From a1b29be3df26e31c73dfc9bede551af17966ba2d Mon Sep 17 00:00:00 2001 From: "github-actions[bot]" <41898282+github-actions[bot]@users.noreply.github.com> Date: Mon, 18 Dec 2023 05:22:34 +0000 Subject: [PATCH] Add changes --- _data/containers.yaml | 2654 +-- _data/repos.yml | 2753 +-- assets/js/repos.js | 35928 ++++++++++++++++++++-------------------- 3 files changed, 20665 insertions(+), 20670 deletions(-) diff --git a/_data/containers.yaml b/_data/containers.yaml index 2eb6f99b..5fe5ea68 100644 --- a/_data/containers.yaml +++ b/_data/containers.yaml @@ -62,265 +62,267 @@ bases: - count: 25 name: osrf/ros - count: 22 - name: khanlab/tar2bids + name: pennbbl/qsiprep - count: 22 name: nipreps/fmriprep -- count: 22 - name: pennbbl/qsiprep - count: 22 name: r-base - count: 22 name: continuumio/anaconda3 -- count: 18 - name: glotzerlab/software +- count: 22 + name: khanlab/tar2bids - count: 18 name: bensonyang88/tensorflow-rdma - count: 18 - name: nvcr.io/nvidia/nvhpc + name: glotzerlab/software - count: 18 - name: rocker/r-ver + name: nvcr.io/nvidia/nvhpc - count: 18 name: ezlabgva/busco +- count: 18 + name: rocker/r-ver - count: 16 name: rocker/tidyverse - count: 15 name: jtchilders/singularity_image_recipes -- count: 15 - name: khanlab/neuroglia-dwi - count: 15 name: ResearchIT/spack-singularity - count: 15 name: poldracklab/mriqc +- count: 15 + name: khanlab/neuroglia-dwi +- count: 14 + name: ebothmann/sherpa-singularity - count: 14 name: nipy/heudiconv - count: 14 name: mambaorg/micromamba -- count: 14 - name: ebothmann/sherpa-singularity - count: 14 name: shahzebmsiddiqui/default/easybuild - count: 13 name: nitishnarula/secure/ubuntu-focal -- count: 12 - name: khanlab/heudiconv -- count: 12 - name: conda/miniconda2 - count: 12 name: pawsey/openfoam - count: 12 - name: rocker/verse + name: openfluid/openfluid - count: 12 name: julia - count: 12 - name: openfluid/openfluid -- count: 12 - name: ufscar/ubuntu_ompi + name: conda/miniconda2 - count: 12 name: vitchyr/railrl-torch4cuda9 +- count: 12 + name: rocker/verse +- count: 12 + name: ufscar/ubuntu_ompi - count: 12 name: 'GodloveD/busybox ' +- count: 12 + name: khanlab/heudiconv - count: 11 - name: deeplearnphysics/larcv2 -- count: 11 - name: qiime2/core + name: sequana.img - count: 11 name: khanlab/hippunfold -- count: 11 - name: sequana.img - count: 11 name: hariszaf/pema - count: 11 - name: trinityrnaseq/trinityrnaseq + name: deeplearnphysics/larcv2 - count: 11 - name: golang + name: qiime2/core +- count: 11 + name: trinityrnaseq/trinityrnaseq - count: 11 name: rockylinux +- count: 11 + name: golang - count: 11 name: perl - count: 10 - name: khanlab/prepdwi + name: images/common.simg +- count: 10 + name: provarepro/openvino - count: 10 name: quay.io/vgteam/vg - count: 10 - name: images/common.simg + name: scientificlinux/sl - count: 10 name: intel/oneapi-hpckit - count: 10 - name: scientificlinux/sl -- count: 10 - name: provarepro/openvino -- count: 9 - name: DeepLearnPhysics/larcv3-singularity + name: khanlab/prepdwi - count: 9 name: nipreps/mriqc - count: 9 name: dcgc-bfx/dcgc-jupyter-rstudio - 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name: GodloveD -- count: 1 - name: nextgenusfs + name: bilbydev - count: 1 - name: combinelab + name: meren - count: 1 name: gnuoctave - count: 1 name: ezlabgva +- count: 1 + name: combinelab +- count: 1 + name: nextgenusfs - count: 1 name: pandoc - count: 1 - name: meren + name: chambm - count: 1 - name: condaforge + name: singularityhub - count: 1 - name: thakk + name: mvdenbog - count: 1 - name: archlinux + name: eddelbuettel - count: 1 - name: mustxyk + name: ucrdocker - count: 1 - name: penngwyn + name: ucr-singularity - count: 1 - name: jtduda + name: bvlc +- count: 1 + name: nitesh1989 tags: - latest: 686 + latest: 683 other: 5824 versions: /nrs/funke/singularity/linajea/pylp_base: @@ -4107,7 +4107,7 @@ versions: larbys/uboonecode: v08_00_00_40: 1 letaylor/sc_qc_cluster: - latest: 5 + latest: 2 libatomsquip/quip-base: latest: 1 library/centos: diff --git a/_data/repos.yml b/_data/repos.yml index fc644220..43787b01 100644 --- a/_data/repos.yml +++ b/_data/repos.yml @@ -4468,26 +4468,26 @@ Asap7772/rail-rl-franka-eval: description: null filenames: - docker/Singularity - - docker/railrl_v11_cuda10-1_mj2-0-2-2_torch0-3-1_gym0-10-5_py3-5-2/Singularity - - docker/railrl_v7_cuda8/Singularity - - docker/railrl_v7/Singularity - - docker/railrl_gpu_mujoco1-5-v4/singularity/Singularity - - docker/railrl_v12_cuda10-1_mj2-0-2-2_torch1-1-0_gym0-12-5_py3-6-5/Singularity + - docker/railrl_v5/singularity/Singularity - docker/railrl_v12_cuda10-1_mj2-0-2-2_torch1-1-0_gym0-12-5_py3-6-5/Singularity_cpu + - docker/railrl_v12_cuda10-1_mj2-0-2-2_torch1-1-0_gym0-12-5_py3-6-5/Singularity + - docker/railrl_v6_cuda8/Singularity + - docker/railrl_v10_cuda10-1_mj2-0-2-2_torch0-4-1_gym0-10-5_py3-5-2/Singularity + - docker/railrl_v6_cuda9/Singularity + - docker/railrl_v7/Singularity - docker/railrl_v9_cuda10-1_mj1-50-1-59_torch0-4-1_gym0-10-5_py3-5-2/Singularity - - docker/railrl_v9-5_cuda10-1_mj1-50-1-59_torch1-1-0_gym0-10-5_py3-5-2/Singularity + - docker/railrl_v11_cuda10-1_mj2-0-2-2_torch0-3-1_gym0-10-5_py3-5-2/Singularity - docker/railrl_v8_cuda10-1/Singularity - - docker/railrl_v5/singularity/Singularity - - docker/railrl_v10_cuda10-1_mj2-0-2-2_torch0-4-1_gym0-10-5_py3-5-2/Singularity - - docker/railrl_ray_gym-0-12-0/Singularity_from_scratch_cuda8 - - docker/railrl_ray_gym-0-12-0/Singularity_from_scratch - - docker/railrl_hand_v2/Singularity - - docker/railrl_hand_v2/Singularity_cpu - docker/railrl_ray/Singularity - - docker/railrl_hand_v1/Singularity + - docker/railrl_hand_v2/Singularity_cpu + - docker/railrl_hand_v2/Singularity + - docker/railrl_v7_cuda8/Singularity + - docker/railrl_gpu_mujoco1-5-v4/singularity/Singularity + - docker/railrl_ray_gym-0-12-0/Singularity_from_scratch + - docker/railrl_ray_gym-0-12-0/Singularity_from_scratch_cuda8 + - docker/railrl_v9-5_cuda10-1_mj1-50-1-59_torch1-1-0_gym0-10-5_py3-5-2/Singularity - docker/railrl_hand_v1/Singularity_cpu - - docker/railrl_v6_cuda8/Singularity - - docker/railrl_v6_cuda9/Singularity + - docker/railrl_hand_v1/Singularity - experiments/ashvin/icml2020/singularity/Singularity full_name: Asap7772/rail-rl-franka-eval latest_release: null @@ -9535,10 +9535,10 @@ Bioconductor/bioconductor_full: description: DEPRECATED - Docker Images which include a complete installation of all software needed to build all Bioconductor packages filenames: - - Singularity + - Singularity.RELEASE_3_9 - Singularity.RELEASE_3_8 - Singularity.RELEASE_3_10 - - Singularity.RELEASE_3_9 + - Singularity full_name: Bioconductor/bioconductor_full latest_release: null readme: "

