diff --git a/PatchWarp/patchwarp_affine.m b/PatchWarp/patchwarp_affine.m
index 48f35d8..43cbc17 100644
--- a/PatchWarp/patchwarp_affine.m
+++ b/PatchWarp/patchwarp_affine.m
@@ -52,10 +52,10 @@ function patchwarp_affine(source_path, save_path, n_ch, align_ch, affine_norm_ra
 % transform = 'affine';
 
 disp('Loading downsampled_perstack file...')
-[stack_downsampled_perstack, info_downsampled_perstack] = read_tiff(fn_downsampled_perstack, align_ch, 1, network_temp_copy);
+[stack_downsampled_perstack, info_downsampled_perstack] = read_tiff(fn_downsampled_perstack, 1, 1, network_temp_copy);
 if ismatrix(stack_downsampled_perstack)
     downsampled_nframes_used = 1;     % downsampled_[frame_num].tif is used instead of downsampled_perstack.tif if there is only 1 tiff stack file.
-    [stack_downsampled_perstack, ~] = read_tiff(fullfile(source_path,'downsampled',fn_downsampled_name), align_ch, 1, network_temp_copy);
+    [stack_downsampled_perstack, ~] = read_tiff(fullfile(source_path,'downsampled',fn_downsampled_name), 1, 1, network_temp_copy);
 else
     downsampled_nframes_used = 0;
 end