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ppower step failed because of Remote I/O error #6

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red-t opened this issue Oct 19, 2020 · 0 comments
Open

ppower step failed because of Remote I/O error #6

red-t opened this issue Oct 19, 2020 · 0 comments

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@red-t
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red-t commented Oct 19, 2020

Hi, it seems that I've managed to run LiRA successfully with slurm for cancer's data (from Li et al.). Here is the summary information:

information:
Polymorphic germline SNVs: c(onek = 39807)
Properly phased germline SNVs: c(germline.phased = 1027)
Improperly phased germline SNVs: c(germline.misphased = 4)
Properly phased sSNV candidates: c(somatic.phased = 96)
Improperly phased sSNV candidates: c(somatic.misphased = 43)
sSNV rate (Nuc/Gbp): 30726
 Lower bound (98%): 16973
 Upper bound (98%): 45440
Composite coverage threshold: 2
Total passing sSNVs: 31
Estimated total # of sSNVs: 198308
Estimated sensitivity: 0.0001563224882506
Estimated FPR: 0.00

But, when running lira ppower for single neuron's sequence data (BAM file with 35G~237G size), some I/O errors occur. Here is part of the errors:

[E::hts_open] fail to open file '.bulk.6248_200804_cer/power_bams/100.bulk.6248_200804_cer.bam'
samtools: Could not open ".bulk.6248_200804_cer/power_bams/100.bulk.6248_200804_cer.bam": Remote I/O error

In local.power.function, it will apply samtools view with mapping result as input. When using batch.size=10, It seems that, function submit.jobs will submit 10 bash scripts for each slurm job? And each bash script will call local.power.function. Is it possible that this is the cause of the error? And should I use a smaller batch.size?

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