\n
wget https://swift.rc.nectar.org.au/v1/AUTH_810/CVL-Singularity-External-Files/virtualgl_2.6.2_amd64.deb\n\
     \ndpkg -i virtualgl_2.6.2_amd64.deb\n
\n" stargazers_count: 8 - subscribers_count: 5 + subscribers_count: 4 topics: [] updated_at: 1696247668.0 ChunCun/container: @@ -14292,8 +14292,8 @@ Crown421/Singularity.jl: data_format: 2 description: null filenames: - - basebuilds/Singularity.jupyterbase - basebuilds/Singularity.juliabase + - basebuilds/Singularity.jupyterbase full_name: Crown421/Singularity.jl latest_release: null readme: "

\n" - stargazers_count: 11 + stargazers_count: 13 subscribers_count: 2 topics: - collision-avoidance - gazebo-simulator - robot-navigation - updated_at: 1701582273.0 + updated_at: 1702557024.0 TheJacksonLaboratory/acm-bcb-singularity2021: data_format: 2 description: Singularity Course Repo for 2021 ACM-BCB Conference @@ -57730,15 +57730,15 @@ TheNoyesLab/WGS_SNP_pipelines: data_format: 2 description: Collection of SNP pipelines to analyze assembled genomes filenames: - - containers/Singularity - - containers/Singularity.etoki - - containers/Singularity.atlas - - containers/Singularity.lyveset1 - containers/Singularity.cfsansnp - containers/Singularity.tree_analysis + - containers/Singularity.peppa - containers/Singularity.lyveset - containers/Singularity.kSNP3_cfsansnp - - containers/Singularity.peppa + - containers/Singularity + - containers/Singularity.lyveset1 + - containers/Singularity.etoki + - containers/Singularity.atlas full_name: TheNoyesLab/WGS_SNP_pipelines latest_release: null readme: '

' - stargazers_count: 8334 + stargazers_count: 8341 subscribers_count: 349 topics: - c-plus-plus @@ -62171,7 +62171,7 @@ VowpalWabbit/vowpal_wabbit: - active-learning - learning-to-search - cpp - updated_at: 1702263131.0 + updated_at: 1702776911.0 WIPACrepo/monitoring-scripts: data_format: 2 description: Tools for monitoring HTCondor and other things @@ -64092,8 +64092,8 @@ aardk/jupyter-casa: data_format: 2 description: A Jupyter kernel for CASA filenames: - - singularity/Singularity - singularity/Singularity.docker + - singularity/Singularity - singularity/Singularity.centos7 full_name: aardk/jupyter-casa latest_release: null @@ -64962,8 +64962,8 @@ accre/singularity: description: Examples that use singularity containers to run arbitrary operating systems on the ACCRE cluster. filenames: - - accre-internal/Singularity.accre.internal.base - accre-internal/Singularity.accre.internal.rstudio.3.6.0 + - accre-internal/Singularity.accre.internal.base - accre-internal/Singularity.accre.internal.rstudio.4.0.5 - accre-internal/Singularity.accre.internal.rstudio.gpu.3.6.0 - accre-internal/Singularity.accre.internal.rstudio.3.4.3 @@ -69757,29 +69757,29 @@ apptainer/singularity: project to the Linux Foundation. This repo has been persisted as a snapshot right before the changes. filenames: + - e2e/testdata/Singularity - examples/instances/Singularity - - examples/opensuse/Singularity - - examples/apps/Singularity - - examples/apps/Singularity.cowsay - examples/debian/Singularity - - examples/arch/Singularity - - examples/scientific/Singularity - examples/shub/Singularity - - examples/multistage/Singularity - - examples/asciinema/Singularity + - examples/docker/Singularity + - examples/ubuntu/Singularity + - examples/raspbian/Singularity + - examples/apps/Singularity.cowsay + - examples/apps/Singularity + - examples/opensuse/Singularity + - examples/busybox/Singularity + - examples/sle/Singularity + - examples/arch/Singularity - examples/centos/Singularity - examples/self/Singularity + - examples/multistage/Singularity + - examples/scratch/Singularity.alpine + - examples/scratch/Singularity.busybox - examples/centos-arm64/Singularity - - examples/sle/Singularity - - examples/raspbian/Singularity - examples/library/Singularity - examples/opensuse-arm64/Singularity - - examples/scratch/Singularity.busybox - - examples/scratch/Singularity.alpine - - examples/docker/Singularity - - examples/busybox/Singularity - - examples/ubuntu/Singularity - - e2e/testdata/Singularity + - examples/scientific/Singularity + - examples/asciinema/Singularity full_name: apptainer/singularity latest_release: v3.8.7 readme: '

.

' - stargazers_count: 2474 + stargazers_count: 2476 subscribers_count: 91 topics: - containers @@ -70002,7 +70002,7 @@ apptainer/singularity: - portability - rootless-containers - cloud-native - updated_at: 1701850261.0 + updated_at: 1702753123.0 ar90n/lab: data_format: 2 description: my laboratory @@ -71574,21 +71574,21 @@ automl/HPOBench: description: Collection of hyperparameter optimization benchmark problems filenames: - hpobench/container/recipes/Singularity.template - - hpobench/container/recipes/ml/Singularity.XGBoostBenchmark + - hpobench/container/recipes/od/Singularity.ODBenchmarks + - hpobench/container/recipes/od/Singularity.ODKernelDensityEstimation + - hpobench/container/recipes/ml/Singularity.ml_mmfb - hpobench/container/recipes/ml/Singularity.SupportVectorMachine + - hpobench/container/recipes/ml/Singularity.XGBoostBenchmark - hpobench/container/recipes/ml/Singularity.PyBNN - hpobench/container/recipes/ml/Singularity.ml_tabular_benchmark - - hpobench/container/recipes/ml/Singularity.ml_mmfb - - hpobench/container/recipes/od/Singularity.ODKernelDensityEstimation - - hpobench/container/recipes/od/Singularity.ODBenchmarks - - hpobench/container/recipes/surrogates/Singularity.SupportVectorMachine - - hpobench/container/recipes/surrogates/Singularity.ParamnetBenchmark - - hpobench/container/recipes/rl/Singularity.Cartpole - - hpobench/container/recipes/rl/Singularity.learnaBenchmark - hpobench/container/recipes/nas/Singularity.nasbench_1shot1 - - hpobench/container/recipes/nas/Singularity.TabularBenchmarks - - hpobench/container/recipes/nas/Singularity.nasbench_201 - hpobench/container/recipes/nas/Singularity.nasbench_101 + - hpobench/container/recipes/nas/Singularity.nasbench_201 + - hpobench/container/recipes/nas/Singularity.TabularBenchmarks + - hpobench/container/recipes/rl/Singularity.Cartpole + - hpobench/container/recipes/rl/Singularity.learnaBenchmark + - hpobench/container/recipes/surrogates/Singularity.SupportVectorMachine + - hpobench/container/recipes/surrogates/Singularity.ParamnetBenchmark full_name: automl/HPOBench latest_release: 0.0.10 readme: "

Description

\n\ -

This BIDS App enables generation and subsequent group analysis of structural\ - \ connectomes\ngenerated from diffusion MRI data. The analysis pipeline relies\ - \ primarily on the MRtrix3\nsoftware package, and includes a number of\ - \ state-of-the-art methods for image processing,\ntractography reconstruction,\ - \ connectome generation and inter-subject connection density\nnormalisation.

\n\ -

NOTE: App is still under development; script is not guaranteed\ - \ to be operational\nfor all use cases.

\n

Requirements

\n

Due to use of the Anatomically-Constrained\ - \ Tractography (ACT) framework, correction of\nEPI susceptibility distortions\ - \ is a prerequisite for this pipeline. Currently, this is\nonly possible within\ - \ this pipeline through use of the FSL tool topup, which relies\n\ - on the presence of spin-echo EPI images with differences in phase encoding to\ - \ estimate\nthe causative inhomogeneity field. In the absence of such data, this\ - \ pipeline is not\ncurrently applicable; though recommendations for alternative\ - \ mechanisms for such\ncorrection in the Issues page are welcome, and development\ - \ of novel techniques for\nperforming this correction are additionally underway.

