !local $arg_str = $arg_str + " %newline()" + $item -!endfor -!return %substr($arg_str, 11) -!endfunction - -!unquoted procedure $input_process($alias, $title, $description) -rectangle $alias #LightBlue as " -$title -==== -$description" -!endprocedure - -!unquoted procedure $intermediate_process($alias, $title, $description) -rectangle $alias #White as " -$title -==== -$description" -!endprocedure - -!unquoted procedure $output_process($alias, $title, $description) -rectangle $alias #LightGreen as " -$title -==== -$description" -!endprocedure - -!unquoted procedure $qc_process($alias, $title, $description) -rectangle $alias #Yellow as " -$title -==== -$description" -!endprocedure - -!unquoted procedure $add_legend($pos="bottom right") -legend $pos - |Color|Process Type| - |<#LightBlue>| Input | - |<#White>| Intermediate | - |<#LightGreen>| Output | - |<#Yellow>| QC | -endlegend -!endprocedure +!include pipeline_elements.iuml!string_functions +!include pipeline_elements.iuml!input_rect +!include pipeline_elements.iuml!output_rect +!include pipeline_elements.iuml!intermediate_rect +!include pipeline_elements.iuml!qc_rect +!include pipeline_elements.iuml!process_legend skinparam linetype ortho -$input_process(normal_bam, 'Normal bam', 'input normal bam file') -$input_process(tumor_bam, 'Tumor bam', 'input tumor bam file') -$input_process(contamination_table, 'Contamination Table: optional', 'output of GATK %newline()CalculateContamination') -$input_process(reference_genome, 'Reference genome', 'fasta file') -$input_process(germline_resource, 'Germline resource', 'gnomad VCF') +$input_process(normal_bam, 'Normal BAM', 'Input: normal BAM file') +$input_process(tumor_bam, 'Tumor BAM', 'Input: tumor BAM file') +$input_process(contamination_table, 'Contamination Table: optional', 'Input: output of GATK %newline()CalculateContamination') +$input_process(reference_genome, 'Reference genome', 'Input: fasta file') +$input_process(germline_resource, 'Germline resource', 'Input: stripped gnomad VCF') $intermediate_process(gatk_splitIntervals, 'GATK SplitIntervals', 'Output: reference genome split into %newline()params.scatter_count intervals') $intermediate_process(mutect2_call_sSNV, 'GATK Mutect2: one process per interval', 'Output: unfiltered VCFs, variant stats, F1R2 metrics') $intermediate_process(gatk_mergeVcfs, 'GATK MergeVcfs', 'Output: unfiltered VCF') $intermediate_process(mutect2_mergeStats, 'GATK MergeMutectStats', 'Output: Mutect2 stats') -$intermediate_process(gatk_learnReadOrientation, 'gatk LearnReadOrientationModel', 'Output: orientation bias artifacts table') -$intermediate_process(mutect2_filterCalls, 'gatk FilterMutectCalls', 'Output: gatk filtered VCF %newline()Optional output: filtering stats') +$intermediate_process(gatk_learnReadOrientation, 'GATK LearnReadOrientationModel', 'Output: orientation bias artifacts table') +$intermediate_process(mutect2_filterCalls, 'GATK FilterMutectCalls', 'Output: GATK filtered VCF %newline()Optional output: filtering stats') $output_process(awk_filterVCF, 'remove non-PASS variants', 'Output: filtered-pass.vcf.gz') $qc_process(gatk_filteringStats, 'Optional', 'Output: filteringStats.tsv') @@ -71,6 +32,12 @@ germline_resource -d-> mutect2_call_sSNV mutect2_call_sSNV -d-> gatk_mergeVcfs mutect2_call_sSNV -d-> gatk_learnReadOrientation