Qubit_2mL_Tubes

from opentrons import labware, instruments, robot


# labware & instrument setup
tiprack_200ul = labware.load('tiprack-200ul', '10')
tiprack_10ul = labware.load('tiprack-10ul', '11')

# Qubit mix is placed in a 50mL conical in A3
tuberack_buffer = labware.load('tube-rack-15_50ml', '7')

# For now, qubit tubes are placed into deepwelll 96 well plates, in odd columns only
plate_tubes_A = labware.load('96-PCR-tall', '4')
plate_tubes_B = labware.load('96-PCR-tall', '5')

# These are precious. So keep them where they won't get dripped on
tuberack_DNA_1 = labware.load('tube-rack-2ml', '1')
tuberack_DNA_2 = labware.load('tube-rack-2ml', '2')
tuberack_DNA_3 = labware.load('tube-rack-2ml', '3')


s300 = instruments.P300_Single(
                               tip_racks=[tiprack_200ul],
                               mount="right"
                               )

s10 = instruments.P10_Single(
                             tip_racks=[tiprack_10ul],
                             mount="left"
                             )


# distribute 198ul qubit buffer to 96 tubes
# Do this first, otherwise the 2uL does not actually get into the tube
# It gets a little drippy, but I dont care so much at this stage.
s300.distribute(198, tuberack_buffer['A3'], plate_tubes_A.cols(0,2,4,6,8,10))
s300.distribute(198, tuberack_buffer['A3'], plate_tubes_B.cols(0,2,4))

# add 2ul library to each tube
# Do this second, I tried to do it first with a blow out, but on the p10, the blow out command seems to cause the pipette to fall right off!
s10.transfer(2, tuberack_DNA_1(), plate_tubes_A.cols(0,2,4), mix_before=(3, 5), new_tip='always')
s10.transfer(2, tuberack_DNA_2(), plate_tubes_A.cols(6,8,10), mix_before=(3, 5), new_tip='always')
s10.transfer(2, tuberack_DNA_3(), plate_tubes_B.cols(0,2,4), mix_before=(3, 5), new_tip='always')