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I am using semla and stutility to run spatial transcriptomic data. following their vignette when calculating nearest cluster neighbors to certain clusters I input a column thats nearest to cluster 4 for example, other spots that are not close to cluster 4 will be NA but when I run and try to map it - dplyr doesnt seem to be able to combine columns because the rows dont match. which is odd.
> EWS4064$cluster_4 <- ifelse(EWS4064$seurat_clusters %in% "4", "4", NA)
> MapLabels(EWS4064, column_name = "cluster_4", override_plot_dims = TRUE,
+ image_use = "raw", drop_na = TRUE, pt_size = 2) +
+ plot_layout(guides = "collect") &
+ theme(legend.position = "right") &
+ guides(fill = guide_legend(override.aes = list(size = 3), ncol = 2))
Error in `bind_cols()`:
! Can't recycle `..1` (size 1681) to match `..2` (size 74).
Run `rlang::last_trace()` to see where the error occurred.
Warning message:
Removing 1607 cells missing data for vars requested
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