\n\ -

While many common DICOM conversion software are capable of providing data characterising\n\ - the phase and slice encoding performed in the acquisition protocol, which are\ - \ subsequently\nused by this pipeline to automate DWI data pre-processing, for\ - \ some softwares and/or\nsome data (particularly those not acquired on a Siemens\ - \ platform), such data may not be\npresent in the sidecar JSON files for files\ - \ in the BIDS dwi/ and fmap/ directories.\nIn this circumstance,\ - \ it will be necessary for users to manually enter the relevant\ninformation into\ - \ these files in order for this script to be capable of processing the\ndata.\ - \ Every JSON file in these two directories should contain the BIDS fields\nPhaseEncodingDirection\ + readme: "

Description

\n

This BIDS App enables generation\ + \ and subsequent group analysis of structural connectomes\ngenerated from diffusion\ + \ MRI data. The analysis pipeline relies primarily on the MRtrix3\nsoftware\ + \ package, and includes a number of state-of-the-art methods for image processing,\n\ + tractography reconstruction, connectome generation and inter-subject connection\ + \ density\nnormalisation.

\n

NOTE: App is still under development;\ + \ script is not guaranteed to be operational\nfor all use cases.

\n

Requirements

\n

Due to use of the Anatomically-Constrained Tractography\ + \ (ACT) framework, correction of\nEPI susceptibility distortions is a prerequisite\ + \ for this pipeline. Currently, this is\nonly possible within this pipeline through\ + \ use of the FSL tool topup, which relies\non the presence of spin-echo\ + \ EPI images with differences in phase encoding to estimate\nthe causative inhomogeneity\ + \ field. In the absence of such data, this pipeline is not\ncurrently applicable;\ + \ though recommendations for alternative mechanisms for such\ncorrection in the\ + \ Issues page are welcome, and development of novel techniques for\nperforming\ + \ this correction are additionally underway.

\n

While many common DICOM conversion\ + \ software are capable of providing data characterising\nthe phase and slice encoding\ + \ performed in the acquisition protocol, which are subsequently\nused by this\ + \ pipeline to automate DWI data pre-processing, for some softwares and/or\nsome\ + \ data (particularly those not acquired on a Siemens platform), such data may\ + \ not be\npresent in the sidecar JSON files for files in the BIDS dwi/\ + \ and fmap/ directories.\nIn this circumstance, it will be necessary\ + \ for users to manually enter the relevant\ninformation into these files in order\ + \ for this script to be capable of processing the\ndata. Every JSON file in these\ + \ two directories should contain the BIDS fields\nPhaseEncodingDirection\ \ and TotalReadoutTime. For DWI data, it is also preferable to\n\ provide the SliceEncodingDirection and SliceTiming fields.\ \ More information on these\ndata can be found in the BIDS documentation.

\n

Instructions

\n

Multi-stage\ - \ analysis pipeline

\n\ + \ rel=\"nofollow\">BIDS documentation.

\n

Instructions

\n\ +

Multi-stage analysis pipeline

\n\

The tool consists of three explicit analysis levels:

\n
    \n
  1. \n

    \"\ preproc\": This performs basic DWI (and if necessary T1-weighted\ \ image) pre-processing\nbased on the raw BIDS data input, writing the resulting\ @@ -76166,11 +76168,11 @@ bids-apps/MRtrix3_connectome: \ performing\nappropriate inter-subject connection density normalisation as described\ \ in\nthis manuscript.\ \ Results are written to sub-directory\n\"MRtrix3_connectome-group\"\ - \ within the specified output directory.

    \n
  2. \n
\n

Execution platforms

\n

This\ - \ script can be utilised in one of three ways:

\n
    \n
  1. \n

    As a stand-alone\ - \ MRtrix3 script

    \n

    The script mrtrix3_connectome.py\ + \ within the specified output directory.

    \n
  2. \n
\n

Execution\ + \ platforms

\n

This script can be utilised in one of three ways:

\n
    \n\ +
  1. \n

    As a stand-alone MRtrix3 script

    \n

    The script mrtrix3_connectome.py\ \ can additionally be used outside of this Docker\ncontainer, as a stand-alone\ \ Python script build against the MRtrix3 Python libraries.\nUsing the\ \ script in this way requires setting the PYTHONPATH environment\ @@ -76203,50 +76205,52 @@ bids-apps/MRtrix3_connectome: \n

    The resulting container file \"MRtrix3_connectome.sif\"\ \ can be run as a stand-alone\nexecutable, as long as the system on which the\ \ file is executed has a version of\nSingularity installed that is compatible\ - \ with that of the system used to build the\ncontainer.

    \n
  2. \n
\nDocumentation\n\ -

The help page of the tool itself can be generated by executing the script without\n\ - providing any command-line options. The help page is additionally presented at\ - \ the\nbottom of this README page for reference. Documentation regarding the underlying\n\ - MRtrix3 tools can be found in the official\nMRtrix3 documentation. Additional information\n\ - may be found in the online\ - \ MRtrix3 community forum.

\n

Error Reporting

\n

Experiencing problems?\ - \ You can either post a private message to me on the\nMRtrix3 community forum, or you can report it\n\ - directly to the \n\n\n

Documentation

\n

The help page of the tool itself can be generated\ + \ by executing the script without\nproviding any command-line options. The help\ + \ page is additionally presented at the\nbottom of this README page for reference.\ + \ Documentation regarding the underlying\nMRtrix3 tools can be found\ + \ in the official\nMRtrix3 documentation. Additional information\nmay be found in the\ + \ online MRtrix3\ + \ community forum.

\n

Error Reporting

\n\ +

Experiencing problems? You can either post a private message to me on the\n\ + MRtrix3\ + \ community forum, or you can report it\ndirectly to the GitHub issues list.\nIn both cases, please include as much information as\ \ possible; this may include re-running\nthe script using the --debug\ \ option, which will provide additional information at the\nterminal, and preserve\ \ temporary files generated by the script within your target output\ndirectory,\ - \ which can be forwarded to the developer.

\n

Acknowledgements

\n

Development of this\ - \ tool was made possible through funding from the National Health\nand Medical\ - \ Research Council (NHMRC) of Australia.

\n

The developer acknowledges the\ - \ facilities and scientific and technical assistance of\nthe National Imaging\ - \ Facility, a National Collaborative Research Infrastructure Strategy\n(NCRIS)\ - \ capability, at the Florey Institute of Neuroscience and Mental Health.

\n\ -

The Florey Institute of Neuroscience and Mental Health acknowledges support\ - \ from the\nVictorian Government and in particular the funding from the Operational\ - \ Infrastructure\nSupport Grant.

\n

Robert Smith is supported by fellowship\ - \ funding from the National Imaging Facility (NIF),\nan Australian Government\ - \ National Collaborative Research Infrastructure Strategy (NCRIS)\ncapability.

\n\ -

Citation

\n\ -

When using this pipeline, please use the following snippet to acknowledge the\ - \ relevant\nwork (amend as appropriate depending on options used):

\n

Structural\ - \ connectomes were generated using the MRtrix3_connectome BIDS App (Smith\n\ - et al., 2019), which operates principally using tools provided in the MRtrix3\n\ - software package (Tournier et al., 2019; http://mrtrix.org). This included: DWI\ndenoising (Veraart et al.,\ - \ 2016), Gibbs ringing removal (Kellner et al., 2016),\npre-processing (Andersson\ - \ et al., 2003; Andersson and Sotiropoulos, 2016; Andersson\net al., 2016; (IF\ - \ USING EDDY_CUDA: Andersson et al., 2017)); and bias field correction\n(Tustison\ - \ et al., 2010 OR Zhang et al., 2001); inter-modal registration (Bhushan et al.,\n\ + \ which can be forwarded to the developer.

\n

Acknowledgements

\n\ +

Development of this tool was made possible through funding from the National\ + \ Health\nand Medical Research Council (NHMRC) of Australia.

\n

The developer\ + \ acknowledges the facilities and scientific and technical assistance of\nthe\ + \ National Imaging Facility, a National Collaborative Research Infrastructure\ + \ Strategy\n(NCRIS) capability, at the Florey Institute of Neuroscience and Mental\ + \ Health.

