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After installing the latest version of ALE via Bioconda, I have followed these steps.
Step 1: Mapping PacBio reads with Minialing (raw reads input was fasta file)
Step 2: Converting SAM to BAM with Samtools 1.9
Step 3: Executing ALE with this command "ALE aln_ArbQM_MiAln.sorted.bam ArbQM_WDGRC_RN.fasta ALE_ArbQM.ale"
After this, I was expecting to see ALE_ArbQM.ale but there were only two files (please see two txt files).
Dear ALE,
After installing the latest version of ALE via Bioconda, I have followed these steps.
Step 1: Mapping PacBio reads with Minialing (raw reads input was fasta file)
Step 2: Converting SAM to BAM with Samtools 1.9
Step 3: Executing ALE with this command "ALE aln_ArbQM_MiAln.sorted.bam ArbQM_WDGRC_RN.fasta ALE_ArbQM.ale"
After this, I was expecting to see ALE_ArbQM.ale but there were only two files (please see two txt files).
ALE_AbQM.txt
ALE_ArbQM.ale.param.txt
Just wondering, did I miss something? If not, how can I interpret the outcome from the two txt files?
Many thanks in advance!
Cheers,
Taek
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