Comparing scVelo and CytoTrace results

[gundalav]

I have the following scRNaseq analysis done with scVelo and CytoTrace.

My understanding is as follows:

1. scVelo arrows lead from less differentiated cell X (more stem cell) to more differentiated cell Y (e.g. granulocyte)
2. CytoTrace brighter color is more differentiated (e.g. granulocyte) to darker color less differentiated (e.g. more esteem)

If so, the two results I have above are totally opposite.
Is my interpretation correct? If so, why did it give the opposite result?
Which one should we trust: scVelo or CytoTrace?

[WeilerP]

@gundalav, given the limited info, I can only guess to why the results differ. If their is a problem with the inference in scVelo it is most likely due to uninformative phase portraits (see e.g. #216 or #462). CytoTRACE will only work if the base assumption is met, i.e. if less mature cells express more genes.
Concluding, you cannot make a general statement about trusting one method more than another, IMHO. It always depends on the dataset you are using whether or not the method may work.