\n

The Florey Institute of Neuroscience and Mental Health acknowledges\ + \ support from the\nVictorian Government and in particular the funding from the\ + \ Operational Infrastructure\nSupport Grant.

\n

Robert Smith is supported\ + \ by fellowship funding from the National Imaging Facility (NIF),\nan Australian\ + \ Government National Collaborative Research Infrastructure Strategy (NCRIS)\n\ + capability.

\n

Citation

\n

When using this pipeline,\ + \ please use the following snippet to acknowledge the relevant\nwork (amend as\ + \ appropriate depending on options used):

\n

Structural connectomes were\ + \ generated using the MRtrix3_connectome BIDS App (Smith\net al., 2019),\ + \ which operates principally using tools provided in the MRtrix3\nsoftware\ + \ package (Tournier et al., 2019; http://mrtrix.org). This included: DWI\ndenoising (Veraart et al., 2016),\ + \ Gibbs ringing removal (Kellner et al., 2016),\npre-processing (Andersson et\ + \ al., 2003; Andersson and Sotiropoulos, 2016; Andersson\net al., 2016; (IF USING\ + \ EDDY_CUDA: Andersson et al., 2017)); and bias field correction\n(Tustison et\ + \ al., 2010 OR Zhang et al., 2001); inter-modal registration (Bhushan et al.,\n\ 2015); brain extraction (Smith, 2002 OR Iglesias et al., 2011), T1 tissue segmentation\n\ (Zhang et al., 2001; Smith, 2002; Patenaude et al., 2011; Smith et al., 2012);\ \ spherical\ndeconvolution (Tournier et al., 2004; Jeurissen et al., 2014); probabilistic\ @@ -76356,73 +76360,75 @@ bids-apps/MRtrix3_connectome: \ 2010, 50, 970-983\nZhang, Y.; Brady, M. & Smith, S. Segmentation of brain\ \ MR images through a hidden Markov random field model and the expectation-maximization\ \ algorithm. IEEE Transactions on Medical Imaging, 2001, 20, 45-57\n\n\ -

Help page

\n\ -

The following help page can equivalently be generated by executing the tool\ - \ without\nproviding any command-line arguments (within a container environment;\ - \ note interface\nis slightly different if run natively).

\n
\n

Synopsis

\n

Generate\ - \ structural connectomes based on diffusion-weighted and T1-weighted image data\ - \ using state-of-the-art reconstruction tools, particularly those provided in\ - \ MRtrix3

\n

Usage

\n
mrtrix3_connectome.py bids_dir output_dir analysis_level\
+    

Help page

\n

The following help page can equivalently be generated\ + \ by executing the tool without\nproviding any command-line arguments (within\ + \ a container environment; note interface\nis slightly different if run natively).

\n\ +
\n

Synopsis

\n

Generate structural connectomes\ + \ based on diffusion-weighted and T1-weighted image data using state-of-the-art\ + \ reconstruction tools, particularly those provided in MRtrix3

\n\ +

Usage

\n
mrtrix3_connectome.py bids_dir output_dir analysis_level\
     \ [ options ]\n
\n
    \n
  • \n

    bids_dir: The directory\ \ with the input dataset formatted according to the BIDS standard.

    \n
  • \n\
  • \n

    output_dir: The directory where the output files should be stored.

    \n\
  • \n
  • \n

    analysis_level: Level of analysis that will be performed;\ - \ options are: preproc, participant, group.

    \n
  • \n
\n

Description

\n

While preproc-level\ - \ analysis only requires data within the BIDS directory, participant-level analysis\ - \ requires that the output directory be pre-populated with the results from preproc-level\ - \ processing; similarly, group-level analysis requires that the output directory\ - \ be pre-populated with the results from participant-level analysis.

\n

The\ - \ operations performed by each of the three levels of analysis are as follows:

\n\ -

\"preproc\": DWI: Denoising; Gibbs ringing removal; motion, eddy current and\ - \ EPI distortion correction and outlier detection & replacement; brain masking,\ - \ bias field correction and intensity normalisation; rigid-body registration &\ - \ transformation to T1-weighted image. T1-weighted image: bias field correction;\ - \ brain masking.

\n

\"participant\": DWI: Response function estimation; FOD\ - \ estimation. T1-weighted image (if -parcellation is not none): Tissue segmentation;\ - \ grey matter parcellation. Combined (if -parcellation is not none, or -streamlines\ - \ is provided): Whole-brain streamlines tractography; SIFT2; connectome construction.

\n\ -

\"group\": Generation of FA-based population template; warping of template-based\ - \ white matter mask to subject spaces; calculation of group mean white matter\ - \ response function; scaling of connectomes based on white matter b=0 intensity,\ - \ response function used during participant-level analysis, and SIFT model proportioinality\ - \ coefficient; generation of group mean connectome.

\n

The label(s) provided\ - \ to the -participant_label and -session_label options correspond(s) to sub-<participant_label>\ - \ and ses-<session_label> from the BIDS spec (so they do not include\ - \ \"sub-\" or \"ses-\"). Multiple participants / sessions can be specified with\ - \ a space-separated list.

\n

For both preproc-level and participant-level\ - \ analyses, if no specific participants or sessions are nominated by the user\ - \ (or the user explicitly specifies multiple participants / sessions), the script\ - \ will process each of these in series. It is additionally possible for the user\ - \ to invoke multiple instances of this script in order to process multiple subjects\ - \ at once in parallel, ensuring that no single participant / session is being\ - \ processed in parallel, and that preproc-level output data are written fully\ - \ before commencing participant-level analysis.

\n

The -output_verbosity\ - \ option principally affects the participant-level analysis, modulating how many\ - \ derivative files are written to the output directory. Permitted values are from\ - \ 1 to 4: 1 writes only those files requisite for group-level analysis; 2 additionally\ - \ writes files typically useful for post-hoc analysis (the default); 3 additionally\ - \ generates files for enhanced connectome visualisation and copies the entire\ - \ whole-brain tractogram; 4 additionally generates a full copy of the script scratch\ - \ directory (with all intermediate files retained) to the output directory (and\ - \ this applies to all analysis levels)

\n

If running participant-level analysis\ - \ using the script as a standalone tool rather than inside the provided container,\ - \ data pertaining to atlas parcellations can no longer be guaranteed to be stored\ - \ at a specific location on the filesystem. In this case, the user will most likely\ - \ need to manually specify the location where the corresponding parcellation is\ - \ stored using the -atlas_path option.

\n

Options

\n
    \n
  • \n--output_verbosity
    The\ - \ verbosity of script output (number from 1 to 4).
  • \n
\n

Options that are relevant to participant-level analysis

\n
    \n
  • \n

    --parcellation
    The\ + \ options are: preproc, participant, group.

    \n
  • \n
\n

Description

\n\ +

While preproc-level analysis only requires data within the BIDS directory,\ + \ participant-level analysis requires that the output directory be pre-populated\ + \ with the results from preproc-level processing; similarly, group-level analysis\ + \ requires that the output directory be pre-populated with the results from participant-level\ + \ analysis.

\n

The operations performed by each of the three levels of analysis\ + \ are as follows:

\n

\"preproc\": DWI: Denoising; Gibbs ringing removal;\ + \ motion, eddy current and EPI distortion correction and outlier detection &\ + \ replacement; brain masking, bias field correction and intensity normalisation;\ + \ rigid-body registration & transformation to T1-weighted image. T1-weighted\ + \ image: bias field correction; brain masking.

\n

\"participant\": DWI: Response\ + \ function estimation; FOD estimation. T1-weighted image (if -parcellation is\ + \ not none): Tissue segmentation; grey matter parcellation. Combined (if -parcellation\ + \ is not none, or -streamlines is provided): Whole-brain streamlines tractography;\ + \ SIFT2; connectome construction.

\n

\"group\": Generation of FA-based population\ + \ template; warping of template-based white matter mask to subject spaces; calculation\ + \ of group mean white matter response function; scaling of connectomes based on\ + \ white matter b=0 intensity, response function used during participant-level\ + \ analysis, and SIFT model proportioinality coefficient; generation of group mean\ + \ connectome.

\n

The label(s) provided to the -participant_label and -session_label\ + \ options correspond(s) to sub-<participant_label> and ses-<session_label>\ + \ from the BIDS spec (so they do not include \"sub-\" or \"ses-\"). Multiple\ + \ participants / sessions can be specified with a space-separated list.