mutect2_call_sSNV -d-> mutect2_mergeStats +mutect2_call_sSNV -d-> gatk_mergeVcfs +mutect2_call_sSNV -d-> gatk_mergeVcfs +mutect2_call_sSNV -d-> gatk_learnReadOrientation +mutect2_call_sSNV -d-> mutect2_mergeStats +mutect2_call_sSNV -d-> gatk_learnReadOrientation +mutect2_call_sSNV -d-> mutect2_mergeStats gatk_mergeVcfs -d-> mutect2_filterCalls : // unfiltered.vcf.gz // gatk_learnReadOrientation -d-> mutect2_filterCalls : // f1r2.tar.gz // diff --git a/image/mutect2.png b/image/mutect2.png new file mode 100644 index 0000000000000000000000000000000000000000..d16295620f951ff97a8b87f1973f8e6bd5f5b27f GIT binary patch literal 291938 zcmeFZby!u~76*!mA|PE75=tnIAYFopG}4WfgmgEeq)142NOyNBAYIbEX(Tt@?EThO z@o>)Jo_pW>-XAZHZax-ktvTnIV~#oEH^$t4GEyR_$b`snaB!%i&!5V|!QG03gS#1g z>n3oA!f03&4i354NJvOVR7i+S#>)Jak%>MW+;hM1NJM!#73?=)XU4idtb1gXdsNS< z--PuEebf{tr?@8QM~)%C-Z3XT{Pz7VMH}3wUlbk|`}*EM@qc-J?JeEC)oSHJ?PF4l zTbqz&D0El4hj+Km(b`~X+Sj2gg#-W^T7b6p~nOMsF{U_B=IxNqAf3}TpNXWWfZTy5ty~2Y=@KCli_CZiTu!# z?C{Bid=5qUK}zpu%KN&^r24FkOoFb>52Tw7DBbX>kIk8}^_iS8NbinCA-}Q7b?AkP9BBU1(c+mEn2XG29cMrA4OG z+uZd&VyFe*!KwS`Y1`S?vCU%jxxJUs4P;XyuX;O>Lc6=4p)Yhzz8oV-w>p2DLEr$- zi17vzMRrH|8qrH6lP9;}#@?o38{jz5;W38Z3Y9C4Hrb0niXz7BbwL~GXzU<-A?!F{Wq*eC>_=_0Nuk(lqVS#ggjOZ*EYK 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new file mode 100644 index 00000000..c9046877 --- /dev/null +++ b/docs/muse_chart.svg @@ -0,0 +1,99 @@ +Normal BAMInput: normal BAM fileTumor BAMInput: tumor BAM fileMuSE callOutput: position-specificsummary statisticsdbSNPInput: dbSNP VCFMuSE sumpOutput: VCF with tier-basedquality cutoffsFilter VCFOutput: VCF with top tiersomatic variantsColorProcess Type Input Intermediate Output QC \ No newline at end of file diff --git a/docs/mutect2_chart.svg b/docs/mutect2_chart.svg new file mode 100644 index 00000000..a925ac84 --- /dev/null +++ b/docs/mutect2_chart.svg @@ -0,0 +1,191 @@ +Normal BAMInput: normal BAM fileTumor BAMInput: tumor BAM fileContamination Table: optionalInput: output of GATKCalculateContaminationReference genomeInput: fasta fileGermline resourceInput: stripped gnomad VCFGATK SplitIntervalsOutput: reference genome split intoparams.scatter_count intervalsGATK Mutect2: one process per intervalOutput: unfiltered VCFs, variant stats, F1R2 metricsGATK MergeVcfsOutput: unfiltered VCFGATK MergeMutectStatsOutput: Mutect2 statsGATK LearnReadOrientationModelOutput: orientation bias artifacts tableGATK FilterMutectCallsOutput: GATK filtered VCFOptional output: filtering statsremove non-PASS variantsOutput: filtered-pass.vcf.gzOptionalOutput: filteringStats.tsvunfiltered.vcf.gzf1r2.tar.gzunfiltered.vcf.stats.gzfiltered.vcf.gzColorProcess Type Input Intermediate Output QC \ No newline at end of file From 3e5a4e11666f683e190cf89389827340754c7736 Mon Sep 17 00:00:00 2001 From: Sorel Fitz-Gibbon Date: Mon, 20 Mar 2023 15:02:11 -0700 Subject: [PATCH 16/16] revert tmp fix --- README.md | 2 +- image/mutect2.png | Bin 291938 -> 0 bytes 2 files changed, 1 insertion(+), 1 deletion(-) delete mode 100644 image/mutect2.png diff --git a/README.md b/README.md index b28a9d83..ab00f37c 100644 --- a/README.md +++ b/README.md @@ -93,7 +93,7 @@ Version: v2.9.10 (Released on Nov 7, 2018) GitHub Package: ghcr.io/uclahs-cds/strelka2:2.9.10 ### Mutect 2 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