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I have got ~70GB of Illumina data from Wolbachia-positive mosquito sample, with a view to assemble the Wolbachia genome. After QC, I mapped the reads to wPip genome (nearest Wolbachia spp) and extracted the mapped reads for assembly using Unicycler. Unicycler showed "successful run", but once I checked the files, the assembly.fasta file showed empty! I have tried both normal and Bold modes, but the results are same! Can you please advise, how to fix it?
I have got ~70GB of Illumina data from Wolbachia-positive mosquito sample, with a view to assemble the Wolbachia genome. After QC, I mapped the reads to wPip genome (nearest Wolbachia spp) and extracted the mapped reads for assembly using Unicycler. Unicycler showed "successful run", but once I checked the files, the assembly.fasta file showed empty! I have tried both normal and Bold modes, but the results are same! Can you please advise, how to fix it?
unicycler.log
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