\n\ +

For both preproc-level and participant-level analyses, if no specific participants\ + \ or sessions are nominated by the user (or the user explicitly specifies multiple\ + \ participants / sessions), the script will process each of these in series. It\ + \ is additionally possible for the user to invoke multiple instances of this script\ + \ in order to process multiple subjects at once in parallel, ensuring that no\ + \ single participant / session is being processed in parallel, and that preproc-level\ + \ output data are written fully before commencing participant-level analysis.

\n\ +

The -output_verbosity option principally affects the participant-level analysis,\ + \ modulating how many derivative files are written to the output directory. Permitted\ + \ values are from 1 to 4: 1 writes only those files requisite for group-level\ + \ analysis; 2 additionally writes files typically useful for post-hoc analysis\ + \ (the default); 3 additionally generates files for enhanced connectome visualisation\ + \ and copies the entire whole-brain tractogram; 4 additionally generates a full\ + \ copy of the script scratch directory (with all intermediate files retained)\ + \ to the output directory (and this applies to all analysis levels)

\n

If\ + \ running participant-level analysis using the script as a standalone tool rather\ + \ than inside the provided container, data pertaining to atlas parcellations can\ + \ no longer be guaranteed to be stored at a specific location on the filesystem.\ + \ In this case, the user will most likely need to manually specify the location\ + \ where the corresponding parcellation is stored using the -atlas_path option.

\n\ +

Options

\n
    \n
  • \n--output_verbosity
    The\ + \ verbosity of script output (number from 1 to 4).
  • \n
\n

Options\ + \ that are relevant to participant-level analysis

\n
    \n
  • \n

    --parcellation
    The\ \ choice of connectome parcellation scheme (compulsory for participant-level analysis);\ \ options are: aal, aal2, brainnetome246fs, brainnetome246mni, craddock200, craddock400,\ \ desikan, destrieux, hcpmmp1, none, perry512, yeo7fs, yeo7mni, yeo17fs, yeo17mni.

    \n\ @@ -76430,24 +76436,24 @@ bids-apps/MRtrix3_connectome: \ generate for each subject (will be determined heuristically if not explicitly\ \ set).

    \n
  • \n
  • \n

    --template_reg software
    The choice\ \ of registration software for mapping subject to template space; options are:\ - \ ants, fsl.

    \n
  • \n
\n

Options that are relevant to both preproc-level and participant-level analyses

\n
    \n\ -
  • \n--t1w_preproc path
    Provide a path by which pre-processed\ + \ ants, fsl.

    \n
  • \n
\n

Options\ + \ that are relevant to both preproc-level and participant-level analyses

\n\ +
    \n
  • \n--t1w_preproc path
    Provide a path by which pre-processed\ \ T1-weighted image data may be found for the processed participant(s) / session(s)
  • \n\ -
\n

Options specific to the batch processing of participant data

\n
    \n
  • \n

    --participant_label
    The\ +

\n

Options\ + \ specific to the batch processing of participant data

\n
    \n
  • \n

    --participant_label
    The\ \ label(s) of the participant(s) that should be analyzed.

    \n
  • \n
  • \n

    --session_label
    The\ \ session(s) within each participant that should be analyzed.

    \n
  • \n
\n\ -

Standard options

\n\ -
' - stargazers_count: 23 + stargazers_count: 24 subscribers_count: 7 topics: [] - updated_at: 1699266828.0 + updated_at: 1702082584.0 ericcombiolab/Benchmark-metagenome-assemblers: data_format: 2 description: null @@ -99962,10 +99968,11 @@ fanglab/6mASCOPE: - Singularity full_name: fanglab/6mASCOPE latest_release: null - readme: "

6mASCOPE

\n\ -

Description

\n\ + readme: "

6mASCOPE

\n

Description

\n\

6mASCOPE is a toolbox to assess 6mA events in eukaryotic species using a quantitative\ \ deconvolution approach. By using a novel short-insert library (200~400bp) design\ \ with the PacBio sequencing Sequel II System, 6mASCOPE makes an effective use\ @@ -99974,137 +99981,143 @@ fanglab/6mASCOPE: \ approach, 6mASCOPE deconvolves the DNA molecules from a gDNA sample into species\ \ and genomic regions of interests, and sources of contamination. Using a rationally\ \ designed machine learning model, 6mASCOPE enables sensitive and reliable 6mA\ - \ quantification for each of the deconvolved composition.

\n

Authors' notes

\n

We are actively developing\ - \ 6mASCOPE to facilitate usage and broaden features. All feedback is more than\ - \ welcome. You can reach us on twitter (@iamfanggang and @kong_yimeng) or directly through the GitHub issues system.

\n

Installation

\n

6mASCOPE is distributed as a fully\ - \ functional image bypassing the need to install any dependencies others than\ - \ the virtualization software. We recommend using Singularity, which can be installed\ - \ on Linux systems and is often the preferred solution by HPC administrators (Quick Start). 6mASCOPE was tested extensively with Singularity\ - \ v3.6.4.

\n
module load\
-    \ singularity/3.6.4    # Required\
-    \ only singularity/3.6.4 is a dynamic environment module. \nsingularity\
-    \ pull 6mASCOPE.sif library://fanglabcode/default/6mascope:latest    # Download the image from cloud.sylabs.io;\
-    \ Make sure you have the network connection\nsingularity build --sandbox\
-    \ 6mASCOPE 6mASCOPE.sif     #\
-    \ Create a writable container named 6mASCOPE\nsingularity run --no-home\
-    \ -w 6mASCOPE    # Start an interactive\
-    \ shell to use 6mASCOPE, type `exit` to leave\ninit_6mASCOPE    #Inside the container; Only required once when\
-    \ start using 6mASCOPE\nsource run_6mASCOPE\
+    \ quantification for each of the deconvolved composition.

\n

Authors'\ + \ notes

\n

We are actively developing 6mASCOPE to facilitate usage and broaden\ + \ features. All feedback is more than welcome. You can reach us on twitter (@iamfanggang and\ + \ @kong_yimeng)\ + \ or directly through the GitHub issues system.

\n

Installation

\n

6mASCOPE\ + \ is distributed as a fully functional image bypassing the need to install any\ + \ dependencies others than the virtualization software. We recommend using Singularity,\ + \ which can be installed on Linux systems and is often the preferred solution\ + \ by HPC administrators (Quick Start). 6mASCOPE was tested extensively\ + \ with Singularity v3.6.4.

\n
module load singularity/3.6.4    # Required only singularity/3.6.4 is a dynamic environment module. \n\
+    singularity pull 6mASCOPE.sif library://fanglabcode/default/6mascope:latest  \
+    \  # Download the image from\
+    \ cloud.sylabs.io; Make sure you have the network connection\nsingularity\
+    \ build --sandbox 6mASCOPE 6mASCOPE.sif     # Create a writable container named 6mASCOPE\nsingularity\
+    \ run --no-home -w 6mASCOPE    #\
+    \ Start an interactive shell to use 6mASCOPE, type `exit` to leave\ninit_6mASCOPE\
+    \    #Inside the container; Only\
+    \ required once when start using 6mASCOPE\nsource\
+    \ run_6mASCOPE    #Inside the\
+    \ container; Required every time when running 6mASCOPE
\n

The\ + \ image retrieved from Sylab Cloud with singularity pull (e.g. 6mASCOPE.sif) is already\ + \ built and can be reused at will. Containers built with those instructions are\ + \ writable meaning that results from 6mASCOPE analysis can be retrieved when the\ + \ container is not running. Outputs for the following commands can be found at\ + \ ./path/to/6mASCOPE/home/6mASCOPE/. To re-run the same container:

\n\ +
singularity run --no-home\
+    \ -w 6mASCOPE    # Re-run container\
+    \ 6mASCOPE, type `exit` to leave\nsource run_6mASCOPE\
     \    #Inside the container; Required\
-    \ every time when running 6mASCOPE
\n

The image retrieved\ - \ from Sylab Cloud\ - \ with singularity pull (e.g. 6mASCOPE.sif) is already built and\ - \ can be reused at will. Containers built with those instructions are writable\ - \ meaning that results from 6mASCOPE analysis can be retrieved when the container\ - \ is not running. Outputs for the following commands can be found at ./path/to/6mASCOPE/home/6mASCOPE/.\ - \ To re-run the same container:

\n
singularity run --no-home -w 6mASCOPE    # Re-run container 6mASCOPE, type `exit` to leave\nsource run_6mASCOPE    #Inside the container; Required every time when running 6mASCOPE
\n\ -

Tool showcase

\n\ + \ every time when running 6mASCOPE
\n

Tool showcase

\n\

To showcase the toolbox applications, we provide examples for the analysis\ \ of the Drosophila ~45min embryo dataset presented in our manuscript (Fig 5).\ \ The dataset can be downloaded with the following commands from within a 6mASCOPE\ - \ container: 6mASCOPE get_test_data

\n

Contamination estimation

\n

Goal

\n

To get an idea about\ - \ the overall contamination of a gDNA sample. This step helps users define the\ - \ composition of a gDNA sample using a metagenomic approach to assign reads to\ - \ different species.

\n

Description:

\n

For a given CCS dataset generated from short-insert\ + \ container: 6mASCOPE get_test_data

\n

Contamination\ + \ estimation

\n

Goal

\n

To get an idea about the overall contamination\ + \ of a gDNA sample. This step helps users define the composition of a gDNA sample\ + \ using a metagenomic approach to assign reads to different species.

\n

Description:

\n

For a given CCS dataset generated from short-insert\ \ library, 6mASCOPE will examine if there are contaminating species and calculate\ \ the proportion of reads mapped to the reference and top 50 contaminated species\ - \ from reads that do not map to the eukaryotic species of interest.

\n

Inputs:

\n
    \n\ -
  1. CCS reads file capturing all the genetic material in a gDNA sample (.fasta,\ - \ pre-computed in the following example)
  2. \n
  3. Eukaryotic reference of genome\ - \ of interest (.fasta)
  4. \n
\n

Outputs:

\n

For a given CCS dataset generated from short-insert\ - \ library, 6mASCOPE will examine if there are contaminating species\ - \ and calculate the proportion of reads mapped to the reference and top 50 contaminated\ - \ species from reads that do not map to the eukaryotic species of interest.

\n\ -
Example of the Output:
\n
Remove 8491 possible inter-species\
-    \ chimeric reads for further analysis\n#total_CCS\tmapped_to_goi\tcontaminants\n\
-    666159\t640345 (96.1249%)\t25814 (3.87505%)\n\nTop 50 mapped species outside goi\
-    \ reference\n#Count\tSpecies\n  10836 Saccharomyces cerevisiae\n   2413 Acetobacter\
-    \ tropicalis\n   1524 Acetobacter pasteurianus\n   1479 Lactobacillus plantarum\n\
-    \    882 Acetobacter sp.\n ...\n
\n

(Full species list can be viewed\ - \ in test.contam.estimate.txt)

\n
Example commands:
\n
6mASCOPE contam\
-    \ -c test.ccs.fasta -r test.ref.fasta -o test.contam.estimate.txt\n
\n\ + \ from reads that do not map to the eukaryotic species of interest.

\n

Inputs:

\n
    \n
  1. CCS reads file capturing all the genetic material\ + \ in a gDNA sample (.fasta, pre-computed in the following example)
  2. \n
  3. Eukaryotic\ + \ reference of genome of interest (.fasta)
  4. \n
\n

Outputs:

\n\ +

For a given CCS dataset generated from short-insert library, 6mASCOPE\ + \ will examine if there are contaminating species and calculate the proportion\ + \ of reads mapped to the reference and top 50 contaminated species from reads\ + \ that do not map to the eukaryotic species of interest.

\n
Example\ + \ of the Output:
\n
Remove 8491 possible inter-species chimeric\
+    \ reads for further analysis\n#total_CCS\tmapped_to_goi\tcontaminants\n666159\t\
+    640345 (96.1249%)\t25814 (3.87505%)\n\nTop 50 mapped species outside goi reference\n\
+    #Count\tSpecies\n  10836 Saccharomyces cerevisiae\n   2413 Acetobacter tropicalis\n\
+    \   1524 Acetobacter pasteurianus\n   1479 Lactobacillus plantarum\n    882 Acetobacter\
+    \ sp.\n ...\n
\n

(Full species list can be viewed in test.contam.estimate.txt)

\n\ +
Example commands:
\n
6mASCOPE\
+    \ contam -c test.ccs.fasta -r test.ref.fasta -o test.contam.estimate.txt\n
\n\

In this example, test.ccs.fasta includes CCS reads (674,650) from\ \ the Drosophila ~45min embryo reads dataset described in our manuscript and pre-filtered\ \ with command 6mASCOPE ccs. Using 5 cores, runtime is ~12m51s. The\ \ output shows ~3.9% CCS reads come from contaminated sources other than Drosophila\ \ melanogaster, the genome of interest (goi). Please be noted, blastn is embedded\ - \ within this step, which will need at least 32-64G RAM.

\n

6mA analysis using quantitative deconvolution

\n

Goal:

\n

For each source determined in 6mASCOPE\ - \ contam, this step will quantify the 6mA/A level and calculate the 6mA\ - \ contribution (%) of each source to the total 6mA abundance in the gDNA sample.

\n\ -

Inputs:

\n\ -
    \n
  1. The same CCS reads file as explained above for Contamination Estimation\ - \ (.fasta).
  2. \n
  3. IPD and QV information of the CCS reads (pre-computed in\ - \ the following example, ; this can be generated for new data with 6mASCOPE\ - \ ipd command, as explained in detailed tutorial).
  4. \n
  5. User defined\ - \ groups besides the genome of interest. Examples as shown below. (Left columns:\ - \ subgroup name. Right columns: contamination sources, use vertical line if multiple\ - \ sources included within one subgroup).
  6. \n
\n
Saccharomyces\
-    \   Saccharomyces\nAcetobacter     Acetobacter|Komagataeibacter\nLactobacillus\
-    \   Lactobacillus\n
\n

Outputs:

\n

A table including the following information:\ - \ the proportion (%) of reads from each source out of the total number of reads;\ - \ source-specific 6mA/A level with 95% confidence intervals (log10-transformed),\ + \ within this step, which will need at least 32-64G RAM.

\n

6mA analysis\ + \ using quantitative deconvolution

\n

Goal:

\n

For each source\ + \ determined in 6mASCOPE contam, this step will quantify the 6mA/A\ + \ level and calculate the 6mA contribution (%) of each source to the total 6mA\ + \ abundance in the gDNA sample.

\n

Inputs:

\n
    \n
  1. The same\ + \ CCS reads file as explained above for Contamination Estimation (.fasta).
  2. \n\ +
  3. IPD and QV information of the CCS reads (pre-computed in the following example,\ + \ ; this can be generated for new data with 6mASCOPE ipd command,\ + \ as explained in detailed tutorial).
  4. \n
  5. User defined groups besides the\ + \ genome of interest. Examples as shown below. (Left columns: subgroup name. Right\ + \ columns: contamination sources, use vertical line if multiple sources included\ + \ within one subgroup).
  6. \n
\n
Saccharomyces   Saccharomyces\n\
+    Acetobacter     Acetobacter|Komagataeibacter\nLactobacillus   Lactobacillus\n\
+    
\n

Outputs:

\n

A table including the following\ + \ information: the proportion (%) of reads from each source out of the total number\ + \ of reads; source-specific 6mA/A level with 95% confidence intervals (log10-transformed),\ \ and contribution (%) of each source to the total 6mA abundance in the gDNA sample\ - \ (as presented in the manuscript Figure 5A, B, C)

\n

Example commands:

\n
6mASCOPE\
-    \ quant -c test.ccs.fasta -i test.IPD.out.A -o test -r test.ref.fasta -s subgroup.txt\n\
-    
\n

In this example, the file test.IPD.out.A includes\ - \ the pre-calculated IPD and QV information on the CCS molecules (can be generated\ - \ with 6mASCOPE ipd). Only Adenines were included here to to reduce\ - \ computational time and ease evaluation. subgroup.txt includes the\ - \ pre-defined main contamination groups, inferred from the top mapped species\ - \ and blast output from 6mASCOPE contam. Using 5 cores, runtime is\ - \ ~13m17s.

\n

Example output:

\n
     #Subgroup   count  ReadsProportion\
-    \  6mAlevel(ppm)  6mAlevel(log10)  UpCI  DownCI  subtotal(ppm)  contribution(%)\n\
-    \           goi  640345           0.9612         2.0417            -5.69  -5.0\
-    \    -6.0         1.9625           1.4431\n Saccharomyces   11011           0.0165\
-    \        45.7088            -4.34  -3.9    -6.0         0.7542           0.5546\n\
-    \   Acetobacter    5757           0.0086      5495.4087            -2.26  -2.0\
-    \    -2.5        47.2605          34.7522\n Lactobacillus    1517           0.0023\
-    \       977.2372            -3.01  -2.7    -3.3         2.2476           1.6528\n\
-    \        others    7529           0.0113      7413.1024            -2.13  -1.9\
-    \    -2.4        83.7681          61.5974\n
\n

\n \n

Example\ + \ commands:

\n
6mASCOPE quant -c test.ccs.fasta -i test.IPD.out.A\
+    \ -o test -r test.ref.fasta -s subgroup.txt\n
\n

In this example,\ + \ the file test.IPD.out.A includes the pre-calculated IPD and QV\ + \ information on the CCS molecules (can be generated with 6mASCOPE ipd).\ + \ Only Adenines were included here to to reduce computational time and ease evaluation.\ + \ subgroup.txt includes the pre-defined main contamination groups,\ + \ inferred from the top mapped species and blast output from 6mASCOPE contam.\ + \ Using 5 cores, runtime is ~13m17s.

\n

Example\ + \ output:

\n
     #Subgroup   count  ReadsProportion  6mAlevel(ppm)\
+    \  6mAlevel(log10)  UpCI  DownCI  subtotal(ppm)  contribution(%)\n           goi\
+    \  640345           0.9612         2.0417            -5.69  -5.0    -6.0     \
+    \    1.9625           1.4431\n Saccharomyces   11011           0.0165        45.7088\
+    \            -4.34  -3.9    -6.0         0.7542           0.5546\n   Acetobacter\
+    \    5757           0.0086      5495.4087            -2.26  -2.0    -2.5     \
+    \   47.2605          34.7522\n Lactobacillus    1517           0.0023       977.2372\
+    \            -3.01  -2.7    -3.3         2.2476           1.6528\n        others\
+    \    7529           0.0113      7413.1024            -2.13  -1.9    -2.4     \
+    \   83.7681          61.5974\n
\n

\n \"The\n

\n1. The % of total CCS reads mapped to different subgroups. Left:\ @@ -100129,23 +100142,24 @@ fanglab/6mASCOPE: \ abundance in the gDNA sample. CCS reads mapped to the D. melanogaster genome\ \ only explains 1.4% of the total 6mA events in the gDNA sample (green).\n

These\ \ figures can be drawn with sh ~/code/draw_example.sh test.6mASCOPE.txt.

\n\ -

Documentation

\n\ -

For a comprehensive description of\_6mASCOPE including installation guide,\ - \ data preprocessing and a detailed tutorial, including how to apply 6mASCOPE\ - \ to your own datasets, please refer to the\_complete documentation .

\n

Citation

\n

Yimeng Kong, Lei Cao, Gintaras\ - \ Deikus, Yu Fan, Edward A. Mead, Weiyi Lai, Yizhou Zhang, Raymund Yong, Robert\ - \ Sebra, Hailin Wang, Xue-Song Zhang & Gang Fang. Critical assessment of DNA\ - \ adenine methylation in eukaryotes using quantitative deconvolution. Science\ - \ (2022). doi:10.1126/science.abe7489.

\n" - stargazers_count: 5 +

Documentation

\n

For a comprehensive description\ + \ of\_6mASCOPE including installation guide, data preprocessing and a detailed\ + \ tutorial, including how to apply 6mASCOPE to your own datasets, please refer\ + \ to the\_complete documentation .

\n

Citation

\n\ +

Yimeng Kong, Lei Cao, Gintaras Deikus, Yu Fan, Edward A. Mead, Weiyi Lai, Yizhou\ + \ Zhang, Raymund Yong, Robert Sebra, Hailin Wang, Xue-Song Zhang & Gang Fang.\ + \ Critical assessment of DNA adenine methylation in eukaryotes using quantitative\ + \ deconvolution. Science (2022). doi:10.1126/science.abe7489.

\n" + stargazers_count: 6 subscribers_count: 6 topics: [] - updated_at: 1696631454.0 + updated_at: 1700119528.0 fanglab/nanodisco: data_format: 2 description: 'nanodisco: a toolbox for discovering and exploiting multiple types @@ -101344,10 +101358,10 @@ fempar/fempar: description: Finite Element Multiphysics PARallel solvers. Official mirror from https://gitlab.com/fempar/fempar filenames: - - Containers/Singularity/Singularity.gnu-release_p4est-parallel - Containers/Singularity/Singularity.gnu-debug_p4est-parallel - - Containers/Singularity/Singularity.gnu-release_p4est-serial + - Containers/Singularity/Singularity.gnu-release_p4est-parallel - Containers/Singularity/Singularity.gnu-debug_p4est-serial + - Containers/Singularity/Singularity.gnu-release_p4est-serial full_name: fempar/fempar latest_release: null readme: "

' - stargazers_count: 98 + stargazers_count: 100 subscribers_count: 23 topics: [] - updated_at: 1699309650.0 + updated_at: 1702766950.0 fksato/caffe2_singularity: data_format: 2 description: null @@ -107643,7 +107657,7 @@ github-linguist/linguist: filenames: - samples/Singularity/filenames/Singularity full_name: github-linguist/linguist - latest_release: v7.27.0 + latest_release: v7.28.0 readme: "

Linguist

\n

vendor/README.md\ \ lists the repository for each grammar.

\n

All other files are covered by\ \ the MIT license, see LICENSE.

\n" - stargazers_count: 11433 + stargazers_count: 11444 subscribers_count: 505 topics: - syntax-highlighting - language-grammars - language-statistics - linguistic - updated_at: 1702266766.0 + updated_at: 1702868224.0 github/linguist: data_format: 2 description: Language Savant. If your repository's language is being reported incorrectly, @@ -110718,11 +110732,11 @@ h3abionet/h3avarcall: data_format: 2 description: H3A variant calling pipeline filenames: + - containers/Singularity.trimmomatic - containers/Singularity.bwa + - containers/Singularity.multiqc - containers/Singularity.fastqc - - containers/Singularity.trimmomatic - containers/Singularity.gatk - - containers/Singularity.multiqc full_name: h3abionet/h3avarcall latest_release: null readme: "

\"Build

\n

cylon

\n

Virus assembly module used by viridian

\n\ -

Important

\n\ + \ alt=\"Build Status\" style=\"max-width: 100%;\">

\n

cylon

\n\ +

Virus assembly module used by viridian

\n

Important

\n\

We recommend that you use\nViridian workflow instead of\nthis repository. This repository is intended\ \ to be run by\nViridian workflow, not to be used as a stand-alone tool.\nViridian\ \ workflow provides a complete end-to-end pipeline for\ngenerating a consensus\ - \ sequence from reads.

\n

Install

\n

From source

\n

These must be installed and in your $PATH:

\n\ -
\n

Examples

\n

See examples.

\n

Installation

\n

beautier\ \ can be installed:

\n\n

CRAN

\n

For the latest CRAN version:

\n\ -
install.packages(\"beautier\")
\n\ -

GitHub, master branch

\n

For the latest stable\ - \ version:

\n
remotes::install_github(\"ropensci/beautier\")
\n

GitHub, develop\ - \ branch

\n\ + \ GitHub, develop branch\n\n

CRAN

\n

For the latest\ + \ CRAN version:

\n
install.packages(\"beautier\")
\n

GitHub,\ + \ master branch

\n

For the latest stable version:

\n
remotes::install_github(\"ropensci/beautier\")
\n\ +

GitHub, develop branch

\n\

For the bleeding-edge version:

\n
remotes::install_github(\"ropensci/beautier\", ref = \"develop\")
\n

FAQ

\n

See FAQ.

\n

Supported

\n

This works, and the interface is unlikely to change.

\n\ -
    \n
  • 1 DNA alignment
  • \n
  • Site models:\n
      \n
    • JC69
    • \n
    • HKY
    • \n\ -
    • TN93
    • \n
    • GTR
    • \n
    \n
  • \n
  • Clock models:\n
      \n
    • Strickt
    • \n\ -
    • Relaxed log-normal
    • \n
    \n
  • \n
  • Tree models:\n
      \n
    • Yule
    • \n\ -
    • Birth-Death
    • \n
    • Coalescent Bayesian Skyline
    • \n
    • Coalescent Constant\ - \ Population
    • \n
    • Coalescent Exponential Population
    • \n
    \n
  • \n\ -
  • Handle missing data: simply use a dash (\xB4-\xB4) as a sequence\nin a FASTA\ - \ file
  • \n
\n

Experimental

\n

This works partially, and the interface\ - \ may change as well.

\n

Tip dating

\n

The tip dates file is a file\nthat needs to not have column,\ - \ nor row names.\nThe columns need to be tab separated.

\n

See \")\n

FAQ

\n\ +

See FAQ.

\n

Supported

\n\ +

This works, and the interface is unlikely to change.

\n
    \n
  • 1 DNA alignment
  • \n\ +
  • Site models:\n
      \n
    • JC69
    • \n
    • HKY
    • \n
    • TN93
    • \n
    • GTR
    • \n\ +
    \n
  • \n
  • Clock models:\n
      \n
    • Strickt
    • \n
    • Relaxed log-normal
    • \n\ +
    \n
  • \n
  • Tree models:\n
      \n
    • Yule
    • \n
    • Birth-Death
    • \n
    • Coalescent\ + \ Bayesian Skyline
    • \n
    • Coalescent Constant Population
    • \n
    • Coalescent\ + \ Exponential Population
    • \n
    \n
  • \n
  • Handle missing data: simply use\ + \ a dash (\xB4-\xB4) as a sequence\nin a FASTA file
  • \n
\n

Experimental

\n

This works partially, and the interface may\ + \ change as well.

\n

Tip dating

\n

The tip dates\ + \ file is a file\nthat needs to not have column, nor row names.\nThe columns need\ + \ to be tab separated.

\n

See here\nfor an example, of which the first rows are shown here:

\n
KF767106_Indonesia_1976_VII\t\
     1976\nKF767104_Indonesia_1988_VII\t1988\nKF767105_Indonesia_1988_VII\t1988\nAY288998_Indonesia_1990_VII\t\
     1990\n
\n

In the future, there probably will be a \xB4to_tipdates_file\xB4\ - \ function,\nto create a temporary tipdates file from a table.

\n

Missing features/unsupported

\n

beautier cannot do everything BEAUti\ - \ can.

\n

Here are some missing or (yet) unsupported features,\nsome are\ - \ linked to an Issue:

\n\n

There is a feature I miss

\n\ +

See CONTRIBUTING, at Submitting use cases

\n\ +

I want to collaborate

\n

See\ + \ CONTRIBUTING, at 'Submitting code'

\n

I think I have found a bug

\n\

See CONTRIBUTING, at 'Submitting bugs'

\n\ -

There's something else I want\ - \ to say

\n\ -

Sure, just add an Issue. Or send an email.

\n

External links

\n\n

References

\n

Article about babette:

\n\ -
    \n
  • Bilderbeek, Rich\xE8l JC, and Rampal S. Etienne. \"babette:\ - \ BEAUti 2, BEAST 2 and Tracer for R.\" Methods in Ecology and Evolution (2018).\ - \ https://doi.org/10.1111/2041-210X.13032\n\ -
  • \n
\n

FASTA files anthus_aco.fas and anthus_nd2.fas\ - \ from:

\n
    \n
  • Van Els, Paul, and Heraldo V. Norambuena. \"A revision\ - \ of species limits in Neotropical pipits Anthus based on multilocus genetic and\ - \ vocal data.\" Ibis.
  • \n
\n

FASTA file G_VII_pre2003_msa.fas\ - \ from:

\n
    \n
  • Durr, PA; Wibowo, MH; Tabbu, CR; Asmara, W; Selleck, P;\ - \ Wang, J; Broz, I; Graham, K.; Dimitrov, K and Afonso, C. (in preparation). Phylodynamics\ - \ of Genotype VII Newcastle disease virus in Indonesia.
  • \n
\n

There's\ + \ something else I want to say\n

Sure, just add an Issue. Or send an email.

\n\ +

External links

\n\n

References

\n

Article about\ + \ babette:

\n\n\ +

FASTA files anthus_aco.fas and anthus_nd2.fas from:

\n\ +
    \n
  • Van Els, Paul, and Heraldo V. Norambuena. \"A revision of species limits\ + \ in Neotropical pipits Anthus based on multilocus genetic and vocal data.\" Ibis.
  • \n\ +
\n

FASTA file G_VII_pre2003_msa.fas from:

\n
    \n
  • Durr,\ + \ PA; Wibowo, MH; Tabbu, CR; Asmara, W; Selleck, P; Wang, J; Broz, I; Graham,\ + \ K.; Dimitrov, K and Afonso, C. (in preparation). Phylodynamics of Genotype VII\ + \ Newcastle disease virus in Indonesia.
  • \n
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\"ropensci_footer\"

\n" - stargazers_count: 12 + stargazers_count: 13 subscribers_count: 3 topics: - r - r-package - rstats - updated_at: 1689862358.0 + updated_at: 1698919812.0 ropensci/mauricer: data_format: 2 description: Install BEAST2 packages from R @@ -172397,19 +172408,19 @@ rses-singularity/all-images: data_format: 2 description: null filenames: - - caffe2/Singularity - - tensorflow-gpu/Singularity - - tensorflow-cpu/Singularity - - theano/Singularity + - caffe-gpu/Singularity - neurokernel/Singularity - neurokernel/SingularityPC - - torch/Singularity + - cuda/Singularity - freesurfer/Singularity + - tensorflowgpu-theano-keras-pytorch/Singularity + - theano/Singularity + - tensorflow-cpu/Singularity + - torch/Singularity - digits/Singularity - - caffe-gpu/Singularity - - cuda/Singularity + - tensorflow-gpu/Singularity - caffe-cpu/Singularity - - tensorflowgpu-theano-keras-pytorch/Singularity + - caffe2/Singularity full_name: rses-singularity/all-images latest_release: null readme: '

.

' - stargazers_count: 582 + stargazers_count: 587 subscribers_count: 14 topics: - containers - hpc - linux - updated_at: 1702018447.0 + updated_at: 1702862877.0 sylvainschmitt/singularity-octopus: data_format: 2 description: 'octopus Singularity container ' @@ -195002,10 +195013,10 @@ vistle/singularity: data_format: 2 description: Singularity containers for running COVISE, OpenCOVER, and Vistle filenames: - - Singularity.covise - Singularity.covise-deps - - Singularity.vistle-client - Singularity.vistle-server + - Singularity.vistle-client + - Singularity.covise - Singularity.centos7 full_name: vistle/singularity latest_release: null @@ -197912,8 +197923,8 @@ wheaton5/souporcell: data_format: 2 description: Clustering scRNAseq by genotypes filenames: - - Singularity - Singularity.2.5 + - Singularity - Singularity.2.0 full_name: wheaton5/souporcell latest_release: v2.5 @@ -198196,7 +198207,7 @@ wheaton5/souporcell: \ which alt and ref matrix were created\n
\n

So generally

\n\
consensus.py -c clusters.tsv -a alt.mtx -r ref.mtx --soup_out soup.txt\
     \ -v <freebayes vcf> --vcf_out cluster_genotypes.vcf --output_dir .\n
\n" - stargazers_count: 137 + stargazers_count: 140 subscribers_count: 10 topics: - scrna-seq @@ -198205,7 +198216,7 @@ wheaton5/souporcell: - bioinformatics - computational-biology - genomics - updated_at: 1700859338.0 + updated_at: 1702347004.0 willgpaik/centos7_aci: data_format: 2 description: Centos 7 base image for ACI @@ -199648,10 +199659,10 @@ yaaig-ufrgs/NeuralFastDownward-FSM: description: "\U0001F35D Code for the paper \"Understanding Sample Generation Strategies\ \ for Learning Heuristic Functions in Classical Planning.\"" filenames: - - misc/releases/latest/Singularity - misc/releases/19.12/Singularity.19.12 - misc/releases/19.06/Singularity.19.06 - misc/releases/20.06/Singularity.20.06 + - misc/releases/latest/Singularity full_name: yaaig-ufrgs/NeuralFastDownward-FSM latest_release: null readme: "